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Diss Factsheets
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EC number: 228-601-5 | CAS number: 6303-21-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Skin irritation / corrosion
Administrative data
- Endpoint:
- skin corrosion: in vitro / ex vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- from 2013/11/22 to 2013/12/19
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP study following OECD guideline without deviation
Data source
Reference
- Reference Type:
- other company data
- Title:
- Unnamed
- Year:
- 2 013
- Report date:
- 2013
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 435 (In Vitro Membrane Barrier Test Method for Skin Corrosion)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- 2012/11/30
Test material
- Reference substance name:
- Phosphinic acid
- EC Number:
- 228-601-5
- EC Name:
- Phosphinic acid
- Cas Number:
- 6303-21-5
- Molecular formula:
- H3O2P
- IUPAC Name:
- phosphinic acid
- Test material form:
- other: 50% aqueous solution of Hypophosphorous acid
Constituent 1
Test animals
- Species:
- other: Biobarrier membrane (reconstituted collagen matrix)
- Strain:
- not specified
Test system
- Amount / concentration applied:
- 500 µL
- Duration of treatment / exposure:
- 240 minutes
- Observation period:
- The vials were observed continuously for the first 10 minutes, and at approximate 5 minute intervals for 240 minutes (Category 1 test items) or until breakthrough of the test item had occurred.
- Details on study design:
- Negative Control: Citric Acid, prepared as a 10% w/v solution
+ Batch: 117130
+ Purity: 99.5%
+ Expiry Date: 15 March 2015
+ Storage Conditions: room temperature
Positive Control: Sulphuric Acid
+ Batch: 1331156
+ Purity: >95%
+ Expiry Date: 15 March 2018
+ Storage Conditions: room temperature
Qualification Screen
The qualification screen was used to determine whether the assay was suitable for the test item. For the qualification screen 150 μL of the test item was added to the "Qualify" test tube. If the test item failed to produce a color or physical change in the qualifying test within five minutes, it cannot be analyzed with the CORROSITEX®.
Categorization Screen
The categorization screen was used to enable the test item to be measured against the appropriate classification scale. Test items having high acid/alkaline reserves are defined as ‘Category 1’ items, while those with low acid/alkaline reserves are defined as ‘Category 2’ items.
The screen was performed by adding 150 μL of the test item to each tube (A and B). Each tube was agitated and the resulting colours observed. The categorisation kit and colour chart provided by In Vitro International were used to determine the category.
Biobarrier Preparation
The membrane discs were prepared three days prior to the assay and were refrigerated at 2 to 8 °C overnight before use. Biobarriers are stable for seven days if wrapped and stored at 2 to 8 °C.
A scintillation vial containing the biobarrier matrix powder was placed in a water bath on a hot plate set at 68 to 70 °C, with the stir switch set to maintain a steady rate for the stir-bar. The entire contents of the biobarrier diluent vial were added slowly to the biobarrier matrix powder to ensure complete and uniform solubilisation. The solution was warmed to 68 to 70 °C to solubilise the biobarrier matrix.
Results and discussion
In vitro
Results
- Irritation / corrosion parameter:
- other: other: Breakthrough Time
- Value:
- 12
- Remarks on result:
- other:
- Remarks:
- Basis: other: cells viability. Max. score: 240.0. Reversibility: not reversible. Remarks: the test was stopped after 12 minutes and 5 secondes because of the corrosion effects. (migrated information)
Any other information on results incl. tables
Qualification Screen
The test item produced an immediate color change and therefore qualified for use in the CORROSITEX® assay.
Categorization Screen
The test item was assigned into Category 1
The results of the test:
Vial 1 |
Vial 2 |
Vial 3 |
Vial 4 |
|
Start time (hr:min:sec) |
00:00:00 |
00:04:00 |
00:05:00 |
00:06:00 |
Detection time (hr:min:sec) |
00:12:00 |
00:17:30 |
00:16:30 |
00:17:20 |
Breakthrough time (hr:min:sec) |
00:12:00 |
00:13:30 |
00:11:30 |
00:11:20 |
Mean Breakthrough time of the four test item replicates: 12 minutes 05 seconds
Positive/Negative Controls Quality Criteria
The results of the assay were accepted on condition of adherence to the positive and negative control item ranges given in the following table:
|
Chemical |
Concentration (weight %) |
Breakthrough Time (hr:min:sec) |
Positive control |
Sulphuric acid |
95-98 |
00:01:08 |
Negative control |
Citric Acid |
10 |
No breakthrough observed |
Applicant's summary and conclusion
- Interpretation of results:
- Category 1B (corrosive)
- Remarks:
- Migrated information Criteria used for interpretation of results: EU
- Conclusions:
- The test item was considered to be CORROSIVE. The following classification criteria apply:
UNITED NATIONS PACKING GROUP II.
EU CLP code H314 hazard category 1B - Executive summary:
The Corrositex® assay is a standardized and quantitative in vitro corrosivity test. The potential corrosivity of the test item was assessed by measuring the time that is required for the test item to pass through a biobarrier membrane and produce a change in a Chemical Detection System (CDS).
4 vials of 50% Hypophosphorous acid solution used in the test. According to the results (average breakthrough point: 12 min 05 sec) the substance is considered to be CORROSIVE. The following classification criteria apply:
UNITED NATIONS PACKING GROUP II.
EU CLP code H314 hazard category 1B
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