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EC number: 942-932-9 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 979
- Report date:
- 1979
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- yes
- Remarks:
- no E.coli strain tested
- GLP compliance:
- no
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 2-[(2-hydroperoxy-4-methylpentan-2-yl)peroxy]-4-methylpentane-2-peroxol; 4-methylpentan-2-one; 4-methylpentane-2,2-diperoxol
- EC Number:
- 942-932-9
- Cas Number:
- 37206-20-5
- Molecular formula:
- Reaction mass of C6H14O4 and C12H26O6 and C6H12O
- IUPAC Name:
- 2-[(2-hydroperoxy-4-methylpentan-2-yl)peroxy]-4-methylpentane-2-peroxol; 4-methylpentan-2-one; 4-methylpentane-2,2-diperoxol
- Reference substance name:
- Diisobutyl phthalate
- EC Number:
- 201-553-2
- EC Name:
- Diisobutyl phthalate
- Cas Number:
- 84-69-5
- Molecular formula:
- C16H22O4
- IUPAC Name:
- diisobutyl phthalate
- Test material form:
- liquid
Constituent 1
additive 1
Method
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Species / strain / cell type:
- S. typhimurium TA 1538
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 mix Aroclor 1254 induced (rat liver)
- Test concentrations with justification for top dose:
- 0, 0.0005, 0.001, 0.002, 0.1 µL test liquid/0.1 mL acetone/plate (1st test)
0, 0.0005, 0.001, 0.002, 0.01 µL test liquid/0.1 mL acetone/plate (2nd test)
0, 0.1, 0.25, 0.5, 1.0 (positive control) µg 2-AA/0.1 mL DMSO/plate - Vehicle / solvent:
- DMSO and aceton
Controls
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: 2-Aminoanthracene
- Remarks:
- All strains with and without metabolic activation
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in agar (plate incorporation)
DURATION
- Exposure duration: 3 days at 37 °C
SELECTION AGENT (mutation assays): L-Histidine
NUMBER OF REPLICATIONS: 3
Results and discussion
Test resultsopen allclose all
- Key result
- Species / strain:
- S. typhimurium TA 1535
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- not valid
- Key result
- Species / strain:
- S. typhimurium TA 1537
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- not valid
- Key result
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- not valid
- Key result
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- not valid
Any other information on results incl. tables
The addition of 2 -aminoanthracene showed a significant bacterial growth by the addition of S-9 mix, no significant bacterial growth was observed without the addition of S-9 mix. The amount of S-9 mix used was not optimal for the metabolic activation of 2 -AA. The positive control was not valid in this test.
Applicant's summary and conclusion
- Conclusions:
- The genetic toxicity of the test item was determined in an Ames test equivalent to OECD guideline 471. No genetic toxicity was observed in this study.
- Executive summary:
The mutagenic activity of the test item was tested in a study equivalent to OECD guideline 471. A set of 5 histidine requiring mutants of S. typhimurium (TA1535, TA1537, TA 1538, TA 98 and TA100) were used in the Ames-test with and without metabolic activation.
Doses of 0, 0.0005, 0.001, 0.002, 0.1 µL test liquid/0.1 mL acetone/plate (1st test) and 0, 0.0005, 0.001, 0.002, 0.01 µL test liquid/0.1 mL acetone/plate (2nd test) were tested. The strains were incubated over a period of 3 days at 37 °C. Incorporation of the test substance up to non-inhibitory levels did not increase the number of his+ revertants in any of the five tester strains, either in the presence or in the absence of the liver microsome activation system. It was concluded that the present results did not reveal mutagenic acitivity in the Salmonella mutagenicity test.
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