Registration Dossier
Registration Dossier
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EC number: 291-103-1 | CAS number: 90341-71-2
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
Description of key information
The test for growth inhibition on algae according to the EU-guideline, was supplemented by a modified algae test which takes into account the light absorption of the dyes (modified algae test according to Memmert & Knoell (1992)). For both tests, test item concentrations of nominally 12.5, 25, 50, and 100 mg/L were used. With this method, it is possible to differentiate algicidic from algistatic effects.
The concentration response curves obtained in experimental parts A and B the growth rate are considered identical. Hence, the growth inhibition observed is caused by the light absorption of the dye stuff. The substance is not toxic for algae.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 100 mg/L
- EC10 or NOEC for freshwater algae:
- 100 mg/L
Additional information
The test for growth inhibition on algae according to the EU-guideline, was supplemented by a modified algae test which takes into account the light absorption of the dyes (modified algae test according to Memmert & Knoell (1992)). For both tests, test item concentrations of nominally 12.5, 25, 50, and 100 mg/L of a structural analogue were used. With this method, it is possible to differentiate algicidic from algistatic effects.
Investigation of the effects of light absorption of the dyestuff indicates that effects noted in the algal study are due to light absorption rather than associated toxicity. On this basis the substance is not deemed to be toxic to algae. No classification and labelling is applicable.
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