Registration Dossier
Registration Dossier
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EC number: 207-434-1 | CAS number: 471-01-2
Specific investigations: other studies
Administrative data
- Endpoint:
- biochemical or cellular interactions
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Study well documented, meets generally accepted scientific principles, acceptable for assessment.
Data source
Reference
- Reference Type:
- publication
- Title:
- Adrenal-dependent leucopenia after short-term exposure to various airborne irritants in rats
- Author:
- Brondeau MT, Bonnet P, Guenier JP, Simon P, de Ceaurriz J
- Year:
- 1 990
- Bibliographic source:
- J Appl Toxicol 10(2): 83-86
Materials and methods
Test guideline
- Qualifier:
- no guideline required
- GLP compliance:
- not specified
- Type of method:
- in vivo
- Endpoint addressed:
- other: blood cell count
Test material
- Reference substance name:
- 3,5,5-trimethylcyclohex-2-enone
- EC Number:
- 201-126-0
- EC Name:
- 3,5,5-trimethylcyclohex-2-enone
- Cas Number:
- 78-59-1
- IUPAC Name:
- 3,5,5-trimethylcyclohex-2-en-1-one
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Sprague-Dawley
- Sex:
- male
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Weight at study initiation: 250-300 g
- Diet: free access, except during exposure
- Water: free access, except during exposure
- Acclimation period: at least 5 days
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22
- Photoperiod (hrs dark / hrs light): 12 / 12
Administration / exposure
- Route of administration:
- inhalation: vapour
- Vehicle:
- unchanged (no vehicle)
- Analytical verification of doses or concentrations:
- yes
- Duration of treatment / exposure:
- 4 hours
- Frequency of treatment:
- once
Doses / concentrations
- Remarks:
- Doses / Concentrations:
19, 49, 67 and 90 ppm (corresponds to 0.107, 0.276, 0.378 and 0.508 mg/L)
Basis:
- No. of animals per sex per dose:
- 5
- Control animals:
- not specified
- Details on study design:
- At the end of the exposure period, rats were anaesthetized with pentobarbital and exsanguinated from the abdominal aorta.
Examinations
- Examinations:
- Blood samples were collected in tubes containing complexon as an anticoagulant.
Red blood cells, total white blood cell and leukocyte differential counts were performed.
Results and discussion
- Details on results:
- A statistically significant decrease in the number of circulating leukocytes (without any change in differential or red blood cell counts) was observed in rats exposed for 4 hours to increasing concentrations of isophorone.
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
