3(or 4)-methylbenzene-1,2-diamine

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Full report, pre guideline and GLP

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1974

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

Single group exposure whole body to a concentration that was rising over the test period of 220 minutes.

GLP compliance:

no

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

3(or 4)-methylbenzene-1,2-diamine

EC Number:

248-145-0

EC Name:

3(or 4)-methylbenzene-1,2-diamine

Cas Number:

26966-75-6

Molecular formula:

C7H10N2

IUPAC Name:

3(or 4)-methylbenzene-1,2-diamine

Test animals

Species:

rat

Strain:

Wistar

Sex:

not specified

Details on test animals or test system and environmental conditions:

Rats weighing between 200 and 300 g were used.
Maintainence was according to SOPs with ad lib food and water except during the exposure period.

Administration / exposure

Route of administration:

inhalation: vapour

Type of inhalation exposure:

whole body

Vehicle:

other: unchanged (no vehicle)

Details on inhalation exposure:

Animals were kept in individual wiremesh cages for exposure. The inhalation chamber was a 26.5 litre stainless steel jar mounted horizontally sealed with a transparent plexiglass sheet providing for air intake, exhaust and sampling.
Test material was held in a glass container in an oil bath kept at 130ºC, and vapours blown from the surface to the chamber at 180 l/hr.

Analytical verification of test atmosphere concentrations:

yes

Remarks:

GC method

Duration of exposure:

4 h

Concentrations:

The concentrations analysed at 60, 120 and 180 minutes were 209, 500 and 1300ppm respectively.
This increase was ascribed to an increase in temperature of the melted material.

No. of animals per sex per dose:

10

Control animals:

yes

Details on study design:

Single group, whole body exposure to a concentration that was rising over the test period of 220 minutes.
Control rats were held in the chamber without test material exposure.
Observation after exposure was for 14 days.
Two rats were sacrifices after 24 hours for examination.
Other animals were sacrificed after 14 days and autopsied.

Statistics:

none

Results and discussion

Effect levels

Sex:

not specified

Dose descriptor:

LCLo

Effect level:

> 670 ppm

Remarks on result:

other: Effect level mean of chamber concentrations during exposure

Mortality:

None

Clinical signs:

other: Increased blinking and grooming during exposure.

Body weight:

no data

Gross pathology:

None seen

Other findings:

none

Any other information on results incl. tables

No adverse effects seen during exposure, during 14 days observation or on post mortem examination.

The LDlow is taken as a mean of the three chamber analysed concentrations. While this is a measured concentration the lack of adverse effects indicate the LClow is likely to be considerably higher than this value.