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EC number: 690-512-6 | CAS number: 1271172-98-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vitro / ex vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Study conducted to OECD guidelines and GLP
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 011
- Report date:
- 2011
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 437 (Bovine Corneal Opacity and Permeability Test Method for Identifying Ocular Corrosives and Severe Irritants)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Reaction products of 1,3,5-triazine-2,4,6-triamine and zinc bis(dihydrogen phosphate)
- EC Number:
- 690-512-6
- Cas Number:
- 1271172-98-5
- Molecular formula:
- Zn(C3H7N6)2(P2O7)
- IUPAC Name:
- Reaction products of 1,3,5-triazine-2,4,6-triamine and zinc bis(dihydrogen phosphate)
- Test material form:
- solid: particulate/powder
- Remarks:
- migrated information: powder
Constituent 1
Test animals / tissue source
- Species:
- other: Bovine eyes
- Details on test animals or tissues and environmental conditions:
- Preparation of corneas:
Fresh bovine eyes were obtained from the slaughterhouse on the day of the test. They eyes were transported in Hank's balanced salt solution. The corneas were dissected and incubated with with media at 32 +/- 1 °C in an incubation chamber for 1 hour. The corneas were examined and only those free from defects were used. The corneas were excised with a scalpel and cut from the globe with a 2-3 mm ring of sclera around the outside.
After the corneal holders were cleaned and sterilised, they were kept in an incubation chamber at 32°C. On the day of the assay, the minimum essential medium (MEM) without Phenol red was supplemented with sodium bicarbonate, L-glutamine and 1% fetal calf serum and stored in a water bath at 32°C. The same was performed for the MEM with phenol red. Each cornea was transferred to a corneal holder in which pre-warmed cMEM without phenol red was filled. The holders were then incubated for 1 hour at 32°C.
Test system
- Vehicle:
- unchanged (no vehicle)
- Remarks:
- tested neat on the corneal surfaces
- Controls:
- yes
- Amount / concentration applied:
- On average, 231.4 mg of the test substance was applied directly on the corneal surface (each cornea) using a weight board. The test item was given on the epithelium in such a manner that as much as possible of the cornea was covered with the test item.
- Duration of treatment / exposure:
- 4 hours (+/- 5 minutes) at 32°C.
- Number of animals or in vitro replicates:
- Three eyes for the test substance, negative controls and positive control substances.
- Details on study design:
- After the initial incubation, the medium was changed and the baseline opacity was measured. This was performed by placing the holder with the cornea in a spectrophotometer and recording the absorption at 570 nm.
Results and discussion
In vitro
Results
- Irritation parameter:
- in vitro irritation score
- Remarks:
- mean value
- Value:
- 0.016
- Negative controls validity:
- valid
- Positive controls validity:
- valid
In vivo
- Irritant / corrosive response data:
- The individual in vitro irritancy scores for the negative controls were 0.2703 to 0.9344. The individual positive control in vitro irritancy scores ranged from 67.8 to 83.6.
The corneas treated with the test substance showed opacity values of 0.38 to 1.2 and permeability values ranging from 0.0035 to 0.02. Hence, the in vitro irritancy scores ranged from -0.2 to 0.7 after 4 hours of treatment (mean 0.0160). - Other effects:
- The positive control substance induced an IVIS score of 75.9 (showing severe eye irritancy) and indicating the test was valid.
Any other information on results incl. tables
IVIS values:
Test Group |
IVIS |
Mean IVIS |
Negative control (0.9% saline) |
0.9344 |
0.5930 |
0.2703 |
||
0.5748 |
||
Test substance |
-0.3112 |
0.0160 |
-0.2993 |
||
0.6598 |
||
Positive control (20% imidazole) |
67.7997 |
75.8590 |
76.1348 |
||
83.6427 |
Applicant's summary and conclusion
- Interpretation of results:
- not irritating
- Remarks:
- Migrated information Criteria used for interpretation of results: EU
- Conclusions:
- Since the test substance induced an IVIS ≤ 3, no classification is required for eye irritation or serious eye damage.
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