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EC number: 212-298-1 | CAS number: 778-94-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 02 Jan - 26 Feb 2007
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 007
- Report date:
- 2007
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 2-nitro-4-(trifluoromethyl)benzonitrile
- EC Number:
- 212-298-1
- EC Name:
- 2-nitro-4-(trifluoromethyl)benzonitrile
- Cas Number:
- 778-94-9
- Molecular formula:
- C8H3F3N2O2
- IUPAC Name:
- 2-nitro-4-(trifluoromethyl)benzonitrile
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic (adaptation not specified)
- Details on inoculum:
- - Type: Mixed population of aquatic microorganisms (activated sludge)
- Source of inoculum/activated sludge: Aeration tank of a wastewater plant treating predominantly domestic sewage (Wupper area water authority, STP Odenthal). Date of collection: 2007-01-02
- Storage conditions: The inoculum was stored at room temperature under continuous shaking with aeration until use.
- Storage length: The inoculum was stored for max. 3 days.
- Pretreatment: The sludge was washed twice by adding mineral medium, centrifuging (10 min at 2000 rpm and 20 °C) and decanting off the supernatant. 1.135 g of the wet sludge was dried in order to calculate the amount of wet sludge to achieve a concentration of activated sludge of 3 g/L (dry wet) suspended solids. The calculated amount of sludge was first dissolved in mineral medium and then filled up to a defined end volume.
- Concentration of sludge: 30 mg/L ss - Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 100 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: The mineral medium was prepared from stock solutions of mineral components described as follows:
1. Mineral salt solution: Potassium dihydrogen phosphate (KH2PO4) 85.0 g, Dipotassium hydrogenphosphate (K2HPO4) 217.5 g, Disodium hydrogenphosphate dihydrate (Na2HPO4 * 2H2O) 334.0 g, Ammonium chloride (NH4Cl) 5.0 g, The components were dissolved in deionized water and filled up to 10 litres. The pH of the solution was adjusted to 7.4
2. Magnesium sulphate solution: Magnesium sulphate heptahydrate (MgSO4 * 7H2O) 22.5 g, The substrate was dissolved in deionized water and filled up to 1 litre.
3. Calcium chloride solution: Calcium chloride dehydrate (CaCl2 * 2H2O) 36.4 g. The substance was dissolved in deionized water and filled up to 1 litre.
4. Iron (III) chloride solution: Iron (III) chloride hexahydrate (FeCl3 * 6H2O) 0.25 g. The substance was dissolved in deionized water and filled up to 1 litre.
To prepare the mineral medium 10 mL of the mineral salt solution were mixed with 800 mL deionized water. Then 1 mL each of the magnesium sulphate-, calcium chloride- and the iron (III) chloride solution were added and filled up to 1 litre with deionised water.
- Preparation of test flasks with test item: 25 mg of the test item were weighted out on aluminium foil. Test item and aluminium foil were added to the test flasks, which had already been filled with 200 mL of mineral medium. Afterwards the flask content was made up to 250 mL with mineral medium containing inoculum to give a test concentration of 100 mg test item/L.
- Test temperature: 22 ± 1 °C
- pH: 7.4
- pH adjusted: Only the pH of mineral salt medium was adjusted.
- Continuous darkness: yes
TEST SYSTEM
- Culturing apparatus: Oxi Top System (WTW)
- Test volume: 250 mL
- Number of culture flasks/concentration: 2
- Method used to create aerobic conditions: One magnetic stirrer in each test vessel.
- Measuring equipment: Continous Flow Analyser SKALAR SAN Plus System
- Other: Degradation was followed by the determination of oxygen uptake and measurements were taken at frequent intervals to allow the identification of the beginning and end of biodegradation.
Oxygen uptake: The oxygen uptake was calculated from the readings at regular and frequent intervals, using the method given by the manufacturer of the equipment.
Other measurements: At the end of incubation, the pH of the contents of the flasks was measured.
SAMPLING
- Sampling frequency: daily
- Sampling method: Determination of nitrite nitrogen and nitrate nitrogen and the sum of both by flow analysis (CFA and FIA) and spectrometric detection using a Continuous Flow Analyser SKALAR SAN Plus System
CONTROL AND BLANK SYSTEM
- Inoculum blank: Inoculum blank was carried out in triplicate samples.
- Toxicity control: Toxicity control was carried out in one replicate.
- Reference substance: A positive control was carried out in duplicate samples.
Reference substance
- Reference substance:
- benzoic acid, sodium salt
- Remarks:
- 100 mg reference compound/L
Results and discussion
% Degradation
- Parameter:
- % degradation (O2 consumption)
- Value:
- 0
- Sampling time:
- 28 d
- Details on results:
- Within 28 days, a degradation of 0.0% was determined for 2-nitro-4-(trifluoromethyl)benzonitrile. 2-nitro-4-(trifluoromethyl)benzonitrile is considered to be "Not Readily Biodegradable".
BOD5 / COD results
- Results with reference substance:
- The reference compound sodium benzoate showed 82.8% degradation after 14 days and 85.7% degradation after 28 days.
Any other information on results incl. tables
Table 1: Degradation of test item
Time (d) |
Corrected BOD test item (a-bm) (mg O2/L) |
% degradation of test item |
% degradation mean of test item |
% degradation mean of reference compound |
% degradation mean of toxicity control |
||
a1 |
a2 |
a1 |
a2 |
||||
0 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
1 |
0.5 |
0.0 |
0.6 |
0.5 |
0.3 |
30.1 |
3.0 |
2 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
51.2 |
37.8 |
3 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
62.9 |
47.3 |
4 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
68.3 |
49.3 |
5 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
72.0 |
51.0 |
6 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
74.4 |
52.4 |
7 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
76.6 |
52.4 |
8 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
79.0 |
53.3 |
9 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
79.7 |
54.1 |
10 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
80.9 |
54.3 |
11 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
81.5 |
55.3 |
12 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
81.8 |
55.4 |
13 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
82.4 |
55.4 |
14 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
82.8 |
55.8 |
15 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
82.8 |
56.2 |
16 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
83.4 |
56.0 |
17 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
82.8 |
56.0 |
18 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
83.7 |
56.0 |
19 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
83.5 |
56.7 |
20 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
84.1 |
56.5 |
21 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
84.1 |
57.7 |
22 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
83.5 |
57.3 |
23 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
84.7 |
57.7 |
24 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
84.4 |
57.5 |
25 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
85.0 |
57.9 |
26 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
85.4 |
58.5 |
27 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
84.8 |
58.5 |
28 |
0.0 |
0.0 |
0.0 |
0.0 |
0.0 |
85.7 |
58.8 |
Table 2: Validity criteria for OECD 301.
Criterion from the guideline |
Outcome |
Validity criterion fulfilled |
Difference of extremes of replicate values of the removal of the test chemical at the plateau, at the end of the test or at the end of the 10-d window, as appropriate, is less than 20%. |
At the end of the test, biodegradation (%) in parallels with test item did not differ by more than 20%. |
yes |
Percentage degradation of the reference compound reached the pass level by day 14 (≥ 60%). |
Percentage degradation of the reference compound reached with 82.8% degradation the pass level by day 14 (≥ 60%). |
yes |
The toxicity control should degrade to at least 35% (based on DOC) or at least 25% (based on ThOD or ThCO2) within 14 d. |
The degradation in the toxicity control was 55.8% at day 14. |
yes |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- see Table 2 in "Any other information on results incl. tables".
- Interpretation of results:
- under test conditions no biodegradation observed
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