Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 200-539-3 | CAS number: 62-53-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to terrestrial plants
Administrative data
Link to relevant study record(s)
Description of key information
NOEC(14d): 0.3 mg/m3 Brassica pekinensis
EC50(14d): 33 mg/kg soil d.w. Lactuva sativa
Key value for chemical safety assessment
Additional information
Aniline reaches soils via deposition from the atmosphere or by degradation of plant protection agents. As already stated in section 5.2.3 aniline can be biodegraded or be bound onto the soil organics, where the reaction product accumulates. Therefore, for the assessment of plant toxicity of Aniline, one has to consider both, the soil and the atmospheric compartment. Reliable short term toxicity tests are available for both compartments.
Atmospheric compartment:
To allow the derivation of a PNECpIant for a risk assessment of the atmosphere, a plant fumigation test was performed with aniline (BASF, 2002). Three species of higher plants (Avena sativa, Brassica pekinensis and Abies grandis) were exposed in laboratory exposure chambers for 14 days to 3 aniline concentrations (0.1 mg/m3, 0.3 mg/m3 and 1 mg/m) and a control. Aniline concentrations were measured by HPLC. Daily means were calculated based on two measured samples per concentration and exposure. Mean measured concentrations were 0.158 mg/m3, 0.334 mg/m3 and 1.22 mg/m3. Seeds (Avena and Brassica) and germinated plants (Abies, 1 year old plants) were acclimatised in the exposure chambers for 8 days before exposure to aniline. Endpoints were plant length, wet and dry weight as well as macroscopic changes for Avena sativa and Brassica pekinensis. For Abies grandis only macroscopic and microscopic changes were recorded. The EC50 for all tested parameters for Avena sativa and Brassica rapa was > 1 mg/m3. The NOEC values for all tested endpoints were 1 mg/m3 for Avena sativa and 0.3 mg/m3 for Brassica pekinensis. Macroscopic and stereo microscopic observations in Abies grandis showed no changes in the habit of the plants. For the derivation of the PNECplant the lowest NOEC of 0.3 mg/m3 found for Brassica pekinensis is used as basic value. An assessment factor of 50 is proposed as the exposure period for the three tested plant species was only 14 days. There are indications from a study of Cheeseman et al. (1980) that certain tree species may react sensitive to aniline. As it is not appropriate to examine adequately the effect of aniline on trees in a 14-day laboratory test this is considered by the height of the assessment factor.
Therefore PNECplants = 0.3 mg/m3 / 50 = 6 μg/m3
Soil compartment:
Hulzebos et al. (1993) determined the toxicity of aniline to Lactuca sativa in natural soil and in nutrient solution. Both tests were conducted by two institutes (TNO and RIVM). A 14-day EC50 for growth inhibition of 33 mg/kg soil (dw) was found by RIVM and of 56 mg/kg soil (dw) by TNO. The content of organic matter was 1.4% for the soil used by TNO and 1.8% for the soil used by RIVM. For the test with nutrient solution different exposure times were used by the two laboratories. TNO derived a 14-day EC50 of 7.9 mg/l and RIVM found for 21-day exposure an ECSO of 17 mg/l. All effect values are related to nominal concentrations. The authors showed that the concentration of aniline had dropped to < 30% after 14 days.
The lowest EC50 value found for soil exposure is used for the determination of the PNEC50. An assessment factor of 1,000 has to be applied. This leads to a PNEC of 33 μg/kg (dry weight).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.