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EC number: 202-905-8 | CAS number: 100-97-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- publication
- Title:
- Biodegradation of formaldehyde and its derivated in industial watewater with methylotrophic yeast Hansenula polymorpha and with the yeast-bioaugmented activated sludge
- Author:
- Kaszycki, P.; Koloczek, H.
- Year:
- 2 002
- Bibliographic source:
- Biodegradation 13 : 91-99, 2002
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 301 A (Ready Biodegradability: DOC Die Away Test)
- GLP compliance:
- no
Test material
- Reference substance name:
- Methenamine
- EC Number:
- 202-905-8
- EC Name:
- Methenamine
- Cas Number:
- 100-97-0
- Molecular formula:
- C6H12N4
- IUPAC Name:
- 1,3,5,7-tetraazatricyclo[3.3.1.1³,⁷]decane
- Details on test material:
- 1) Methenamine waste water from industrial plan (pH 7.8, COD = 11309 mg/L, formaldehyd + methenamine c = 2300 mg/L, monomeric formaldehyde C= 582 mg/L, methanol C= 2980 mg/L)
2) Pure methenamine solution (1600 mg/L)
Constituent 1
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
1) pH 7.8
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, adapted
- Details on inoculum:
- The study included several independent activated sludge samples collected at aeration chambers of biological wastewater treatment stations of different chemical plant. As an additional reference in yeast integration experiments, the activated sludge from a typical minicipal household wastewater treatment station was also used. Theactivated sludge was applied in experiments within two weeks of collection. The sludge was stored at 4°C and upon experiment, it was reactivated by placing 50-100 ml suspension in a 250 ml flask ans by aerobically cultivationg in a rotary shaker for 48 h at room temperature. Integration of the activated sludge with H. polymorpha was performed by adding the appropriate number of yeast cells to 20-50 mL of the sludge suspension and by cultivating the mixture under aerobic conditions for 24 h at 25°C.
- Duration of test (contact time):
- ca. 48 h
Initial test substance concentration
- Initial conc.:
- ca. 2 300 mg/L
- Based on:
- other: bound and anbound formaldehyd (mainly methenamine) in wastewater experiments
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- test mat. analysis
- Details on study design:
- 1) Measurement of methenamin in wastewater
2) Measurement of pure methenamine
Reference substance
- Reference substance:
- other: no data
Results and discussion
- Preliminary study:
- no data
- Test performance:
- A good test performance was documented.
% Degradationopen allclose all
- Parameter:
- % degradation (test mat. analysis)
- Value:
- ca. 9
- Sampling time:
- 48 h
- Remarks on result:
- other: Wastewater, pH 8.1
- Parameter:
- % degradation (test mat. analysis)
- Value:
- ca. 74
- Sampling time:
- 48 h
- Remarks on result:
- other: Wastewater, pH 4.5
- Details on results:
- 1) Wastewater:
Very poor degradation with H. polymorpha at high ph values of 8.1. At lower pH (4.5) a degradation of 74% was observed.
2) Pure methenamine:
In a model system at pH 5.3, H. polymorpha dens culture (at about 10(exp 7) cells/mL) was able to biodegrade methenamineat initial concentrations up to 1600 mg/L to about 44%.
BOD5 / COD results
- Results with reference substance:
- no reference substance
Applicant's summary and conclusion
- Validity criteria fulfilled:
- not specified
- Interpretation of results:
- readily biodegradable
- Conclusions:
- Methenamine is biodegradable in wastewater at low pH conditions.
- Executive summary:
Methenamine biodegrandation was measured in wastewater containing high amounts of metheamine, formaldehyde and methanol and also in a model system using pure methenamine. The measurement was done at different pH values, by determining the methenamin concentrations colorimentrically after 48 h incubation.
Results:
- Wastewater: Very poor degradation with H. polymorpha at high ph values of 8.1. At lower pH (4.5) a degradation of 74% was observed.
- Pure methenamine: In a model system at pH 5.3, H. polymorpha dens culture (at about 10(exp 7) cells/mL) was able to biodegrade methenamineat initial concentrations up to 1600 mg/L to about 44%.
Thus, methenamine can be regarded biodegradable in wastewater at low pH conditions.
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