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EC number: 202-269-1 | CAS number: 93-70-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1990-05-22
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Study documented and performed according to GLP standards and in compliance with Directive 84/449/EC, Part B, Method B5.
Cross-reference
- Reason / purpose for cross-reference:
- reference to same study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 990
- Report date:
- 1990
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.5 (Acute Toxicity: Eye Irritation / Corrosion)
- Deviations:
- not applicable
- Principles of method if other than guideline:
- Not applicable.
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 2'-chloroacetoacetanilide
- EC Number:
- 202-269-1
- EC Name:
- 2'-chloroacetoacetanilide
- Cas Number:
- 93-70-9
- Molecular formula:
- C10H10ClNO2
- IUPAC Name:
- 2'-chloroacetoacetanilide
- Details on test material:
- Sample designation: P0005
Physical state: white powder
Storage: ambient temperature
Constituent 1
Test animals / tissue source
- Species:
- rabbit
- Strain:
- New Zealand White
- Details on test animals or tissues and environmental conditions:
- Three New Zealand White strain rabbits in the weight range 2.0 to 2.5 kg, prior to treatment on Day 1 and approximately 9 to 11 weeks of age were
obtained from Froxfield Farms (U.K.) Ltd., Petersfield, Hampshire and A. Smith, Warlinghani, Surrey, England.
The rabbits selected for the study were all acclimated to the laboratory environment.
The rabbits were individually housed in metal cages with perforated floors in Building R 14 Rooms 1 and 4. They had free access to tap water and SDS Standard Rabbit Diet.
Aninial room temperature was maintained at approximately 19°C and relative humidity at 30—70%.
Air exchange was maintained at approximately 19 air changes per hour and lighting was controllecl to give 12 hours of artificial light (0700—1900
hours) in each 24 hour period.
Test system
- Vehicle:
- unchanged (no vehicle)
- Controls:
- yes, concurrent no treatment
- Amount / concentration applied:
- Approximately 89 mg of test item, the weight occupying a volume of 0.1 ml
- Duration of treatment / exposure:
- The eyelids were gently held together for one second before releasing.
- Observation period (in vivo):
- Examination of the eyes was made after 1 hour and 1, 2, 3, 4 and 7 days after instillation. Observation of the eyes was aided by the use of
a handheld torch. - Number of animals or in vitro replicates:
- 3
- Details on study design:
- The eyes of each animal were examined prior to instillation of the test substance to ensure that there was no pre-existing corneal damage or
conjunctival inflammation.
Approximately 89 mg of P0005, the weight occupying a volume of 0.1 ml, was placed into the lower everted lid of one eye of each animal.
The eyelids were then gently held together for one second before releasing. The contralateral eye remained untreated and served as a control.
Results and discussion
In vivo
Resultsopen allclose all
- Irritation parameter:
- cornea opacity score
- Basis:
- mean
- Time point:
- other: 1, 24 , 48, 72 hours and 7 day
- Score:
- 0
- Irritation parameter:
- iris score
- Basis:
- mean
- Time point:
- other: 1, 24 , 48, 72 hours and 7 day
- Score:
- 0
- Irritation parameter:
- conjunctivae score
- Basis:
- mean
- Time point:
- other: 1, 24 , 48, 72 hours and 7 days
- Score:
- 3
- Max. score:
- 3
- Reversibility:
- fully reversible within: 48h and 72h
- Irritant / corrosive response data:
- The numerical scores awarded to the ocular reactions elicited by the test item are given under "any other information on results incl. tables".
- Other effects:
- No corneal damage or iridial inflammation was seen throughout the observation period.
Mild conjunctival inflammation was evident in all three animals. All reactions had resolved two or three days after instillation.
Any other information on results incl. tables
Animal No. |
1 |
2 |
3 |
3 hours/days |
1 24 48 72 7 |
1 24 48 72 7 |
1 24 48 72 7 |
Cornea |
|
|
|
E = Degree |
0 |
0 |
0 |
F = Area |
0 |
0 |
0 |
(F x E) x 5 |
0 |
0 |
0 |
D = Iris |
0 |
0 |
0 |
D x 5 |
0 |
0 |
0 |
Conjunctiva |
|
|
|
A = Redness |
1 1 0 0 0 |
1 1 1 0 0 |
1 1 0 0 0 |
B = Chemosis |
0 0 0 0 0 |
1 1 0 0 0 |
1 1 0 0 0 |
C = Discharge |
|
|
|
(A + B+ C) x 2 |
2 2 0 0 0 |
4 4 2 0 0 |
4 4 0 0 0 |
Total |
2 2 0 0 0 |
4 4 2 0 0 |
4 4 0 0 0 |
Applicant's summary and conclusion
- Interpretation of results:
- not irritating
- Remarks:
- Migrated information Criteria used for interpretation of results: OECD GHS
- Conclusions:
- The test item is not irritating to the eye according to the GHS Classification criteria.
- Executive summary:
The experimental procedure complied with EEC Methods for determination of toxicity Directive 84/449/EEC (OJ No. L251, 19.9.84), Part B, Method B5 Acute toxicity (eye irritation).
Three New Zealand White strain albino rabbits were chosen as it has been shown to be a suitable model for eye irritation studies and is the animal recommended in the test protocol.
The test substance was administered as supplied by the Sponsor. Examination of the eyes was made after 1 hour and 1, 2, 3, 4 and 7 days after instillation.
No corneal damage or iridial inflammation was seen throughout the observation period. Mild conjunctival inflammation was evident in all three animals. All reactions had resolved two or three days after instillation.
In conclusion, the test item is not irritating to the rabbit eye and therefore has not been classified according to GHS.
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