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EC number: 248-792-9 | CAS number: 28043-10-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: dermal
Administrative data
- Endpoint:
- acute toxicity: dermal
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1981
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: see 'Remark'
- Remarks:
- GLP study following a method equivalent to a recognised guideline at a limit dose. Minor deviations (abraded skin plus occlusive dressing) to recognised OECD guideline that do not impact the reliability of the study. Since by expert assessment would increase the potential for positive response (evidence of toxicity) by increasing dermal permeability relative to intact skin within the OECD guideline.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 981
- Report date:
- 1981
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 402 (Acute Dermal Toxicity)
- Deviations:
- yes
- Remarks:
- Limit test of 2000 mg/kg bw on clipped-abraded skin.
- Qualifier:
- according to guideline
- Guideline:
- other: 16 CFR 1500.40
- Deviations:
- no
- Principles of method if other than guideline:
- The principles of the method were in accordance with the US 16 CFR 1500.40 method and utilised US 16 CFR 1500.3 definitions.
- GLP compliance:
- yes
- Test type:
- standard acute method
- Limit test:
- yes
Test material
- Reference substance name:
- Methyl 2,6,6-trimethylcyclohex-2-ene-1-carboxylate
- EC Number:
- 248-792-9
- EC Name:
- Methyl 2,6,6-trimethylcyclohex-2-ene-1-carboxylate
- Cas Number:
- 28043-10-9
- Molecular formula:
- C11H18O2
- IUPAC Name:
- methyl 2,6,6-trimethylcyclohex-2-ene-1-carboxylate
- Test material form:
- other: liquid
- Details on test material:
- - Physical state: liquid
Constituent 1
Test animals
- Species:
- rabbit
- Strain:
- New Zealand White
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Recognised animal supplier
- Age at study initiation: Not reported.
- Weight at study initiation: Between 2.0 and 3.0 kg
- Housing: The animals were housed in compliance with US 9 CFR Part 3.
- Diet: ad libitum
- Water: ad libitum
- Acclimation period: Not reported.
ENVIRONMENTAL CONDITIONS
- Temperature (°C): Not reported.
- Humidity (%): Not reported
- Air changes (per hr): Not reported
- Photoperiod (hrs dark / hrs light): Not reported
Administration / exposure
- Type of coverage:
- occlusive
- Vehicle:
- unchanged (no vehicle)
- Details on dermal exposure:
- TEST SITE
- Area of exposure: All rabbits were weighed and the correct amount of experimental material was applied to the back of each animal
- Type of wrap if used: covered with large gauze patches and an impervious material was wrapped snugly around the trunk of each animal. The dressings were removed after twenty-four hours and any excess material was removed and the approximate amount remaining was noted.
REMOVAL OF TEST SUBSTANCE
- Washing (if done): the exposure site was wiped, but not washed, to remove excess material as per US 16 CFR 1500.40.
- Time after start of exposure: 24h
TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 2000 mg/kg - Duration of exposure:
- 24h
- Doses:
- 2000 mg/kg
- No. of animals per sex per dose:
- 3 males and 3 females
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: Observed for signs of toxicity and for mortalities. Gross autopsies were performed on all animals which died during the 14 day observation period and also on all survivors of the 14 day observation period.
- Necropsy of survivors performed: Yes.
Results and discussion
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- > 2 000 mg/kg bw
- Based on:
- test mat.
- Mortality:
- No mortalities.
- Clinical signs:
- other: There were no unusual behavioral signs noted.
- Gross pathology:
- Gross pathologic examination revealed nothing remarkable.
- Other findings:
- No other observations (such as local responses) were noted within the report.
Applicant's summary and conclusion
- Interpretation of results:
- not classified
- Remarks:
- Migrated information Criteria used for interpretation of results: EU
- Conclusions:
- Under the conditions of this study the LD50 was determined to be > 2000 mg/kg via the dermal route in male/female rabbits.
- Executive summary:
The GLP study was performed following a method similar to OECD 402 to assess the dermal toxicity of the test material to the albino rabbit. The test substance was evaluated in six rabbits. A dose of 2000 mg/kg test substance (undiluted), was applied to the back clipped-abraded skin site of the rabbit under a occlusive dressing for 24 hours. After twenty-four hours and any excess material was removed and the approximate amount remaining was noted. The animals were observed for a 14 day period for signs of toxicity and for mortalities. Gross autopsies were performed on all animals. There were no unusual behavioural signs noted and gross pathologic examination revealed nothing remarkable. Under the conditions of this study the LD50 was determined to be greater than 2000 mg/kg via the dermal route in male/female albino rabbits.
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