Registration Dossier
Registration Dossier
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EC number: 223-095-2 | CAS number: 3734-33-6
Endpoint summary
Administrative data
Description of key information
Biodegradation in water
28-days Manometric respirometry test following the OECD guideline 301F to determine the ready biodegradability of the test item Denatonium benzoate (CAS No. 3734-33-6) was conducted. The test system included control, test item and reference item. The concentration of test and reference item (Sodium Benzoate) chosen for both the study was 100mg/L, while that of inoculum was 10ml/l. ThOD (Theoretical oxygen demand) of test and reference item was determined by calculation. % Degradation was calculated using the values of BOD and ThOD for test item and reference item. The BOD28value of Denatonium benzoate(CAS No. 3734-33-6) was observed to be 0.436 mgO2/mg. ThOD was calculatedas 2.40mgO2/mg. Accordingly, the % degradation of the test item after 28 days of incubation at 20 ± 1°C according to manometric respirometry test was found to be 18.17%. Based on the results, the test item, under the test conditions, was found to be non-biodegradable at 20 ± 1°C over a period of 28 days.
Biodegradation in water and sediment
Estimation Programs Interface prediction model was run to predict the half-life in water and sediment for the test chemical. If released in to the environment, 3.77% of the chemical will partition into water according to the Mackay fugacity model level III and the half-life period of test chemical in water is estimated to be 60 days (1440 hrs). The half-life (60 days estimated by EPI suite) indicates that the chemical is persistent in water and the exposure risk to aquatic animals is moderate to high whereas the half-life period of test chemical in sediment is estimated to be 541.66 days (13000 hrs). Based on this half-life value, it indicates that test chemical is persistent in sediment.
Biodegradation in soil
According to this study the test substance Denatonium benzoate is not considered to be a significant degraded substance in 30 days at 15ºC.
Additional information
Biodegradation:
Biodegradation in water:
Experimental study was done by UERL laboratory (2015) in this study a 28-days Manometric respirometry test following the OECD guideline 301F was done to determine the ready biodegradability of the test item Denatonium benzoate (CAS No. 3734-33-6) . The test system included control, test item and reference item. The concentration of test and reference item (Sodium Benzoate) chosen for both the study was 100mg/L, while that of inoculum was 10ml/l. ThOD (Theoretical oxygen demand) of test and reference item was determined by calculation. % Degradation was calculated using the values of BOD and ThOD for test item and reference item. The BOD28value ofDenatonium benzoate (CAS No. 3734-33-6) was observed to be 0.436 mgO2/mg. ThOD was calculatedas 2.40mgO2/mg. Accordingly, the % degradation of the test item after 28 days of incubation at 20 ± 1°C according to manometric respirometry test was obtained to be 18.17%. Based on the results, the test item, under the test conditions, was considered to be non-biodegradable at 20 ± 1°C over a period of 28 days.
Biodegradation in water and sediment
Estimation Programs Interface prediction model was run to predict the half-life in water and sediment for the test chemical. If released in to the environment, 3.77% of the chemical will partition into water according to the Mackay fugacity model level III and the half-life period of test chemical in water is estimated to be 60 days (1440 hrs). The half-life (60 days estimated by EPI suite) indicates that the chemical is persistent in water and the exposure risk to aquatic animals is moderate to high whereas the half-life period of test chemical in sediment is estimated to be 541.66 days (13000 hrs). Based on this half-life value, it indicates that test chemical is persistent in sediment.
Biodegradation in soil
In study of biodegradation of soil of Denatonium benzoate (BITREX) was observed in 4 types of soil namely 1.Kitleyknowe,2. Easter Howgate, 3. Boghall, 4. Balmanno for 30days.Recovery of radioactivity in three of the four soils was more than 80% of the applied radioactivity and was recovered in the top 31.5cm of soil However in the Balmanno soil recovery was much lower ca 60%.
Therefore it is considered that in the study conducted for 30 days at 15ºC no significant degradation of the active substance occurred.
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