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EC number: 215-311-9 | CAS number: 1321-12-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1976
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Basic data given (No OECD guideline or GLP defined; no necropsy and no histopathological examinations were performed)
Cross-referenceopen allclose all
- Reason / purpose for cross-reference:
- reference to same study
Reference
- Endpoint:
- acute toxicity: dermal
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1976
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Acceptable, well documented study report which meets basic scientific principles
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to same study
- Principles of method if other than guideline:
- Method: other: 5 rats/sex/dose group, 1 dose only as 30 % emulsion covered by aluminium foil fixed by broad stripes of adhesive plaster to back and belly for a 24 hour-exposure period: cleaning with soap and water, observation period: 1 week
- GLP compliance:
- no
- Remarks:
- GLP was not mandatory at the time of the study
- Test type:
- other: Acute dermal toxicity study in rats
- Limit test:
- no
- Specific details on test material used for the study:
- - Name of test material (as cited in study report): 4-Nitrotoluene
- Physical state: solid (yellow crystal)
- Analytical purity: no data - Species:
- rat
- Strain:
- Wistar
- Sex:
- male/female
- Type of coverage:
- occlusive
- Vehicle:
- polyethylene glycol
- Remarks:
- polyethylene glycol 400
- Duration of exposure:
- 24 h
- Doses:
- 750 mg/kg bw in polyethylene glycol 400
- No. of animals per sex per dose:
- 10
- Control animals:
- not specified
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- > 750 mg/kg bw
- Remarks on result:
- other: No mortality occurred.
- Mortality:
- No mortalities could be observed.
- Clinical signs:
- other: After 18 hours after application of the test substance a decrease of general behavior could be observed in the animals. This condition persisted 5 days.
- Interpretation of results:
- Category 3 based on GHS criteria
- Conclusions:
- LD50 (dermal, rat, occlusive, 24h) >750 mg/kg bw.
- Executive summary:
When applied as an emulsion in polyethylene glycol 400 at a dose level of 750 mg/kg bw to the back of 5 rats/sex/group, no deaths during the 24 hour treatment period and during the one week observation period were noted, but the rats showed poor general condition from 18 hours post application up to 4 days after application.
- Reason / purpose for cross-reference:
- reference to same study
Reference
- Endpoint:
- acute toxicity: inhalation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1976
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Basic data given
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to same study
- Principles of method if other than guideline:
- see "Any other information on material and method incl. tables"
- GLP compliance:
- no
- Remarks:
- GLP was not mandatory at the time of the study
- Test type:
- other: acute toxicity inhalation study, rats
- Limit test:
- no
- Specific details on test material used for the study:
- - Name of test material (as cited in study report): 4-Nitrotoluene
- Physical state: solid (yellow crystalline substance)
- Analytical purity: no data - Species:
- rat
- Strain:
- Wistar
- Sex:
- male
- Route of administration:
- inhalation: dust
- Type of inhalation exposure:
- whole body
- Vehicle:
- other: air
- Analytical verification of test atmosphere concentrations:
- not specified
- Duration of exposure:
- 1 h
- Concentrations:
- 4167 mg/m³ air.
- No. of animals per sex per dose:
- 5
- Control animals:
- not specified
- Sex:
- male
- Dose descriptor:
- LC50
- Effect level:
- > 4 167 mg/m³ air
- Exp. duration:
- 1 h
- Remarks on result:
- other: No mortality occurred.
- Mortality:
- No martality occurred.
- Clinical signs:
- other: No signs of intoxication during the one hour exposure time or the 7 days post exposure observation period could be observed.
- Interpretation of results:
- Category 4 based on GHS criteria
- Conclusions:
- LC50 (rat, inhaltion, 1h) > 4167 mg/m³ air.
- Executive summary:
In an acute inhalation toxicity study male Wistar rats were exposed to the test-substance in a dose of 4167 mg/m³ air.
No signs of intoxication during the one hour exposure time or the 7 days post exposure observation period could be observed.
Therefore the LC 50 was > 4167 mg/m³ air/1h.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 976
- Report date:
- 1976
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- Deviations:
- yes
- Remarks:
- No GLP study. Analytical purity not reported. Animals were not fasted before the treatment. Enviromental conditions not reported. Acclimation period not reported. Body weights not reported. No necropsy and no histopathological examinations were performed
- GLP compliance:
- no
- Remarks:
- GLP was not mandatory at the time of the study
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- 4-nitrotoluene
- EC Number:
- 202-808-0
- EC Name:
- 4-nitrotoluene
- Cas Number:
- 99-99-0
- Molecular formula:
- C7H7NO2
- IUPAC Name:
- 1-methyl-4-nitrobenzene
- Test material form:
- solid
Constituent 1
- Specific details on test material used for the study:
- - Name of test material (as cited in study report): 4-nitrotoluene
- Physical state: solid
- Analytical purity: not reported
Test animals
- Species:
- rat
- Strain:
- other: Wistar-II-R
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Weight at study initiation: 160-245 g
- Housing: animals were housed in Makrolon cages, type 3
- Diet (e.g. ad libitum): Altromin-Standarddiät (Altromin GmbH, Lage/Lippe, Germany) ad libitum
- Water (e.g. ad libitum): ad libitum
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- polyethylene glycol
- Remarks:
- Polyethylenglycol 400
- Doses:
- 100, 250, 500, 1000, 2250 mg/kg bw
- No. of animals per sex per dose:
- 15
- Control animals:
- other: not applicable
- Details on study design:
- - Duration of observation period following administration: 14 days
- Other examinations performed: clinical signs
Results and discussion
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- > 2 250 mg/kg bw
- Remarks on result:
- other: No mortality occured
- Mortality:
- Deaths did not occur at the doses specified.
See "Remarks on results tables and figures". - Clinical signs:
- other: Symptoms of poisoning began in rat from 4 to 40 minutes after application in the form of disordered breathing and a reduced general condition. The respiratory disturbances were observed until 3 days after application, and the general condition was to be r
Any other information on results incl. tables
|
|
Symptoms of poisoning
|
Appereance ofdeath |
|
Dosis mg/kg |
Toxicological results |
Start |
End |
|
Male rats |
||||
100 |
0/0/15 |
- |
- |
- |
250 |
0/15/15 |
29´ |
2d |
- |
500 |
0/15/15 |
21´ |
4d |
- |
1000 |
0/15/15 |
18´ |
4d |
- |
2250 |
0/15/15 |
4´ |
6d |
- |
Female rats |
||||
100 |
0/0/15 |
- |
- |
- |
250 |
0/15/15 |
40´ |
3d |
- |
500 |
0/15/15 |
35´ |
4d |
- |
1000 |
0/15/15 |
20´ |
5d |
- |
2250 |
0/15/15 |
12´ |
5d |
- |
In this table in the column "Toxicological results" the numbers have the following meaning:
1stnumber= amount of dead animals
2ndnumber = amount of animals with symptoms
3rdnumber = amount of animals used
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- LD50 (oral, rat) > 2250 mg/kg bw.
- Executive summary:
In an acute oral toxicity study male rats received the test substance in a dose of 100, 250, 500, 1000 or 2250 mg/kg bw in Polyethylenglycol 400. Symptoms of poisoning began in rat from 4 to 40 minutes after application in the form of disordered breathing and a reduced general condition. The respiratory disturbances were observed until 3 days after application, and the general condition was to be reduced to 6 days. No mortalities were observed. Therefore the LD 50 was >2250 mg/kg bw.
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