Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 215-833-7 | CAS number: 1423-46-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
Description of key information
The toxicity of the test substance to aquatic algae was assessed according to OECD Test Guideline 201. The 72 hour EC50 based on growth rate was 3.0 mg/L with 95 % confidence limits of 2.8 – 3.2 mg/L. The EC10 was 1.1 mg/L, the NOEC was 0.45 mg/L and the LOEC was 1.1 mg/L.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 3 mg/L
- EC10 or NOEC for freshwater algae:
- 1.1 mg/L
Additional information
A study was performed to assess the effect of the test item on the growth of the green alga Pseudokirchneriella subcapitata. The method followed that described in the OECD Guidelines for Testing of Chemicals (2006) No 201, "Freshwater Alga and Cyanobacteria, Growth Inhibition Test" referenced as Method C.3 of Commission Regulation (EC) No 761/2009.
Preliminary solubility work conducted indicated that it was not possible to obtain a testable solution of the test item using traditional methods of preparation e.g. ultrasonication.
A preliminary media preparation trial indicated that a dissolved test item concentration of approximately 85 mg/L was obtained from a saturated solution method of preparation indicating this to be the limit of water solubility of this item under test conditions.
Following a preliminary range-finding test, Pseudokirchneriella
subcapitata was exposed to solutions of the test item at nominal
concentrations of 2.6, 6.4, 16, 40 and 100% v/v saturated solution
(three replicate flasks per concentration) for 72 hours, under constant
illumination and shaking at a temperature of 24 ± 1 °C. The test item
solutions were prepared by stirring an excess (100 mg/L) of test item in
culture medium using a propeller stirrer at approximately
1500 rpm for 24 hours. After the stirring period any undissolved test
item was removed by filtration (0.2 µm Gelman Acrocap filter, first
approximate 500 mL discarded in order to pre-condition the filter) to
produce a 100% v/v saturated solution of the test item. This saturated
solution was then further diluted as necessary, to provide the remaining
test groups.
Samples of the algal populations were removed daily and cell concentrations determined for each control and treatment group, using a Coulter®Multisizer Particle Counter.
Analysis of the test preparations at 0 hours showed measured test concentrations to range from 0.30 to 50 mg/L. Measured concentrations at 72 hours were in the range of 0.68 to 46 mg/L. Analysis of samples at 72 hours prepared with the omission of algal cells and incubated alongside the test showed measured test concentrations to range from 0.50 to 44 mg/L. Given this it was considered appropriate to calculate the results based on the geometric mean measured test concentrations only.
Exposure of Pseudokirchneriella subcapitata to the test item gave the following results based on the geometric mean measured test concentrations:
The 72 hour EC50 based on growth rate was 3.0 mg/L with 95 % confidence limits of 2.8 – 3.2 mg/L. The corresponding EC10 was 1.1 mg/L, the NOEC was 1.1 mg/L and the LOEC was 3.0 mg/L.
The preferred observational end point in this study is algal growth rate inhibition because it is not dependent on the test design (ECHA guidance Chapter R.7b v1.1). The EU CLP regulation (No 1272/2008 and its adaption 286/2011) also states that classification should be based on the ErC50. The preferred observational endpoint in long-term studies is the EC10 value because it is derived from the dose response curve. In contrast the NOEC depends on the experiment design (e.g. the concentrations used in the test). Thus the 72-h EC50 and EC10 based on growth rate are used for classification purposes.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.