Dodecene, branched

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Comparable to guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1989

Report date:

1989

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

Principles of method if other than guideline:

Method: BASF Test

GLP compliance:

no

Test type:

standard acute method

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Dodecene, branched

EC Number:

306-479-5

EC Name:

Dodecene, branched

Cas Number:

97280-83-6

Molecular formula:

C12 H24

IUPAC Name:

(2Z)-4-methylundec-2-ene

Details on test material:

- Name of test material (as cited in study report): Isododecene

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Dr. K. Thomae GmbH, 7950 Biberach, FRG
- Age at study initiation: young adult animals
- Weight at study initiation: comparable weight (+/- 20% of the mean weight)
mean weight of the males: 188 g; mean weight of the females: 187 g
- Housing: 5 animals per cage, stainless steel wire mesh cages (type DK-III, without bedding)
- Diet (e.g. ad libitum): ad libitum (KLIBA laboratory diet), the animals were given no feed about 16 hours before administration
- Water (e.g. ad libitum): ad libitum
- Acclimation period: at least 1 week

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 20 -24°C
- Humidity (%): 30 - 70%
- Photoperiod (hrs dark / hrs light): 12/12
There were no deviations from these ranges which influcenced the results of the study

Animal identification: Identification of groups using cage cards

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

CMC (carboxymethyl cellulose)

Details on oral exposure:

VEHICLE
(0.5 % aqueous carboxymethyl cellulose)
- Concentration in vehicle: 20 g/100 ml
- Administration volume (if gavage): 10 ml/kg
- Justification for choice of vehicle: aqueous formulation corresponds to the physiological medium

Doses:

2000 mg/kg

No. of animals per sex per dose:

5

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: Recording of signs and symptoms occured several times on the day of administration and at least
once each workday; (check for moribund and dead animals twice each workday and once on holidays)
- Necropsy of survivors performed: yes
- Other examinations performed:
determination of body weight: at the beginning of the study and on d 7 and d 13.

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 2 000 mg/kg bw

Mortality:

All animals survived.

Clinical signs:

other: No abnormalities were observed.

Gross pathology:

No pathological findings were noted.