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EC number: 225-897-8 | CAS number: 5137-70-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- between 18 March 1997 and 7 April 1997
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Remarks:
- Guideline study to GLP but TA102 or E.Coli were not tested.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 997
- Report date:
- 1997
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- yes
- Remarks:
- : TA 102 or E.coli were not tested
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.13/14 (Mutagenicity - Reverse Mutation Test Using Bacteria)
- Deviations:
- not specified
- Principles of method if other than guideline:
- Not applicable.
- GLP compliance:
- yes (incl. QA statement)
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Octylphosphonic acid
- EC Number:
- 225-218-5
- EC Name:
- Octylphosphonic acid
- Cas Number:
- 4724-48-5
- Molecular formula:
- C8H19O3P
- IUPAC Name:
- octylphosphonic acid
- Test material form:
- other: solid
- Details on test material:
- - Name of test material (as cited in study report): Octylphosphonic Acid (with no residual ester):
Constituent 1
Method
- Target gene:
- Histidine for Salmonella
Species / strain
- Species / strain / cell type:
- other: S. typhimurium strains TA1535, TA1537, TA1538, TA98, TA100
- Details on mammalian cell type (if applicable):
- Not applicable
- Metabolic activation:
- with and without
- Metabolic activation system:
- Rat S9 mix
- Test concentrations with justification for top dose:
- 5 to 5000 µg/plate
- Vehicle / solvent:
- acetone
Controlsopen allclose all
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- Remarks:
- acetone
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: 2-aminoanthracene (2AA)
- Remarks:
- for all strains with metabolic activation
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- Remarks:
- acetone
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- N-ethyl-N-nitro-N-nitrosoguanidine
- Remarks:
- for TA100 and TA1535 without metabolic activation
Migrated to IUCLID6: ENNG
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- Remarks:
- acetone
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- Remarks:
- for TA1537 without metabolic activation
Migrated to IUCLID6: 9AA
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- Remarks:
- acetone
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: 4-Nitro-o-phenylenediamine (4NOPD)
- Remarks:
- for TA1538 without metabolic activation
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- Remarks:
- acetone
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- 4-nitroquinoline-N-oxide
- Remarks:
- for TA98 without metabolic activation
Migrated to IUCLID6: 4NQO
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in agar (plate incorporation)
DURATION
- Preincubation period: no
- Exposure duration: 48h at 37°C
NUMBER OF REPLICATIONS: 3
DETERMINATION OF CYTOTOXICITY
- Method: the plates were assessed for revertant colonies using a Domino colony counter and examined for a thinning of the background lawn. - Evaluation criteria:
- For a substance to be considered positive in this test system, it should have induced a dose-related and statistically significant increase in mutation rate in one or more strains of bacteria in the presence and/or absence of the S9 microsomal enzymes in both experiments at sub-toxic dose levels. If the experiments give conflicting results, a third experiment may be used to confirm the correct result.
To be considered negative, the number of induced revertants compared to spontaneous retertants should be less than twofold at each dose level, the intervals of which should be between 2 and 5 fold and extend to the limits imposed by toxicity, solubility or up to the maximum recommended dose of 5000 µg/plate. - Statistics:
- All data are statistically analysed using the methods recommended by the UKEMS and normally Dunnett’s method of linear regression is used to evaluate the result.
Results and discussion
Test results
- Species / strain:
- other: S. typhimurium TA1535, TA1537, TA1538, TA98, TA100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- from 1500 ug/plate
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Additional information on results:
- TEST-SPECIFIC CONFOUNDING FACTORS: no data
RANGE-FINDING/SCREENING STUDIES:
Due to the acidic nature of the test material, the preliminary toxicity assay was performed with TA100 both with and without S9-mix and with a dose range of 5 to 5000 µg/plate. The test material exhibited toxicity at and above 1500 µg/plate.
COMPARISON WITH HISTORICAL CONTROL DATA: no data
ADDITIONAL INFORMATION ON CYTOTOXICITY:
The test material caused a visible reduction in the growth of the bacterial background lawn to all of the tester strains, both with and without metabolic activation. The first indication of a toxic response was observed at 1500 µg/plate. - Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
Experiment 1
Without activation
Test Group |
Dose Level (µg/plate) |
Revertant Colony Counts |
|
|||||||||
|
|
TA 1535 |
TA 1537 |
TA 1538 |
TA 98 |
TA100 |
||||||
|
|
mean |
SD |
mean |
SD |
mean |
SD |
mean |
SD |
mean |
SD |
|
DMSO |
Vehicle control |
24 |
3.6 |
10 |
2.9 |
32 |
3.5 |
28 |
7.1 |
116 |
5.5 |
|
Test |
5 |
27 |
5.5 |
9 |
3.8 |
36 |
1.0 |
27 |
1.7 |
112 |
1.5 |
|
|
15 |
30 |
1.0 |
10 |
1.7 |
34 |
3.1 |
23 |
3.8 |
113 |
6.8 |
|
|
50 |
29 |
2.1 |
7 |
3.5 |
36 |
2.5 |
19 |
5.8 |
117 |
13.6 |
|
|
150 |
29 |
3.6 |
6 |
1.2 |
29 |
6.5 |
24 |
2.0 |
123 |
4.6 |
|
|
500 |
27 |
4.4 |
5 |
1.0 |
27 |
1.0 |
20 |
6.2 |
103 |
9.9 |
|
|
1500 |
24 |
5.5 |
4 |
2.5 |
23 |
4.9 |
20 |
3.6 |
0 |
0.0 |
|
|
5000 |
15 |
4.0 |
3 |
0.6 |
0 |
0.0 |
13 |
2.1 |
0 |
0.0 |
|
ENNG |
5.0 |
208 |
18.9 |
|
|
|
|
|
|
|
|
|
|
3.0 |
|
|
|
|
|
|
|
|
476 |
47.0 |
|
9AA |
80 |
|
|
742 |
19.5 |
|
|
|
|
|
|
|
4NOPD |
5.0 |
|
|
|
|
680 |
90.1 |
|
|
|
|
|
4NQO |
0.2 |
|
|
|
|
|
|
376 |
66.8 |
|
|
|
With activation
Test Group |
Dose Level (µg/plate) |
Revertant Colony Counts |
|
||||||||||
|
|
TA 1535 |
TA 1537 |
TA 1538 |
TA 98 |
TA100 |
|||||||
|
|
mean |
SD |
mean |
SD |
mean |
SD |
Mean |
SD |
mean |
SD |
||
DMSO |
Vehicle control |
20 |
4.6 |
12 |
1.0 |
36 |
3.5 |
32 |
2.1 |
115 |
2.6 |
||
Test |
5 |
19 |
2.6 |
15 |
2.3 |
33 |
0.0 |
34 |
4.5 |
105 |
11.9 |
||
|
15 |
16 |
0.6 |
11 |
2.3 |
28 |
1.5 |
27 |
5.7 |
130 |
8.1 |
||
|
50 |
22 |
2.0 |
11 |
0.0 |
32 |
1.5 |
29 |
6.0 |
120 |
14.8 |
||
|
150 |
23 |
5.0 |
13 |
1.5 |
32 |
2.3 |
26 |
3.2 |
123 |
3.2 |
||
|
500 |
19 |
3.1 |
11 |
1.5 |
33 |
6.0 |
31 |
3.8 |
115 |
8.4 |
||
|
1500 |
9 |
2.1 |
11 |
1.2 |
34 |
5.7 |
19 |
4.9 |
14 |
2.5 |
||
|
5000 |
8 |
4.2 |
6 |
0.6 |
2 |
1.2 |
5 |
1.0 |
0 |
0.0 |
||
2AA |
2.0 |
244 |
17.7 |
257 |
71.7 |
|
|
|
|
|
|
||
|
1.0 |
|
|
|
|
|
|
|
|
845 |
74.3 |
||
|
0.5 |
|
|
|
|
237 |
5.5 |
304 |
37.3 |
|
|
||
Experiment 2
Without activation
Test Group |
Dose Level (µg/plate) |
Revertant Colony Counts |
|
|||||||||
|
|
TA 1535 |
TA 1537 |
TA 1538 |
TA 98 |
TA100 |
||||||
|
|
mean |
SD |
mean |
SD |
mean |
SD |
mean |
SD |
mean |
SD |
|
DMSO |
Vehicle control |
23 |
4.7 |
9 |
2.6 |
30 |
6.0 |
22 |
5.3 |
116 |
4.9 |
|
Test |
15 |
|
|
|
|
34 |
9.9 |
|
|
114 |
2.6 |
|
|
50 |
22 |
9.2 |
9 |
0.0 |
33 |
5.5 |
20 |
2.0 |
116 |
6.9 |
|
|
150 |
25 |
6.0 |
10 |
2.1 |
28 |
3.6 |
26 |
6.7 |
116 |
6.0 |
|
|
500 |
26 |
5.9 |
7 |
1.5 |
25 |
6.6 |
20 |
4.4 |
118 |
12.7 |
|
|
1500 |
22 |
7.6 |
7 |
1.5 |
10 |
1.2 |
18 |
2.6 |
0 |
0.0 |
|
|
5000 |
13 |
3.2 |
3 |
0.6 |
0 |
0.0 |
7 |
4.2 |
0 |
0.0 |
|
ENNG |
5.0 |
290 |
42.9 |
|
|
|
|
|
|
|
|
|
|
3.0 |
|
|
|
|
|
|
|
|
541 |
18.5 |
|
9AA |
80 |
|
|
711 |
112.7 |
|
|
|
|
|
|
|
4NOPD |
5.0 |
|
|
|
|
561 |
7.0 |
|
|
|
|
|
4NQO |
0.2 |
|
|
|
|
|
|
186 |
8.2 |
|
|
|
With activation
Test Group |
Dose Level (µg/plate) |
Revertant Colony Counts |
|
|||||||||
|
|
TA 1535 |
TA 1537 |
TA 1538 |
TA 98 |
TA100 |
||||||
|
|
mean |
SD |
mean |
SD |
mean |
SD |
Mean |
SD |
mean |
SD |
|
DMSO |
Vehicle control |
17 |
4.6 |
8 |
3.8 |
22 |
3.2 |
24 |
3.1 |
140 |
15.3 |
|
Test |
15 |
|
|
|
|
|
|
|
|
131 |
12.5 |
|
|
50 |
15 |
3,6 |
10 |
1.5 |
24 |
2.3 |
24 |
5.8 |
155 |
20.0 |
|
|
150 |
16 |
2.9 |
9 |
1.7 |
23 |
3.1 |
28 |
9.2 |
148 |
8.6 |
|
|
500 |
20 |
1.5 |
6 |
0.6 |
20 |
7.5 |
30 |
4.5 |
146 |
21.5 |
|
|
1500 |
13 |
1.7 |
9 |
1.1 |
22 |
6.0 |
21 |
5.5 |
13 |
1.2 |
|
|
5000 |
11 |
3.6 |
0 |
0.0 |
0 |
0.0 |
0 |
0.0 |
0 |
0.0 |
|
2AA |
2.0 |
234 |
26.6 |
159 |
26.2 |
|
|
|
|
|
|
|
|
1.0 |
|
|
|
|
|
|
|
|
1139 |
49.1 |
|
|
0.5 |
|
|
|
|
227 |
17.2 |
224 |
25.1 |
|
|
|
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results
negative without metabolic activation
negative with metabolic activation
OPA was considered to be non-mutagenic with and without metabolic activation under the conditions of this test. - Executive summary:
Salmonella typhimurium strains TA1535, TA1537, TA98 and TA100 were treated with the test material using the Ames plate incorporation method at up to seven dose levels, in triplicate, both with and without the addition of a rat liver homogenate metabolising system (10% liver S9 in standard co-factors). The dose range was determined in a preliminary toxicity assay and was 5 to 5000 µg/plate in the first experiment. The experiment was repeated on a separate day using a dose range of 15 to 5000 µg/plate with fresh cultures of the bacterial strains and fresh test material formulations.
The vehicle (acetone) control plates produced counts of revertant colonies within the normal range.
All the positive control chemicals used in the test induced marked increases in the frequency of revertant colonies, both with and without the metabolising systems.
The test material caused a visible reduction in the growth of the bacterial background lawn to all the tester strains both with and without metabolic activation. The first indication of a toxic response was observed at 1500 µg/plate.No significant increases in the frequency of revertant colonies was recorded for any of the bacterial strains with any dose of the test material, either with or without metabolic activation. The test material was considered to be non-mutagenic under the conditions of this test.
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