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EC number: 233-032-0 | CAS number: 10024-97-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to reproduction: other studies
Administrative data
- Endpoint:
- toxicity to reproduction: other studies
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Non-GLP, no TG available
Data source
Reference
- Reference Type:
- publication
- Title:
- Testicular reaction to prolonged exposure to nitrous oxide
- Author:
- Kripke, B.J., Kelman, A.d., Shah, N.K., Balogh, K. & Handler, A.H.
- Year:
- 1 976
- Bibliographic source:
- Anesthesiology, 44(2); pp 104-113
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- Mechanistic study examining the effects of N2O on testicular function
- GLP compliance:
- no
- Type of method:
- in vivo
Test material
- Reference substance name:
- Dinitrogen oxide
- EC Number:
- 233-032-0
- EC Name:
- Dinitrogen oxide
- Cas Number:
- 10024-97-2
- Molecular formula:
- N2O
- IUPAC Name:
- Dinitrogen Oxide
- Test material form:
- gas under pressure: liquefied gas
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Sprague-Dawley
- Sex:
- male
Administration / exposure
- Route of administration:
- inhalation: gas
- Type of inhalation exposure (if applicable):
- whole body
- Vehicle:
- air
- Analytical verification of doses or concentrations:
- yes
- Duration of treatment / exposure:
- Either 8 h/d or 24 h/d
- Frequency of treatment:
- Either 8 h/d or 24 h/d for 1, 2, 3, 4, 5, 7, 10, 14, 21, 28, 32 or 35 d
- Duration of test:
- up to 35 d
Doses / concentrations
- Remarks:
- Doses / Concentrations:
0, 20%
Basis:
nominal conc.
- No. of animals per sex per dose:
- 4-6/gp
Results and discussion
Effect levels
- Dose descriptor:
- NOAEC
- Remarks on result:
- not determinable
- Remarks:
- no NOAEC identified
Observed effects
Following continuous exposure over 35 d resulted in tubules almost empty of spermatids and spermatozoa. Intermittent exposure had milder effects typified by a reduction in the number of spermatogenic cells. Following 6 d without exposure there was clear evidence of recovery of spermatogenesis but in some animals the recovery took longer. There was no effect on testosterone level which was consistent with the lack of any histological changes in interstitial cells.
Applicant's summary and conclusion
- Conclusions:
- In conclusion, continual exposure to N2O resulted in depletion of spermatids and spermatozoa, which was reversible upon cessation of exposure. No histopathological changes were present in interstitial cells, with testosterone levels remaining unaffected.
- Executive summary:
Groups of approximately 45 M rats were exposed to 20%N2 for either 8 h/d or 24 h/d for 1, 2, 3, 4, 5, 7, 10, 14, 21, 28, 32 or 35 d. Groups of 4-6 rats were killed immediately after exposure and examined for macroscopic abnormalities. Tissues were preserved for histological examination and blood samples were taken to determine testosterone levels. One group of rats following 32 d of exposure were removed from exposure and allowed to recover for 6 d prior to sacrifice.
Following continuous exposure over 35 d resulted in tubules almost empty of spermatids and spermatozoa. Intermittent exposure had milder effects typified by a reduction in the number of spermatogenic cells. Following 6 d without exposure there was clear evidence of recovery of spermatogenesis but in some animals the recovery took longer. There was no effect on testosterone level which was consistent with the lack of any histological changes in interstitial cells.
In conclusion, continual exposure to N2O resulted in depletion of spermatids and spermatozoa, which was reversible upon cessation of exposure. No histopathological changes were present in interstitial cells, with testosterone levels remaining unaffected.
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