Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 203-008-4 | CAS number: 102-16-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Description of key information
Short term toxicity to aquatic invertebrates
Experimental study for inhibition of the mobility of daphnids was carried out with the benzyl phenylacetate according to OECD Guideline 202 (Report no. 003/17, Abitec 2017). The animals used for the test shall be less than 24 h old and should not be of first brood progeny. Test solutions of required concentration were prepared by mixing the stock solution of the test sample with reconstituted test water. The test substance was tested at the concentration of 0, 5, 10, 20, 40, 80 mg/L. The test was performed under static conditions in fresh water system at 20±1°C temperature. Effects on immobilisation were observed for 48 hours. EC50 was calculated using non linear regression by the software Prism 4.0.The median effective concentration (EC50) for the test substance benzyl phenylacetate(102-16-9) in Daphnia magna was determined to be 60.7 mg/L for immobilisation effects.
Based on the value benzyl phenylacetate was considered to be toxic to aquatic invertebrates and can be classified in aquatic chronic 3 category as per the CLP regulations.
Toxicity to aquatic algae and cyanobacteria
Freshwater algal growth inhibition test was carried out on Desmodesmus subspicatus with the substance Benzyl phenylacetate (CAS no. 102 -16 -9) to OECD Guideline 201 (ABITEC study report, Sustainability Support Services (Europe) AB, 2017). The stock solution (100 mg/L) was prepared by dissolving colourless liquid in acetone. Test solutions of required concentrations were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture. Conc. of test chemical used for the study was 0, 6.25, 12.5, 25, 50 and 100 mg/L, respectively. The test was performed under static conditions in a static fresh water system at a temp. of 23±2°C. Initial cell density of test organism used was 5x10(3) cells/ml. Determination of cell counting involve the use of microscope with counting chamber Cyrus I or electronic particle counter. ErC50 was calculated using non-linear regression by the software Prism 4.0. The median effective concentration (EC50) for the test substance Benzyl phenylacetate in Desmodesmus subspicatus was determined to be 34.5 mg/L on the basis of effects on growth rate in a 72 hour study. Thus, based on this value, test chemical Benzyl phenylacetate can be considered as toxic to aquatic organisms and thus can be classified as aquatic chronic category 3 as per the CLP criteria.
Toxicity to microorganisms
The Minimum Inhibitory Concentration of Corynebacterium minutissimum (CM),Arthrobacter sp. isolated from Lipo-66,Staphylococ cus aureus (IAM-1011, (SA)),Staphylococcus epidermidis var. (SE) and Escherichia coli (ATCC 11775, (EC)) micro organisms species was found to be >2000 mg/l (inoculum 105CFU/plate) after 24 hours exposure to benzyl phenylacetate.
Additional information
Short term toxicity to aquatic invertebrates
Experimental study for inhibition of the mobility of daphnids was carried out with the benzyl phenylacetate according to OECD Guideline 202 (Report no. 003/17, Abitec 2017). The animals used for the test shall be less than 24 h old and should not be of first brood progeny. Test solutions of required concentration were prepared by mixing the stock solution of the test sample with reconstituted test water. The test substance was tested at the concentration of 0, 5, 10, 20, 40, 80 mg/L. The test was performed under static conditions in fresh water system at 20±1°C temperature. Effects on immobilisation were observed for 48 hours. EC50 was calculated using non linear regression by the software Prism 4.0.The median effective concentration (EC50) for the test substance benzyl phenylacetate(102-16-9) in Daphnia magna was determined to be 60.7 mg/L for immobilisation effects.
Based on the value benzyl phenylacetate was considered to be toxic to aquatic invertebrates and can be classified in aquatic chronic 3 category as per the CLP regulations.
Toxicity to aquatic algae and cyanobacteria
Experimental key studyfor the target chemical Benzyl phenylacetate(CAS no. 102-16-9) which is supported further by experimental study for its closest structurally similar read across substance with logKow as the primary descriptor were reviewed for the toxicity to aquatic algae and cyanobacteria end point which are summarized as below:
In an experimental key study,freshwater algal growth inhibition test was carried out on Desmodesmus subspicatus with the test substance Benzyl phenylacetate (CAS no. 102 -16 -9) according to OECD Guideline 201(Alga, Growth Inhibition Test) (ABITEC study report, Sustainability Support Services (Europe) AB, 2017). The stock solution (100 mg/L) was prepared by dissolving colourless liquid in acetone. Test solutions of required concentrations were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture. Conc. of test chemical used for the study was 0, 6.25, 12.5, 25, 50 and 100 mg/L, respectively. The test was performed under static conditions in a static fresh water system at a temp. of 23±2°C. Initial cell density of test organism used was 5x10(3) cells/ml. Determination of cell counting involve the use of microscope with counting chamber Cyrus I or electronic particle counter. ErC50 was calculated using non-linear regression by the software Prism 4.0. The median effective concentration (EC50) for the test substance Benzyl phenylacetate in Desmodesmus subspicatus was determined to be 34.5 mg/L on the basis of effects on growth rate in a 72 hour study. Thus, based on this value, test chemical Benzyl phenylacetate can be considered as toxic to aquatic organisms and thus can be classified as aquatic chronic category 3 as per the CLP criteria.
In a supporting study of read across substance 2-Phenethyl phenylacetate (CAS no. 102-20-5), the effect of read across substance 2-Phenethyl phenylacetate, CAS No. 102-20-5 was studied on the growth of fresh water green alga Chlorella vulgaris (UERL Study report, Sustainability Support Services (Europe) AB, 2015). The study was conducted following OECD guideline 201- Alga growth inhibition test. The test concentration chosen for the study were 2 mg/l, 3 mg/l, 4.5 mg/l, 6.75 mg/l, 10.13 mg/l and 15.19 mg/l were prepared using stock solution of the test substance using de-ionized water. The green alga was exposed to the test concentration for a period of 72 hours to observe average specific growth rate and % growth inhibition under the effect of test substance. EC50 calculated graphically through probit analysis was observed to be 13.89 mg/l. Thus, based on this value, test chemical 2-Phenethyl phenylacetate can be considered as toxic to aquatic organisms and thus can be classified as aquatic chronic category 3 as per the CLP criteria.
Thus, based on the overall reported results for target chemical Benzyl phenylacetate (ABITEC study report, 2017) and for its read across substance (UERL study report, 2015), it can be concluded that the test substanceBenzyl phenylacetatecan be considered astoxic to aquatic organisms at environmentally relevant concentrations and can be considered to be classified inaquatic chronic category 3as per the CLP classification criteria.
Toxicity to microorganisms
Muller Hinton agar medium in culture dishes (35*10mm) was used for the measurement of MIC. Various concentrations of fragrance materials were prepared in ethyl alcohol or DMSO depending on the solubility of the materials. The bacteria tested were pre-propagated with sensitivity test broth of NISSUI using shaking culture. The incubated mediums were diluted by 0.75% physiological saline to the microbial concentration of 106CFU/ml. In the Muller Hinton agar medium containing fragrance material, 0.1ml of diluted culture solution was inoculated. MIC was determined as the concentration where no growth was observed after 24hrs at 37°C.
The Minimum Inhibitory Concentration of Corynebacterium minutissimum (CM),Arthrobacter sp. isolated from Lipo-66,Staphylococ cus aureus (IAM-1011, (SA)),Staphylococcus epidermidis var. (SE) and Escherichia coli (ATCC 11775, (EC)) micro organisms species was found to be >2000 mg/l (inoculum 105CFU/plate) after 24 hours exposure to benzyl phenylacetate.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.