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EC number: 227-561-6 | CAS number: 5888-33-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2012-07-20 - 2012-09-28
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Remarks:
- OECD 203, GLP, analytical monitoring.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- Analytical evaluation of the concentrations of Isobornyl acrylate and the control were carried out via GC-FID from freshly prepared media after 0 and 72 h, and from 24 h old test media after 24 and 96 h.
- Vehicle:
- no
- Details on test solutions:
- Dilution water: purified drinking water of local origin
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- All fish used in the test were gained at Dr.Noack-Laboratorien, from a single brood stock (supplier: Umweltbundesamt, Schichauweg 58, D-12307 Berlin).
Holding:
Fish were held at the test facility at 23 2 °C and diffuse light (0.1-10 µmol photons • m-2 • s-1, natural photoperiod).
The water was changed at least once per week.
The dissolved oxygen concentration was more than 80 % of the air saturation value.
Zebrafish with at least 12 days of acclimatisation and mortality < 5 % within this period of time were used in the test. No disease treatments were administered throughout holding and testing.
Food (Sera Vipan; Sera GmbH, D-52518 Heinsberg) was provided 3 times per week. The amount of food was 4 % of the fish body weight per feeding day.
The test fish were not fed 24 h before the test started. - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Hardness:
- 52 mg CaCO3/L
- Test temperature:
- 21.7 ± 0.46 °C
- pH:
- varied between 7.0 and 7.5
- Dissolved oxygen:
- in all test vessels between 85 % and 100%
- Nominal and measured concentrations:
- see table below.
- Details on test conditions:
- Dilution water was prepared according to the test guideline (OECD 203) following internal SOP’s.
Test concentrations:
Aliquots of the stock solution were shaken for 24 h, then they were diluted with purified drinking water (dilution water).
Test priciples and procedure:
10 fish were used for each test concentration including controls.
Only fish in good health and free from any apparent malformation were used.
15-l glass aquaria were used as test vessels and filled with 10 l of test solution. The test animals were added to the previously prepared test solution; controls were kept in dilution water (blank).
The aquaria were slightly aerated via a glass capillary throughout the test.
The fish were exposed to various concentrations of the test substance for a period of 96 hours under semi static conditions. The test medium was changed completely every day. Visible abnormalities observed during the first 6 hours of the experiment were recorded. Mortalities and visual abnormalities were recorded at 24 h, 48 h, 72 h, and 96 h.
The test temperature was 21.8° C to 22.1 ° C. The pH of the dilution water was 7.8 to 8.1. The oxygen saturation in all test vessels was between 86 % and 100 %.
The light/dark rhythm was 14h/10h.
The pH, temperature and the oxygen concentrations of the control and of all the test concentrations were measured daily. - Reference substance (positive control):
- not required
- Remarks:
- No reference item is recommended for this test according to the guidelines.
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 0.704 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 95% CL
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- 0.431 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- 1.15 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- see tables OBSERVATIONS and CUMULATIVE MORTALITY (below)
- Reported statistics and error estimates:
- Equation: 0 to 100, sigmoidal dose-response, variable slope Y=100/(1+10^((logEC50-X)*Hill Slope))
- Sublethal observations / clinical signs:
Concentrations of Isobornyl acrylate
Sampling date
2012-09-24
fresh media; 0 h2012-09-25
old media; 24 h2012-09-27
fresh media; 72 h2012-09-28
old media; 96 hStart of analysis
2012-09-24
2012-09-25
2012-09-27
2012-09-28
Isobornyl acrylate
Nominal test item conc.
[mg/L]Meas. conc.
[mg/L]RR
[%]Meas. conc.
[mg/L]RR
[%]Meas. conc.
[mg/L]RR
[%]Meas. conc.
[mg/L]RR
[%]Geometric mean measured conc.
[mg/L]8.0
6.841)
86
5.76
72
*
*
6.28
3.64
3.031)
83
2.28
63
*
*
2.63
1.65
1.201)
72
0.895
54
1.66
101
0.990
60
1.15
0.751
0.3611)
48
0.298
40
0.728
97
0.439
58
0.431
0.341
0.1201)
35
0.101
30
0.312
91
0.169
50
0.159
0.155
0.07371)
48
0.0628
41
0.09172)
59
0.0666
43
0.0732
Control
< LOQM
< LOQM
< LOQM
< LOQM
< LOQM
conc. = concentration
Meas. conc. = Measured concentration of the test item (dilution factor taken into account)
RR = Recovery rate related to the nominal concentration of the test item
LOQM = Limit of quantification of theanalytical method(0.01 mg/L of the test item)
* = no samples due to 100 % Mortality
1) = Reanalysed 25.09.2012, mean value of 3 Injections (dilution factor taken into account).
2) = Reanalysed 28.09.2012, mean value of 3 Injections (dilution factor taken into account).
OBSERVATIONS
Geometric mean test item concentration
Effect *
Number of fish affected at observation time [hours]
[mg/L]
24
48
72
96
6.28
(E)
7/7
---
---
---
2.63
(E)
3/7
4/4
---
---
(2.3)
4/7
-
---
---
1.15
(E)
-
-
5/7
2/2
(2.3)
-
-
2/7
-
(1)
7/7
7/7
-
-
0.431
(1)
7/7
7/7
7/7
7/7
0.159
(1)
7/7
7/7
7/7
7/7
0.073
(1)
7/7
7/7
7/7
7/7
Control
(1)
7/7
7/7
7/7
7/7
*) The numbers in brackets correspond to the following observations:
(1) =behaviour
(2.3) = Staggering (E) = Exitus letalis
- = No observations --- = No observation due to 100 % mortality
CUMULATIVE MORTALITY
Geometric mean
test item concentrationCumulative mortality [%] at observation time [hours]
[mg/L]
24
48
72
96
6.28
100
100
100
100
2.63
43
100
100
100
1.15
0
0
71
100
0.431
0
0
0
0
0.159
0
0
0
0
0.073
0
0
0
0
Control
0
0
0
0
- Validity criteria fulfilled:
- yes
- Conclusions:
- In a valid guideline study (OECD 203, semi-static conditions), the 96 hr LC50 for Danio rerio (Teleostei, Cyprinidae) is 0.704 mg/L .
- Executive summary:
In a valid 96-hr acute toxicity study according to OECD TG 203 with GLP, Danio rerio were exposed to Isobornyl acrylate at 0 (control), 0.155 – 0.341 – 0.751 – 1.65 – 3.64 – 8.00 mg/L nominal and 0 (control), 0.073 – 0.159 – 0.431 – 1.15 – 2.63 – 6.28 mg/L measured concentrations under semi-static conditions.
The 96-h LC50was 0.704 (0.431 – 1.15 95 % confidence limits) mg a.i./L based on mortality.
The LC100and NOEC values, based on mortality effects, were 1.15 mg/L and 0.431 mg a.i./L, respectively. The effect concentrations refer to the geometric mean measured concentrations.
Results Synopsis
LC50: 0.704 mg a.i./L 95% C.I.: 0.431 - 1.15 mg a.i./L
NOEL: 0.431 mg a.i./L
LC100: 1.15 mg a.i./L}
Endpoint(s) Effected: mortality
NOTE: Any of data in this dataset are disseminated by the European Union on a right-to-know basis and this is not a publication in the same sense as a book or an article in a journal. The right of ownership in any part of this information is reserved by the data owner(s). The use of this information for any other, e.g. commercial purpose is strictly reserved to the data owners and those persons or legal entities having paid the respective access fee for the intended purpose.
Reference
Description of key information
One reliable study for Isobornyl acrylate on freshwater fish acc. OECD 203 is available. LC50 (96h) on Danio rerio was determined to be 0.704 mg/L
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Dose descriptor:
- LC50
- Effect concentration:
- 0.704 mg/L
Additional information
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