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EC number: 429-300-3 | CAS number: 155601-30-2 PYRAZOL P5; PYRAZOLE DHE
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Skin sensitisation
Administrative data
- Endpoint:
- skin sensitisation: in vivo (non-LLNA)
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: According Guideline and GLP
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 995
- Report date:
- 1995
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.6 (Skin Sensitisation)
- GLP compliance:
- yes (incl. QA statement)
- Type of study:
- guinea pig maximisation test
Test material
- Reference substance name:
- 1-(2-hydroxyethyl)-1H-pyrazol-4,5-diyldiammoniumsulfate
- EC Number:
- 429-300-3
- EC Name:
- 1-(2-hydroxyethyl)-1H-pyrazol-4,5-diyldiammoniumsulfate
- Cas Number:
- 155601-30-2
- Molecular formula:
- C5H12N4O5S
- IUPAC Name:
- 2-(4,5-diamino-1H-pyrazol-1-yl)ethan-1-ol; sulfuric acid
- Details on test material:
- Batch No GSF 4-20079
Constituent 1
In vivo test system
Test animals
- Species:
- guinea pig
- Strain:
- Hartley
- Sex:
- female
Study design: in vivo (non-LLNA)
Inductionopen allclose all
- Route:
- intradermal
- Vehicle:
- petrolatum
- Concentration / amount:
- intradermal concentrations were 10%, 1%, 0.1% and 0.01% in physiological saline
epicutaneous exposure with concentrations of 40%, 10%, 2.5% and 0.5% test substance in white petrolatum.
Challengeopen allclose all
- Route:
- epicutaneous, occlusive
- Vehicle:
- petrolatum
- Concentration / amount:
- intradermal concentrations were 10%, 1%, 0.1% and 0.01% in physiological saline
epicutaneous exposure with concentrations of 40%, 10%, 2.5% and 0.5% test substance in white petrolatum.
Results and discussion
Any other information on results incl. tables
No adverse skin reactions were noted on the control sites treated with the vehicle, neither in the conrtol group nor in the test substance grpup. The test substance treated sites were stained orange-red. This probably obscured the reading of very slight to well defined erythema. Therefore a histopathological examination of theses aereas were perfomed.Results of visual and histopathological examinations were combiened to assess the sensitising potential of the test substance.
Applicant's summary and conclusion
- Interpretation of results:
- sensitising
- Remarks:
- Migrated information
- Conclusions:
- based on the outcome of this study the test substance "DA 010894" Pyrazole DHE should classified as "May cause sensitisation by skin contact".
- Executive summary:
The maximisation test according to the EEC guideline B6 was perfomed to reveal a possible sensitising potential of "DA 10894" Pyrazole DHE. 10 female guinea pigs were used as a test substance grooup and another 5 females were used as a negative contraol group.There were two induction exposes (intradermally and epicutaneously) and one epicoutaneous challange expoure.
Test substance concentrations were
- 1% in physiological saline for the intradermal induction
- 40% in white petrolatum for the epicutaneous induction and
- 40% in white petrolatum for the challenge exposure
results:
All animals survived.
No adverse skin reactions were noted on the control sites treated with the vehicle, neither in the conrtol group nor in the test substance grpup. The test substance treated sites were stained orange-red. This probably obscured the reading of very slight to well defined erythema. Therefore a histopathological examination of theses aereas were perfomed.Results of visual and histopathological examinations were combiened to assess the sensitising potential of the test substance.
In the test substance group, 10/10 animals , i.e. 100% of the animals , were regarded as sensitised. As no animal of the negative control group had a positive skin reaction, this was also in the net rate of the sensitised animals in the test substance group.
Therefore, based on the outcome of this study the test substance "DA 010894" Pyrazole DHE should classified as "May cause sensitisation by skin contact".
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