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EC number: 210-478-4 | CAS number: 616-38-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2010
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Deviations:
- yes
- Remarks:
- The temperature was lower than stated in the guideline. As no mortality occurred during the test, this deviation was stated as not critical
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- yes
- Remarks:
- The temperature was lower than stated in the guideline. As no mortality occurred during the test, this deviation was stated as not critical
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 0 and 100 mg/L
- Sampling method: 1.5 mL sample was required for measurement. Each sample was collected from the middle of the aquarium. Care was taken not to collect solid particles (excrements). The unfiltered samples were directly injected in the gas chromatograph
- Sample storage conditions before analysis: The samples were stored in a fridge at 2 – 8°C until measurement.
samples were withdrawn from the treatment solutions at 0, 24, 48, 72 and 96 hours. Concentrations of the test article were determined by a GC chromaotographic method of analysis. - Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: flow-through
- Differential loading
- Controls: dechlorinated tap water
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): water
- Concentration of vehicle in test medium (stock solution and final test solution(s) including control(s)): controls: 0 mg/L; stock solution: 5g/L; final test solutions: 100 mg/L
- Evidence of undissolved material (e.g. precipitate, surface film, etc): none stated - Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: not stated
- Strain: HAMILTON-BUCHANAN
- Source: Dehner Garten Centre, D-67433 Neustadt
- Age at study initiation (mean and range, SD): not stated. Fish were bought as sexually immature young fish, length 2 +/- 1 cm. They were kept for 14 days under test conditions before being used in the test
- Length at study initiation (length definition, mean, range and SD): not stated
- Weight at study initiation (mean and range, SD): not stated
- Method of breeding: not stated
- Feeding during test: three times a day with warm water fishfood and daphnia, totalling to about 1-2% of body weight per day
- Food type: warm water fishfood
- Amount: 1 - 2% of body weight per day
ACCLIMATION
- Acclimation period: 14 days
- Acclimation conditions (same as test or not): same as test
- Type and amount of food: warm water fishfood, 1 - 2% of body weight per day
- Health during acclimation (any mortality observed): mortality in this period was not more than 5% - Test type:
- flow-through
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Post exposure observation period:
- Not stated
- Hardness:
- 1.18 mmol/L (analysis of dilution water 30.10.2010)
- Test temperature:
- 23+/- 1 oC
- pH:
- 8.0 - 8.3
- Dissolved oxygen:
- Above 82% throughout the test
- Salinity:
- not stated
- Nominal and measured concentrations:
- See tabulated results below
- Details on test conditions:
- TEST SYSTEM
- Test vessel: polyethylene aquaria
- Type (delete if not applicable): open / closed: not stated
- Material, size, headspace, fill volume: not stated
- Aeration: not stated
- Type of flow-through (e.g. peristaltic or proportional diluter): not stated
- Renewal rate of test solution (frequency/flow rate): medium renewal was twice a week
- No. of organisms per vessel: 7
- No. of vessels per control: 1
- No. of vessels per vehicle control: not stated
From the study text there was one vessel containg 10.5L test solution and seven fish and one vessel containing 10.5L dilution water and seven fish.
- Biomass loading rate: not stated
TEST MEDIUM / WATER PARAMETERS
Parameter Value Unit
sodium 3.8 mg/L
potassium 2.4 mg/L
calcium 23.8 mg/L
magnesium 1.9 mg/L
aluminium < 0.01 mg/L
iron < 0.005 mg/L
mangane < 0.001 mg/L
ammonium < 0.05 mg/L
nitrate < 1 mg/L
nitrite < 0.01 mg/L
chloride 7 mg/L
sulphate 8 mg/L
TOC < 1.0 mg/L
antimony < 0.001 mg/L
arsenic < 0.001 mg/L
lead < 0.001 mg/L
cadmium < 0.0002 mg/L
chromium < 0.001 mg/L
copper 0.001 mg/L
nickel < 0.001 mg/L
mercury < 0.0001 mg/L
selenium < 0.001 mg/L
bor < 0.005 mg/L
cyanide < 0.01 mg/L
fluoride < 0.2 mg/L
benzene < 0.25 µg/l
PAH < 0.004 µg/L
COC < 1.0 µg/L
pesticides and biocides < LOD
pH 8.23
conductivity at 20 °C 142 µS/cm
hardness 1.18 mmol/L
LOD = limit of detection
COC: chlorinated organic compounds
OTHER TEST CONDITIONS
- Adjustment of pH: none
- Photoperiod: 12/12 hours
- Light intensity: neon tubes - Reference substance (positive control):
- no
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks:
- No abnormal behaviourof the surviving fish was observed
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- - Behavioural abnormalities:
- Observations on body length and weight: none reported
- Other biological observations: none
- Mortality of control: zero
- Other adverse effects control: none
- Abnormal responses: none
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: none stated
- Effect concentrations exceeding solubility of substance in test medium: no - Reported statistics and error estimates:
- It was not possible to determine the 95% confidence interval for any of the endpoints (NOEC, LC50, LC100)
- Sublethal observations / clinical signs:
Analytical determinations
Measurement
Nominal Concentration mg/L
0
100
Measured Conc. in mg/L 0h Replicate 1
n. d.
124
Measured Conc. in mg/L 0h Replicate 2
n. d.
76
Mean in mg/L
n. d.
100
Measured Conc. in mg/L 24h Replicate 1
n. d.
86
Measured Conc. in mg/L 24h Replicate 2
n. d.
82
Mean in mg/L
n. d.
84
Measured Conc. in mg/L 48h Replicate 1
n. d.
60
Measured Conc. in mg/L 48h Replicate 2
n. d.
57
Mean in mg/L
n. d.
58
Measured Conc. in mg/L 72h Replicate 1
n. d.
115
Measured Conc. in mg/L 72h Replicate 2
n. d.
116
Mean in mg/L
n. d.
116
Measured Conc. in mg/L 96h Replicate 1
n. d.
109
Measured Conc. in mg/L 96h Replicate 2
n. d.
85
Mean in mg/L
n. d.
97
Geometric Mean in mg/L
n. d.
89
n. d. = not detectable
Mortalities
Nom. Conc. in mg/L
Total of fish used
dead fish
24 h
48 h
72 h
96 h
0
7
0
0
0
0
100
7
0
0
0
0
Biological results
Parameter
Value
95%-confidence-interval
24h NOEC
100 mg/L
not determinable
24h LC50
> 100 mg/L
not determinable
24h LC100
> 100 mg/L
not determinable
48h NOEC
100 mg/L
not determinable
48h LC50
> 100 mg/L
not determinable
48h LC100
>100 mg/L
not determinable
72h NOEC
100 mg/L
not determinable
72h LC50
> 100 mg/L
not determinable
72h LC100
> 100 mg/L
not determinable
96h NOEC
100 mg/L
not determinable
96h LC50
> 100 mg/L
not determinable
96h LC100
> 100 mg/L
not determinable
- Validity criteria fulfilled:
- yes
- Conclusions:
- The toxicity against Danio rerio was tested using a flow-through test design.
The main study was performed as a limit test with 100 mg/L nominal concentration. Seven fish were exposed to the test item for 96 hours. There were no mortalities in the treated group.
At the start and at the end of the test and every 24 hours, the content of the test item in the test solutions was determined using gas chromatography.The determination of the results was based on the nominal concentration.
The following results for the test item dimethyl carbonate could be determined:
96h NOEC = 100 mg/L
96h LC50 > 100 mg/L
The validity criteria for this test were fulfilled
Reference
Description of key information
the 96h LC50 for freshwater fish was determined to be >100 mg/L
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 100 mg/L
Additional information
the 96h NOEC for freshwater fish was determined to be 100 mg/L
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