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EC number: 219-949-9 | CAS number: 2580-78-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1974
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 974
- Report date:
- 1974
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- GLP compliance:
- no
- Test type:
- standard acute method
- Limit test:
- yes
Test material
- Reference substance name:
- 2-(3-(4-amino-9,10-dihydro-3-sulpho-9,10-dioxoanthracen-4-yl)aminobenzenesulphonyl)vinyl) disodium sulphate
- EC Number:
- 219-949-9
- EC Name:
- 2-(3-(4-amino-9,10-dihydro-3-sulpho-9,10-dioxoanthracen-4-yl)aminobenzenesulphonyl)vinyl) disodium sulphate
- Cas Number:
- 2580-78-1
- Molecular formula:
- C22H18N2O11S3.2Na C22H18N2Na2O11S3
- IUPAC Name:
- disodium 1-amino-9,10-dioxo-4-[(3-{[2-(sulfonatooxy)ethyl]sulfonyl}phenyl)amino]-9,10-dihydroanthracene-2-sulfonate
- Test material form:
- solid: particulate/powder
- Details on test material:
- Reactive Blue 19
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Hoechst AG
- Age at study initiation: NA
- Weight at study initiation: 82 - 108 g (mean: 95 g)
- Fasting period before study: 16 hours prior and 2 hours after gavage
- Housing: in groups
- Diet (e.g. ad libitum): ALTROMlN 1324 ad libitum
- Water (e.g. ad libitum): tap water ad libitum
- Acclimation period: NA (own breeding)
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- water
- Details on oral exposure:
- 25 g/mL in deionized water
- Doses:
- 6300, 8000, 10000, 12500 mg/kg bw
- No. of animals per sex per dose:
- 10 females
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations: frequently on day 1, twice daily thereafter
- Frequency of weighing: weekly
- Necropsy of died animals performed: yes
- Other examinations performed: clinical signs, body weight - Statistics:
- Probit analysis according to LINDER and WEBER
Results and discussion
Effect levels
- Key result
- Sex:
- female
- Dose descriptor:
- LD50
- Effect level:
- 10 135 mg/kg bw
- Based on:
- test mat.
- 95% CL:
- 9 355 - 10 979
- Remarks on result:
- other: p = 0.05
- Mortality:
- 6300 mg/kg: 0/10
8000 mg/kg: 1/10
10000 mg/kg: 3/10
12500 mg/kg:10/10
Deaths occurred between 30 minutes and 12 hours after gavage - Clinical signs:
- other: Died animals showed the following signs prior to death: ataxia, prone position, lateral position Visible skin was bluish discolored in all treated animals After 24 hours: all surviving rats: NAD
- Gross pathology:
- died animals: bluish discolored inner organs
- Other findings:
- The dye was excreted via urine and feces.
Any other information on results incl. tables
Dose [mg/kg bw] |
Mortality |
6300 |
0/10 |
8000 |
1/10 |
10000 |
3/10 |
12500 |
10/10 |
Applicant's summary and conclusion
- Interpretation of results:
- practically nontoxic
- Remarks:
- Migrated information Criteria used for interpretation of results: EU
- Conclusions:
- LD50: 10135 mg/kg bw
- Executive summary:
The dye, which is present as a dark blue powder, was administered as an aqueous solution in different doses once by gavage to female SPF Wistar rats of own breeding weighing 82 - 108 g (average weight 95 g). The test was performed in female rats, as sex-related differences could not be determined in preliminary tests. Ten rats were used per dose. The rats were deprived of food 16 hours before application. 2 hours after administration of the dye, the rats were given food again. The observation period after application was 14 days. During this period, in which the animals were weighed weekly, the animals were fed the husbandry diet ALTROMIN 1324 from the company Altrogge in Lage/Lippe and tap water. Feed and water were offered ad libitum. The animals were kept in plastic cages on wood shavings. Lethally poisoned animals were dissected and macroscopically assessed.
The LD50 was determined by means of a probit analysis (method according to LINDER and WEBER); the confidence limits were calculated according to CAVALLI-SFORZA (Dept. of Practical Mathematics of Hoechst Aktiengesellschaft).
Fatally poisoned animals died, after showing ataxia, in prone or lateral position. The hairless parts of the body were coloured blue. The dye was excreted with the faeces and urine. The body weight development of individual animals in the 10000 mg/kg bw group was delayed. The necropsy of the dead animals showed macroscopically a blue colouration of the internal organs.
Acute oral toxicity testing revealed an LD50 of 10135 mg/kg body weight. According to the usual classification based on acute toxicity (W.S. SPECTOR in the "Handbook of Toxicology"), the present dye would be considered practically non-toxic after a single oral administration.
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