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EC number: 224-924-0 | CAS number: 4553-89-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study
- Justification for type of information:
- Experimental test result performed using standard OECD test guidelines
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Principles of method if other than guideline:
- This study was designed to assess the effect of the test item disodium 4,4'-[[2,4-dihydroxy-5-(hydroxymethyl)-1,3-phenylene]bis(azo)]bisnaphthalene-1-sulphonate (CAS No. 4553-89-3) on Danio rerio. The study was conducted in accordance with OECD Guideline 203 (Fish, Acute Toxicity Test).
- GLP compliance:
- no
- Specific details on test material used for the study:
- - Name of test material: disodium 4,4'-[[2,4-dihydroxy-5-(hydroxymethyl)-1,3-phenylene]bis(azo)]bisnaphthalene-1-sulphonate
- Molecular formula: C27H18N4Na2O9S2
- Molecular weight: 652.57 g/mol
- Smiles notation: c12c(c(\N=N\c3c(c(\N=N\c4c5c(c(S(=O)(=O)[O-])cc4)cccc5)cc(c3O)CO)O)ccc1S(=O)(=O)[O-])cccc2.[Na+].[Na+]
- InChI: 1S/C27H20N4O9S2.2Na/c32-14-15-13-22(30-28-20-9-11-23(41(35,36)37)18-7-3-1-5-16(18)20)27(34)25(26(15)33)31-29-21-10-12-24(42(38,39)40)19-8-4-2-6-17(19)21;;/h1-13,32-34H,14H2,(H,35,36,37)(H,38,39,40);;/q;2*+1/p-2/b30-28+,31-29+;;
- Substance type: Organic
- Physical state: Solid
- Batch No.: FG/14-15/1097
- Physical Appearance: Dark brown powder
- Purity: 79.4 % (dye content by GC)
- Stability: Stable in recommended storage conditions
- Storage Conditions: 1. Ambient Temp (23 to 27 °C), 2. In Refrigerator (4 °C), 3. In Freezer (-10 To 0 °C)
- Solubility in water: 99418 mg/L - Analytical monitoring:
- not specified
- Vehicle:
- no
- Details on test solutions:
- The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 1 gm of the test substance in 1 liter of potable water (passed through reverse osmosis system) with continuous stirring for achieving test concentrations of 6.25 mg/L, 12.5mg/L,25mg/L, 50mg/L, 100mg/L, respectively
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: Zebra Fish (Danio rerio)
- Source: Asim Fancy Fish Aquarium, Dharampeth, Nagpur, MS (India)
- Length at study initiation (length definition, mean, range and SD):average 2.46 cm
- Weight at study initiation (mean and range, SD):0.0942 g (approx.)
- Physical conditions: No abnormalities - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Hardness:
- 62.5mg of CaCO3
- Test temperature:
- 22°C
- pH:
- 7.3
- Dissolved oxygen:
- 7.9 mg/L
- Nominal and measured concentrations:
- 6.25 mg/L, 12.5mg/L,25mg/L, 50mg/L, 100mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: plastic aquaria
- Material, size, headspace, fill volume: water capacity of 5 liters
- Aeration: Aeration in test vessels was provided 1 day before the start of experiment
- No. of organisms per vessel: 8 fishes
OTHER TEST CONDITIONS
- Adjustment of pH: 7.3
- Photoperiod: 12 hours light and 12-hour darkness
- TDS (ppm): 245
- Conductivity (µScm-1): 658 - Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- Determination or calculation of the median lethal concentrations (LC 50) and observations of the symptoms were done after 24, 48, 72 and 96 hours.
LC0 (96 hours) (highest loading at which no mortality was observed) = 100mg/L
LC50 (96 hours) Experimental = > 100mg/L
LC100 (96 hours) (lowest loading at which 100% mortality was observed) = 100% mortality was not observed in any of the concentrations - Sublethal observations / clinical signs:
Mortality and symptoms
Nominal Concentration
(mg/l)
Number of fish
Mortality (cumulated) after
3 h
6 h
24h
48h
72h
96h
V1
V2
V1
V2
V1
V2
V1
V2
V1
V2
0 (control)
4
4
-
-
-
-
-
-
-
-
-
-
6.25
4
4
-
-
-
-
-
-
-
-
-
-
12.5
4
4
-
-
-
-
-
-
-
-
-
-
25
4
4
-
-
-
-
-
-
-
-
-
-
50
4
4
-
-
-
-
-
-
-
-
-
-
100
4
4
-
-
-
-
-
-
-
-
-
-
Visible Symptoms:
Nominal Concentration
(mg/l)
Number of fish
Symptoms after
3 h
6 h
24h
48h
72h
96h
V1
V2
V1
V2
V1
V2
V1
V2
V1
V2
0 (control)
4
4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
6.25
4
4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
12.5
4
4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
25
4
4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
50
4
4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
100
4
4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
NS4
Explanation of symptoms:
A:apathy
N:Narcotic-like state
C:Swimming near water surface
M: Slow movements compared to control
NS:No abnormal symptoms
S:Surfacing behaviour
L:Loss of equilibrium
SM:Shimming movement
H1:Hyperactivity
H2:Hypo activity
B:Bending behavior
F:Fin rot
IS:Invert Swimming
SB:Swimming behaviour
RF:Respiratory Function
P:Pigmentation
- Validity criteria fulfilled:
- yes
- Conclusions:
- A 96 h study was conducted for CAS No. 4553-89-3 on Zebra Fish (Danio rerio), at a test concentrations of 6.25 mg/L, 12.5mg/L,25mg/L, 50mg/L, 100mg/L. The fishes were moving slowly as compared to control. No mortalities were found in the control aquaria. The lethal concentrations LC50 value was determined to be > 100mg/L.
- Executive summary:
Fish Acute Toxicity test according to OECD Guideline 203 was conducted for Disodium 4,4'-[[2,4-dihydroxy-5-(hydroxymethyl)-1,3-phenylene]bis(azo)]bisnaphthalene-1-sulphonate. The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 1 gm of the test substance in 1 liter of potable water (passed through reverse osmosis system). The nominal concentration selected for the experiment were 6.25 mg/L, 12.5mg/L, 25mg/L, 50mg/L, 100mg/L and Zebra Fish (Danio rerio) were exposed to these concentration for 96 hours. The fishes were moving slowly as compared to control. No mortalities were found in the control aquaria. The lethal concentrations LC50 value was determined to be > 100mg/L. Based on the above LC50 value it can be concluded that the target substance 4,4'-[[2,4-dihydroxy-5-(hydroxymethyl)-1,3-phenylene]bis(azo)]bisnaphthalene-1-sulphonate is non toxic to fish and thus can not be classified under Aquatic toxic category.
Reference
Description of key information
Fish Acute Toxicity test according to OECD Guideline 203 was conducted for Disodium 4,4'-[[2,4-dihydroxy-5-(hydroxymethyl)-1,3-phenylene]bis(azo)]bisnaphthalene-1-sulphonate
(UERL study report, Report no. 4553 -89 -3/01/2015/FT, Sustainability Support Services (Europe) AB has the letter of access, 2015). The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 1 gm of the test substance in 1 liter of potable water (passed through reverse osmosis system). The nominal concentration selected for the experiment were 6.25 mg/L, 12.5mg/L, 25mg/L, 50mg/L, 100mg/L and Zebra Fish (Danio rerio) were exposed to these concentration for 96 hours. The fishes were moving slowly as compared to control. No mortalities were found in the control aquaria. The lethal concentrations LC50 value was determined to be > 100mg/L. Based on the above LC50 value it can be concluded that the target substance 4,4'-[[2,4-dihydroxy-5-(hydroxymethyl)-1,3-phenylene]bis(azo)]bisnaphthalene-1-sulphonate is non toxic to fish and thus can not be classified under Aquatic toxic category.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 100 mg/L
Additional information
Data available for short term toxicity to fish endpoint of the substance Disodium 4,4’-(2,4-dihydroxy-5- hydroxymethyl-1,3-phenylene bis-azo) di- (naphthalene-1-sulfonate) are summarized as follows:
In key study, Fish Acute Toxicity test according to OECD Guideline 203 was conducted for Disodium 4,4'-[[2,4-dihydroxy-5-(hydroxymethyl)-1,3-phenylene]bis(azo)]bisnaphthalene-1-sulphonate (UERL study report, Report no. 4553 -89 -3/01/2015/FT, Sustainability Support Services (Europe) AB has the letter of access, 2015). The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 1 gm of the test substance in 1 liter of potable water (passed through reverse osmosis system). The nominal concentration selected for the experiment were 6.25 mg/L, 12.5mg/L, 25mg/L, 50mg/L, 100mg/L and Zebra Fish (Danio rerio) were exposed to these concentration for 96 hours. The fishes were moving slowly as compared to control. No mortalities were found in the control aquaria. The lethal concentrations LC50 value was determined to be > 100mg/L.
While in supporting study, Short term toxicity to fish of Disodium 4,4’-(2,4-dihydroxy-5- hydroxymethyl-1,3-phenylene bis-azo) di- (naphthalene-1-sulfonate) is estimated using QSAR Toolbox version 3.3 (2015). The Lethal concentration (LC50) of the substance Disodium 4,4’-(2,4-dihydroxy-5- hydroxymethyl-1,3-phenylene bis-azo) di- (naphthalene-1-sulfonate) in fish Oncorhynchus mykiss in a 96 hour study is estimated to be 1311.92 mg/L.
Based on the above LC50 values it can be concluded that the target substance 4,4'-[[2,4-dihydroxy-5-(hydroxymethyl)-1,3-phenylene]bis(azo)]bisnaphthalene-1-sulphonate is not likely to be toxic to fish and thus cannot be classified under Aquatic toxic category as per CLP regulation.
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