Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
Reaction mass of copper complex of [(2,6-difluoroheterocycl-4-yl)amino]hydroxy{[2-hydroxy-3-sulfonato-5-(vinylsulfonyl)phenyl]diazenyl}naphthalene sulfonic acid, dialkali salt and copper complex of [(2,6-difluoroheterocycl-4-yl)amino]-hydroxy{[2-hydroxy-3-sulfonato-5-{[2-(sulfonatooxy)ethyl]sulfonyl}phenyl]diazenyl}naphthalene sulfonic acid, trialkali salt
EC number: 479-550-2 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
Description of key information
The reduction in biomass gain and growth rate in this test was the result of light absorption effects of the test substance instead of a toxic effect. Consequently, the results from this test are not appropriate to evaluate the toxicity of the test substance for algae. The EC50 value (in terms of chemical toxicity) could not be determined, but the hypothetical EC50 and NOEC values are likely to be substantially higher than the limit test concentration of 100 mg/L.
Key value for chemical safety assessment
Additional information
A study was performed to assess adverse effects of the test substance on the growth (= increase in cell density) and the growth rate (= rate of increase in cell density with time) of the planktonic freshwater algal species Desmodesmus subspicatus over several generations. The study was conducted in accordance with EU method C.3 'Algal inhibition test' which is in most parts equivalent to the OECD guideline 201 'Alga, Growth Inhibition Test'. The test included a modification to enable determination of light attenuation by coloured test substances as a possible cause of growth inhibition as published by Memmert & Knoell (RCC Umweltchemie, August 1992).
Exponentially growing algal cells were exposed to a range of test substance concentrations for a period of 72 hours, nominally 0.62, 1.4, 3.0, 6.6, 14.5, 32 and 70.4 mg/l of test substance dissolved in water (PART I). A second series (PART II) of test vessels was treated in the same way as the controls, but placed below glass vessels containing the same range of test item concentrations as described above, but without algae. This test design allowed to distinguish between algistatic (indirect effect caused by light absorption) and algicidal (toxic) effects.
The cell densities were measured at 24 hour intervals. Inhibition of the algal population was measured as reduction in growth (index b) and growth rate (index r), relative to control cultures grown under identical conditions. Growth and growth rates were used to calculate a 'No Observed Effect Concentration' and a 'Lowest Observed Effect Concentration' according to DUNNETT (1955, 1964).
All results are expressed in terms of nominal concentrations. Measured concentrations ranged from 101 to 113 % of nominal values at 0 hours, and from 99 to 110 % of nominal values at 72 hours.
There was only little difference in growth inhibition between trials exposing algae directly to the test substance and trials using the test substance as a light filter only (test vessels with algae covered with glass dishes containing test item solution). For the nominal concentrations 14.5 and 32 mg/l differences of 10.1 and 18.4 % were determined, respectively. The concentrations above (70.4 mg/l) and below (6.6 mg/l) resulted in 0.6 % and 4.9 % difference, respectively. Accordingly, no concentration-response relationship demonstrating a true algicidal effect could be established. Therefore, the inhibition of growth observed in this study appeared to be caused by light absorption only. Consequently, the effect concentrations from this test are not appropriate for evaluating the toxicity of the test substance to algae.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.