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EC number: 232-094-6 | CAS number: 7786-30-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- October 2009 till March 2010
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 010
- Report date:
- 2010
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 405 (Acute Eye Irritation / Corrosion)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.5 (Acute Toxicity: Eye Irritation / Corrosion)
- Version / remarks:
- May 2008
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 870.2400 (Acute Eye Irritation)
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- magnesium chloride hexahydrate
- IUPAC Name:
- magnesium chloride hexahydrate
- Reference substance name:
- 7791-18-6
- Cas Number:
- 7791-18-6
- IUPAC Name:
- 7791-18-6
- Details on test material:
- water content (specification): 51-55% (53.4%)
Colour: colourless
Physical state: solid, crystals
Storage: at room temperature, in a tightly closed package
Solvent: water
Stability after opening: instable after repeated contact to air
pH: 5.5 - 7.0 (5% solution at 20° C)
Constituent 1
Constituent 2
- Specific details on test material used for the study:
- - Name of test material (as cited in study report): Magnesium chloride hexahydrate
- Physical state: Colourless; Solid, crystals
- Stability after opening: Instable after repeated contact to air
- Storage condition of test material: At room temperature, in a tightly closed package.
- pH: 5.5 - 7.0 (5% solution at 20 °C)
- Solvent: Water
No further information on the test material was stated.
Test animals / tissue source
- Species:
- rabbit
- Strain:
- New Zealand White
- Details on test animals or tissues and environmental conditions:
- Species/strain: Healthy New Zealand White Rabbits, Crl: KBL (NZW)
Source: Charles River Deutschland, D-97633 Sulzfeld
Sex: female
Body weight at the beginning of the study: > 2kg
Age at the beginning of the study: approximately 21 weeks old
The animals were derived from a controlled full barrier maintained breeding system (SPF).
Housing and Feeding Conditions: semi-barrier in an air-conditioned room, temperature: 18 (+/-3)°C, relative humidity: 55 (+/-10)%, artificial light, sequence being 12 hours light, 12 hours dark, air change: at least 10 x / hour, free access to autoclaved hay and to Altromin 2123 maintenance diet for rabbits, rich in crude fibre, free access to water, housed in ABS - plastic rabbit cages, floor 4200 cm2 and adequate acclimatisation period (at least 5 days).
Test system
- Vehicle:
- unchanged (no vehicle)
- Controls:
- other: The untreated contralateral eye served as control.
- Amount / concentration applied:
- A dose of 0.1 g of the test item was applied to the test site.
- Duration of treatment / exposure:
- 72 hours
- Observation period (in vivo):
- Observation at 1, 24, 48, and 72 hours and 4 to 6 days
- Number of animals or in vitro replicates:
3 female rabbits- Details on study design:
- Approximately 24 hours before the test and immediately prior to the application both eyes of each animal were examined. A health inspection was performed to ensure the good state of health of the animals. None of the animals showed eye irritation, ocular defects, or pre-existing corneal injury.
The test item was applied at a single dose in the conjunctival sac of one eye of each test animal after pulling the lower lid away from the eyeball. The lids were then gently held together for about 1 second in order to prevent loss of the material. 72 hours post-instillation as well as at the end of the prolonged observation period the treated eyes were examined with the aid of a fluorescein solution
The eyes were examined for signs of irritation throughout the observation period. Individual reactions of each animal were recorded at each time of observation. The observations were recorded by a toxicologist and confirmed by another. Nature, severity and duration of all lesions observed were described. For the calculation only the 24-, 48- and 72-hour readings were used.
Results and discussion
In vivo
Resultsopen allclose all
- Irritation parameter:
- cornea opacity score
- Basis:
- mean
- Time point:
- 24/48/72 h
- Score:
- ca. 0
- Max. score:
- 0
- Remarks on result:
- other: Each animal showed no cornea opacity at any time
- Irritation parameter:
- iris score
- Basis:
- mean
- Time point:
- 24/48/72 h
- Score:
- ca. 0
- Max. score:
- 0
- Remarks on result:
- other: Each animal showed no iris effect at any time
- Irritation parameter:
- conjunctivae score
- Basis:
- animal #1
- Time point:
- 24/48/72 h
- Score:
- ca. 1.67
- Max. score:
- 2
- Reversibility:
- fully reversible within: 6 days
- Irritation parameter:
- chemosis score
- Basis:
- animal #1
- Time point:
- 24/48/72 h
- Score:
- ca. 0.67
- Max. score:
- 3
- Reversibility:
- fully reversible within: 72 hours
- Irritation parameter:
- conjunctivae score
- Basis:
- animal #2
- Time point:
- 24/48/72 h
- Score:
- ca. 1.33
- Max. score:
- 2
- Reversibility:
- fully reversible within: 4 days
- Irritation parameter:
- chemosis score
- Basis:
- animal #2
- Time point:
- 24/48/72 h
- Score:
- ca. 1
- Max. score:
- 2
- Reversibility:
- fully reversible within: 4 days
- Irritation parameter:
- conjunctivae score
- Basis:
- animal #3
- Time point:
- 24/48/72 h
- Score:
- ca. 0.67
- Max. score:
- 2
- Reversibility:
- fully reversible within: 48 hours
- Irritation parameter:
- chemosis score
- Basis:
- animal #3
- Time point:
- 24/48/72 h
- Score:
- ca. 0.33
- Max. score:
- 2
- Reversibility:
- fully reversible within: 48 hours
- Other effects:
- There were no significant body weight changes, no individual systemic and local findings during the observation period.
Any other information on results incl. tables
Eye Irritation Scores – Animal No. 1
|
Single data |
Average score (24, 48 and 72 hours) |
|||
Time post application |
|||||
1 hour |
24 hours |
48 hours |
72 hours |
|
|
T/C |
T/C |
T/C |
T/C |
|
|
Cornea |
0/0 |
0/0 |
0/0 |
0/0 |
0 |
Iris |
0/0 |
0/0 |
0/0 |
0/0 |
0 |
Conjunctival redness |
2/0 |
2/0 |
2/0 |
1/0 |
1.67 |
Conjunctival chemosis |
3/0 |
1/0 |
1/0 |
0/0 |
0.67 |
|
Single data |
||||
Time post application |
|||||
4 days |
5 days |
6 days |
7 days |
8 days |
|
T/C |
T/C |
T/C |
T/C |
T/C |
|
Cornea |
0/0 |
0/0 |
0/0 |
- |
- |
Iris |
0/0 |
0/0 |
0/0 |
- |
- |
Conjunctival redness |
1/0 |
1/0 |
0/0 |
- |
- |
Conjunctival chemosis |
0/0 |
0/0 |
0/0 |
- |
- |
Eye Irritation Scores – Animal No. 2
|
Single data |
Average score (24, 48 and 72 hours) |
|||
Time post application |
|||||
1 hour |
24 hours |
48 hours |
72 hours |
|
|
T/C |
T/C |
T/C |
T/C |
|
|
Cornea |
0/0 |
0/0 |
0/0 |
0/0 |
0 |
Iris |
0/0 |
0/0 |
0/0 |
0/0 |
0 |
Conjunctival redness |
2/0 |
2/0 |
1/0 |
1/0 |
1.33 |
Conjunctival chemosis |
2/0 |
1/0 |
1/0 |
1/0 |
1.0 |
|
Single data |
||||
Time post application |
|||||
4 days |
5 days |
6 days |
7 days |
8 days |
|
T/C |
T/C |
T/C |
T/C |
T/C |
|
Cornea |
0/0 |
- |
- |
- |
- |
Iris |
0/0 |
- |
- |
- |
- |
Conjunctival redness |
0/0 |
- |
- |
- |
- |
Conjunctival chemosis |
0/0 |
- |
- |
- |
- |
Eye Irritation Scores – Animal No.3
|
Single data |
Average score (24, 48 and 72 hours) |
|||
Time post application |
|||||
1 hour |
24 hours |
48 hours |
72 hours |
|
|
T/C |
T/C |
T/C |
T/C |
|
|
Cornea |
0/0 |
0/0 |
0/0 |
0/0 |
0 |
Iris |
0/0 |
0/0 |
0/0 |
0/0 |
0 |
Conjunctival redness |
2/0 |
2/0 |
0/0 |
0/0 |
0.67 |
Conjunctival chemosis |
2/0 |
1/0 |
0/0 |
0/0 |
0.33 |
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- Under the conditions of this study, MgCl2, 6H2O is not irritating to eye.
- Executive summary:
Under the conditions of this study, single ocular instillation of the test item Magnesium chloride hexahydrate to rabbits at a dose of 0.1 g produced irritant effects, which were fully reversible within 6, 4 and 2 days post-instillation in animal no. 1, 2 and 3, respectively.
Neither mortalities nor significant clinical signs of toxicity were observed.
According to the GHS criteria, the MgCl2, 6H20 is not irritating to eyes.
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