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EC number: 253-410-9 | CAS number: 37224-61-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge nitrification inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 8th August 1985 - 14th August 1985.
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: see 'Remark'
- Remarks:
- The undersigned declares that this work was carried out under supervision according to the procedures herein described and according to the OECD principles of Good Laboratory Practice, and that this report represents a true and accurate record of the results obtained.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 985
- Report date:
- 1985
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: ETAd method for the Determination of the Inhibitory Effects of Chemicals and industrial Waste Waters on the Respiration and Nitrification of Activated Sludge
- GLP compliance:
- yes
Test material
- Reference substance name:
- Isooctyldinitrophenol
- EC Number:
- 253-410-9
- EC Name:
- Isooctyldinitrophenol
- Cas Number:
- 37224-61-6
- Molecular formula:
- C14H20N2O5
- IUPAC Name:
- 4-(6-methylheptyl)-3,5-dinitrophenol
- Test material form:
- liquid: viscous
- Details on test material:
- Test substance was received from Thomas Swan and Co. Ltd., Crookhall, Consett, Durham, DH8 7ND on 30th May 1985. Substance was a brown, viscous, liquid contained in a white opaque plastic bottle with a screw cap.
Inclusive weight of the test substance and container on receipt was 492.03g. The test substance was stored under ambient temperature until required.
Chemical name: Dinitro Capryl Phenol
Other names: Dinitro octyl phenol
Molecular formula: C14H20N2O5
Chemical composition: DNCP - 89.9%, MNOP - 4.6%, NDOP - 4.0%, TNP - 1.0%
Appearance, colour: Dark red
Solubility in water: 30mg/l
Specific gravity: 1.13
Stability: stable, instability caused by overheating
Intended use: Fungicide
Analysis report for the sample of D.N.C.P supplied for use in this test by the sponser is as follows:
DNCP P511 B68/85
% H20 - 0.08
% DNCP - 89.9
% MNCP - 4.55
% strong acid as Picric Acid - 0.53
Total titratable phenols (meq/grm) - 3.24
Constituent 1
Test solutions
- Details on test solutions:
- Reference Inhibitor: 3,5-Dichlorophenol (3,5-DCP)
Nitrification Inhibitor: Allyl Thiourea (ATU)
Test organisms
- Test organisms (species):
- activated sludge
- Details on inoculum:
- Synthetic sewage:
A 100-fold concentrated solution of OECD/EEC synthetic sewage was prepared by dissolving the following in one litre of deionised water:
27.00g 'Lan-Lemco' Broth
3.00g Urea
2.80g Dipotassium hydrogen orthphosphate
0.70g Sodium Chloride
0.40g Calcium chloride dihydrate
0.20g Magnesium sulphate heptahydrate
The solution was kept ina refrigerator at 4°C until required.
Study design
- Test type:
- not specified
- Water media type:
- not specified
- Total exposure duration:
- 3 h
Test conditions
- Test temperature:
- Preliminary Test: 22°C
Definitive Test: 22°C - Nominal and measured concentrations:
- nominal: 100mg/l
Results and discussion
Effect concentrations
- Duration:
- 1 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 30 - < 137.1 mg/L
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of nitrification rate
- Remarks on result:
- other: 95% CL
- Details on results:
- Preliminary test
The concentration of suspended solids in the sample of activated sludge collected for the preliminary test was estimated to be 4944 mg/l. 3034 ml of sludge was made up to 5000ml by the addition of groundwater to produce a sludge with a nominal concentration of suspended solids of 3000 mg/l.
The results of the preliminary test are shown in table 1. There were no ihibitory effects on oxygen consumption at nominal test substance concentrations of 1 and 10mg/l. However, there was evidence to suggest that the test substance stimulated oxygen consumption at these concentrations (Bottles 12 and 14). A 52% inhibition was noted at a nominal test substance concentration of 100mg/l (Bottle 16).
In bottles 13, 15 and 17 containing the test substance and ATU, oxygen consumption decreased with increasing conc. of the test substance. The maximum inhibition observed in these mixtures (69%, 100 mg/l DNCP) did not exceed that in the control + ATU mixtures (50% to 70%, Bottles 2 an 11).
The EC50 of the test substance to bacteria using oxygen in the sludge was estimated to be approx. 100 mg/l.
Oxygen was not consumed by the abiotic control mixture.
The EC50 of the reference inhibitor, 3,5-DCP was estimated to be between 1 and 10mg/l. A 100% inhibition of oxygen consumption was noted in the mixture containing 100mg/l of 3,5-DCP + ATU.
Definitive Test
The concentration of suspended solids in the sample of activated sludge collected fpr the second test was estimated to be 4548mg/l. 3300ml of sludge were made up to 5000 mg/l by the addition of groundwater to produce a sludge with a nominal concentration of suspended solids of 3000 mg/l.
The results of the definitive test are shown in table 2. inhibtion of oxygen consumption was observed at each of the three test substance concentrations tested (50, 100 and 150 mg/l). The mean percentage inhibition at each concentration was 5%, 29& and 42% respectively. The EC50 of the test substance to bacteria using oxygen in the sludge was estimated to be >150mg/l.
As with the preliminary test, the maximum inhibition by the test substance (mean of 42% at 150 mg/l) did not exceed that observed in the control + ATU mixture (mean of 44%).
the EC50 of the test substance to bacteria involved in nitrification was estimated to be 91.5mg/l. The 95% confidence limits were calculated to be 30.1 mg/l (lower limit) and 137.1 mg/l (upper limit)
100mg/l of 3,5-DCP inhibited oxygen consumption by approx. 96%.
Any other information on results incl. tables
Table 1: Preliminary test: Rates of oxygen consumption of each test mixture with % inhibtion by the test substance, 3,5 -dochlorophenol and Allyl thiourea.
Bottle No. | Bottle Contents | Rate of O2 consumption (mg O2/l/hr) | % inhibition |
1 | Control | 36.1 | - |
9 | Control | 23.5 | - |
10 | Control | 38.3 | - |
Mean | 32.7 | - | |
3 | 1 mg/l 3,5 -DCP | 21.6 | 34 |
5 | 10mg/l 3,5 -DCP | 4.2 | 87 |
7 | 100mg/l 3,5 -DCP | 3.9 | 88 |
12 | 1mg/l DNCP | 40.9 | -25 |
14 | 10mg/l DNCP | 40.6 | -24 |
16 | 100mg/l DNCP | 15.7 | 52 |
2 | Control + ATU | 9.1 | 72 |
11 | Control + ATU | 15.7 | 52 |
4 | 1mg/l 3,5 -DCP + ATU | 7.9 | 76 |
6 | 10mg/l 3,5 -DCP + ATU | 8.5 | 74 |
8 | 100mg/l 3,5 -DCP + ATU | 0 | 100 |
13 | 1mg/l DNCP + ATU | 18.5 | 43 |
15 | 10mg/l DNCP + ATU | 14.1 | 57 |
17 | 100mg/l DNCP + ATU | 10.2 | 69 |
18 | 100mg/l DNCP (Abiotic control) | 0 | 100 |
Table 2: Definitive test: Rates of oxygen consumption of each test mixture with the % inhibition by the test substance, 3,5 -Dichlorophenol and Allyl thiourea
Bottle No. | Bottle Contents | Rate of O2 consumption (mg O2/l/hr) | % inhibition | % inhibition of Nitrification |
1 | Control | 42.1 | - | - |
7 | Control | 41.2 | - | - |
12 | Control | 41.9 | - | - |
Mean | 41.7 | |||
2 | Control + ATU | 22.5 | 46 | - |
8 | Control + ATU | 23.7 | 43 | - |
13 | Control + ATU | 23.7 | 43 | - |
Mean | 23.3 | 44 | - | |
3 | 100mg/l 3,5 -DCP | 1.7 | 96 | - |
4 | 50mg/l DNCP | 34.2 | 18 | 41 |
11 | 50mg/l DNCP | 41.1 | 1 | 1 |
15 | 50mg/l DNCP | 44.0 | -5 | -12 |
Mean | 39.8 | 5 | 10 | |
5 | 100mg/l DNCP | 25.9 | 38 | 83 |
9 | 100mg/l DNCP |
23.7 | 43 | 100 |
16 | 100mg/l DNCP | 39.5 | 5 | 13 |
Mean | 39.8 | |||
6 | 150mg/l DNCP | 17.0 | 59 | 128 |
10 | 150mg/l DNCP | 21.9 | 47 | 110 |
14 | 150mg/l DNCP | 33.3 | 20 | 47 |
Mean | 24.1 | 42 | 96 |
Applicant's summary and conclusion
- Conclusions:
- The test substance was found not to inhibit oxygen consumption by activated sludge at nominal test substance concentrations of 50, 100 and 150 mg/l. In both the preliminary and definitive test the extent of this inhibtion did not exceed that caused by the ATU (the known inhibitor of nitrification). Therefore, it would seem likely that the test substance was inhibitory to to nitrification and not carbonaceous respiration at the concentrations tested. In view of this finding the percentage inhibition of nitrification by the test substance in mixtures prepared in the definitive test was calculated. The EC50 of DNCP to bacteria involved in nitrification was estimated to be 91.5mg/l. This value had a lower 95% confidence limit of 30.0mg/l and an upper limit of 137.1mg/l.
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