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EC number: 915-623-1 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From 14 - 16 May 1991
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP Guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- Principles of method if other than guideline:
- As 2-amylanthraquinone is highly unstable in water under influence of light, the study was conducted under reduced light conditions (<100 lux).
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- - Nominal concentrations: 13, 25, 50, 100 and 200 µg/l.
- Sampling method: At test initiation bulk solutions were sampled and after 24 hours one test vessel per concentration. At 48 hours, all vessels were sampled. - Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: An amount of 20 mg 2-amylanthraquinone was dissolved in 10 ml DMSO resulting in a concentration of 2000 mg/l. This stock solution was diluted with DMSO to obtain stock solutions of 130, 250, 500 and 1000 mg/l. A volume of 60 µl of these solutions was added to 600 ml ISO+ water.
- Controls: A control with DMSO in test medium and a control with test medium were included.
- Chemical name of vehicle: DMSO.
- Concentration of vehicle in test medium: 0.1 ml/l.
- Evidence of undissolved material: No. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Source: TNO, the Netherlands.
- Age at study initiation: 0 - 24 hours
- Method of breeding: Waterfleas are cultured in vessels with 2 liter spring water. They are fed with algal suspension of Chlorella pyrenoidosa and a yeast/Mikromin suspension.
- Feeding during test: not applicable - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Post exposure observation period:
- none
- Hardness:
- 250 mg/l calcium carbonate.
- Test temperature:
- 21.9 - 23.5°C.
- pH:
- Controls: 7.9 - 8.0.
Test concentrations: 7.9 - 8.1 - Dissolved oxygen:
- Controls: 9.0 - 9.1.
Test concentrations: 8.3 - 9.0. - Nominal and measured concentrations:
- See Table 1.
- Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Type: open.
- Material, size, headspace, fill volume: glass 250 ml glass beakers were filled with 200 ml
- Aeration: No.
- Renewal rate of test solution: No.
- No. of organisms per vessel: 10 water fleas.
- No. of vessels per concentration: 3 replicates.
- No. of vessels per control: 3 replicates.
- No. of vessels per vehicle control: 3 replicates.
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: ISO+ water prepared from distilled water.
- Culture medium different from test medium: yes. Water fleas were cultured in spring water.
OTHER TEST CONDITIONS
- Adjustment of pH: No.
- Photoperiod: 16 hours light/8 hours dark
- Light intensity: <100 lux
EFFECT PARAMETERS MEASURED: After 24 and 48 hours the water fleas were observed for immobility, floating on the surface, slower movement, uncontrolled movement, laying down on bottom of test vessel and abnormal shape. - Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 154 µg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- See Table 2.
- Validity criteria fulfilled:
- yes
- Conclusions:
- Short term toxicity of 2-amylanthraquinone to aquatic invertebrates was studied on Daphnia magna in laboratory conditions. Organisms were exposed to nominal concentrations of the test substance: 13, 25, 50, 100 and 200 µg/l. The highest nominal concentration of 200 µg/l was equivalent to 154 µg/l of measured concentration. Observations were carried for 48 hours. EC50 was calculated to be above 154 µg/l.
Reference
Table 2: Immobility of Daphnia magna during the acute toxicity test
Nominal test concentration (µg/l) |
Number of immobile fleas |
Number of water fleas tested |
Percentage of immobility after 48 hours |
|
24 h |
48 h |
|||
0 (control) |
0 |
0 |
30 |
0 |
0 (DMSO-control) |
0 |
0 |
31 |
0 |
13 |
0 |
1 |
31 |
3 |
25 |
0 |
0 |
28 |
0 |
50 |
1 |
1 |
30 |
7 |
100 |
0 |
1 |
30 |
3 |
200 |
0 |
0 |
31 |
0 |
Description of key information
In an OECD 202 test guideline study, Daphnia magna were exposed for 48 hours, static, to 2 -amylanthraquinone. The substance is unstable in water under influence of light, therefore the study was performed under reduced light. Organisms were exposed to nominal concentrations of the test substance: 13, 25, 50, 100 and 200 μg/L. The highest measured concentration was 154 µg/L (comparable to water solubility). The EC50 was calculated to be greater than 154 μg/L (measured concentration).
Key value for chemical safety assessment
Additional information
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