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EC number: 200-846-2 | CAS number: 75-18-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Description of key information
Dimethyl sulphide was found to be not irritating to skin and slightly irritating to eyes in rabbits.
Skin irritation:
The potential corrosive and irritant properties of Dimethyl Sulphide, following dermal application on rabbits was evaluated in a study conducted in compliance with the OECD Guideline No. 404 (Silvano, 2015 c). Dimethyl Sulphide was first applied for periods of 3 minutes, 1 hour and 4 hours to a single male New Zealand White rabbit. After the 4-hour application, since the mean value from grading at 24, 48 and 72 hours after patch removal was < 2.3 for erythema and for edema, the test item was applied on the skin of two other animals for 4 hours. A dosage-volume of 0.5 mL/flank was used. The test item was placed on a gauze pad, which was then applied to a skin area of approximately 6 cm2. The gauze pad was held in place by a non-irritation semi-occlusive dressing and a restraining bandage.After required period of contact with the skin, the dressing was removed. Each animal was observed once a day for mortality and clinical signs. For each exposure period, cutaneous reactions were evaluated approximately 1, 24, 48 and 72 hours after removal of the dressing. The mean values of the scores for erythema and edema were calculated for each animal. Body weight was recorded on the day of treatment and at the end of the evaluation of cutaneous reactions.
No unscheduled deaths occurred during the study and no clinical signs were noted in any animals. The body weight of the animals was unaffected by the test item treatment. No cutaneous reactions were observed during the study. Mean scores calculated for each animal over 24, 48 and 72 hours were as follows:
. erythema: 0.0, 0.0, 0.0; showing no significant inflammation,
. edema: 0.0, 0.0; 0.0; showing no significant inflammation.
Dimethyl Sulphide was non-irritant when applied topically to rabbits.
Eye irritation:
The potential eye irritant properties of Dimethyl Sulphide, following a single ocular administration to rabbits was evaluated in a study conducted in compliance with the OECD guideline No. 405 (Silvano, 2015d). Dimethyl Sulphide was first administered to a single male New Zealand White rabbit. As mean value from grading at 24, 48 and 72 hours after instillation was [0-2] for conjunctival edema (chemosis) and for conjunctival redness, [0 -1] for iris lesions and for corneal opacity, the test item was administered in the left eye of two other animals. The test item was administered inthe conjunctival sac of the left eye. The right eye remained untreated and acted as control. A dosage-volume of 0.1 mL/animal was used. For the first animal, a local anesthetic was used prior to treatment and the animals were placed under systemic analgesia throughout the study. The eyes were not rinsed before the 24-hour scoring. Each animal was observed once a day for mortality and clinical signs. Ocular reactions were observed approximately 1, 24, 48 and 72 hours after the administration. The mean values of the scores for chemosis, redness of the conjunctiva, iris lesions and corneal opacity were calculated for each animal. Body weight was recorded on the day of treatment and at the end of the evaluation of ocular reactions (Day 4). On completion of the observation period, the animals were sacrificed then discarded without macroscopic post-mortem examination.
No unscheduled deaths occurred during the study and no clinical signs were noted in any animals.The body weight of the animals was unaffected by the test item treatment.In the left treated eye of the first animal, marked chemosis and severe redness of the conjunctiva were recorded 1 hour after test item administration. Chemosis became slight and redness was moderate on Day 2. No iris and corneal lesions were observed. In the left treated eye of the second and third animals, marked chemosis and moderate redness of the conjunctiva were recorded 1 hour after test item administration. Chemosis became moderate on Day 2 and then slight on Day 3. Moderate redness persisted on Day 3 for the third animal or on Day 2 for the second animal, becoming slight on Day 3. No iris and corneal lesions were observed.
Mean scores calculated for each animal over 24, 48 and 72 hours were as follows:
chemosis: 0.3, 1.0 and 1.0,
redness of the conjunctiva: 0.7, 1.0 and 1.3,
iris lesions: 0.0, 0.0 and 0.0,
corneal opacity: 0.0, 0.0 and 0.0.
Dimethyl Sulphidewas slightly irritant when administered by ocular route to rabbits.
Key value for chemical safety assessment
Skin irritation / corrosion
Link to relevant study records
- Endpoint:
- skin irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 404 (Acute Dermal Irritation / Corrosion)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Species:
- rabbit
- Strain:
- New Zealand White
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: CEGAV, Argenvilliers, France.
- Age at study initiation: 3 to 4 months old
- Weight at study initiation: 3130 g to 3510 g
- Housing: individually housed in noryl cages
- Diet: pelleted breeding diet "type 110C", ad libitum
- Water:. ad libitum
- Acclimation period: at least 7 days
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 18 ± 3
- Humidity (%): 50 ± 20
- Air changes (per hr): 5 to 15
- Photoperiod (hrs dark / hrs light): 12h/12h - Type of coverage:
- semiocclusive
- Preparation of test site:
- clipped
- Vehicle:
- unchanged (no vehicle)
- Controls:
- not required
- Amount / concentration applied:
- 0.5 ml
- Duration of treatment / exposure:
- 3-minute, 1- and 4-hour
- Observation period:
- 1 hour, 24, 48 and 72 hours
- Number of animals:
- 3
- Details on study design:
- TEST SITE
- Area of exposure: right and left anterior and/or posterior flanks
- coverage: 6 cm²
- Type of wrap if used: semi-occlusive dressing and restraining bandage
REMOVAL OF TEST SUBSTANCE
- Washing: no
SCORING SYSTEM:
Dermal irritation was graded in each animal according to the following scoring scale:
Erythema and eschar formation:
. no erythema: 0
. very slight erythema (barely perceptible): 1
. well-defined erythema: 2
. moderate to severe erythema: 3
. severe erythema (beet redness) to slight eschar formation (injuries in depth): 4
Edema formation
. no edema: 0
. very slight edema (barely perceptible): 1
. slight edema (edges of area well-defined by definite raising) : 2
. moderate edema (raised by approximately 1 millimeter): 3
. severe edema (raised by more than 1 millimeter and extending beyond area
of exposure): 4
STUDY DESIGN
Rationale for design selection
The sequential study design was as follows:
The test item was applied for 3 minutes on the skin of a single animal (D31251):
¿ as no full thickness destruction of skin tissue was observed in the first hour after removal of the pad, the test item was applied on another treatment site for 1 hour.
After the 1-hour application on animal D31251:
¿ as no full thickness destruction of skin tissue was observed in the first hour after removal of the pad, the test item was applied on another treatment site for 4 hours.
After the 4-hour application on animal D31251:
¿ as mean value from grading at 24, 48 and 72 hours after patch removal was < 2.3 for erythema and for edema, the test item was applied on the skin of two other animals for 4 hours (D31252 and D31253).
For each animal, the day of dose application was recorded as Day 1 of its observation period. - Irritation parameter:
- erythema score
- Basis:
- animal: #1, 2 and 3
- Time point:
- other: 24, 48 and 72 hours
- Score:
- 0
- Max. score:
- 4
- Irritation parameter:
- edema score
- Basis:
- animal: #1, 2 and 3
- Time point:
- other: 24, 48 and 72 hours
- Score:
- 0
- Max. score:
- 4
- Irritant / corrosive response data:
- No cutaneous reactions were observed during the study.
Mean scores calculated for each animal over 24, 48 and 72 hours were as follows:
¿ erythema: 0.0, 0.0, 0.0; showing no significant inflammation,
¿ edema: 0.0, 0.0; 0.0; showing no significant inflammation. - Other effects:
- MORTALITY
No unscheduled deaths occurred during the study.
BODY WEIGHT
The body weight of the animals was unaffected by the test item treatment. - Interpretation of results:
- GHS criteria not met
- Conclusions:
- Dimethyl Sulphide was non-irritant when applied topically to rabbits.
- Executive summary:
The potential corrosive and irritant properties of Dimethyl Sulphide, following dermal application on rabbits was evaluated in a study conducted in compliance with the OECD Guideline No. 404. Dimethyl Sulphidewas first applied for periods of 3 minutes, 1 hour and 4 hours to a single male New Zealand White rabbit. After the 4-hour application, since the mean value from grading at 24, 48 and 72 hours after patch removal was < 2.3 for erythema and for edema, the test item was applied on the skin of two other animals for 4 hours. A dosage-volume of 0.5 mL/flank was used. The test item was placed on a gauze pad, which was then applied to a skin area of approximately 6 cm2. The gauze pad was held in place by a non-irritation semi-occlusive dressing and a restraining bandage.After required period of contact with the skin, the dressing was removed. Each animal was observed once a day for mortality and clinical signs. For each exposure period, cutaneous reactions were evaluated approximately 1, 24, 48 and 72 hours after removal of the dressing. The mean values of the scores for erythema and edema were calculated for each animal. Body weight was recorded on the day of treatment and at the end of the evaluation of cutaneous reactions.
No unscheduled deaths occurred during the study and no clinical signs were noted in any animals. The body weight of the animals was unaffected by the test item treatment. No cutaneous reactions were observed during the study. Mean scores calculated for each animal over 24, 48 and 72 hours were as follows:
. erythema: 0.0, 0.0, 0.0; showing no significant inflammation,
. edema: 0.0, 0.0; 0.0; showing no significant inflammation.
Dimethyl Sulphide was non-irritant when applied topically to rabbits.
Reference
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed (not irritating)
Eye irritation
Link to relevant study records
- Endpoint:
- eye irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 405 (Acute Eye Irritation / Corrosion)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Species:
- rabbit
- Strain:
- New Zealand White
- Details on test animals or tissues and environmental conditions:
- TEST ANIMALS
- Source: CEGAV, Argenvilliers, France.
- Age at study initiation: 3 to 4 months old
- Weight at study initiation: 3130 g to 3510 g
- Housing: individually housed in noryl cages
- Diet: pelleted breeding diet "type 110C", ad libitum
- Water:. ad libitum
- Acclimation period: at least 7 days
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 18 ± 3
- Humidity (%): 50 ± 20
- Air changes (per hr): 5 to 15
- Photoperiod (hrs dark / hrs light): 12h/12h - Vehicle:
- unchanged (no vehicle)
- Controls:
- not required
- Amount / concentration applied:
- 0.1 ml
- Duration of treatment / exposure:
- not rinced
- Observation period (in vivo):
- 1, 24, 48 and 72 hours
- Number of animals or in vitro replicates:
- 3
- Details on study design:
- REMOVAL OF TEST SUBSTANCE
- Washing : no - Irritation parameter:
- chemosis score
- Basis:
- animal #1
- Time point:
- other: 24, 48 and 72 hours
- Score:
- 0.3
- Max. score:
- 4
- Reversibility:
- fully reversible within: 48 hours
- Irritation parameter:
- chemosis score
- Basis:
- other: #1 and 2
- Time point:
- other: 24, 48 and 72 hours
- Score:
- 1
- Max. score:
- 4
- Reversibility:
- fully reversible within: 72 hours
- Irritation parameter:
- conjunctivae score
- Basis:
- animal #1
- Time point:
- other: 24, 48 and 72 hours
- Score:
- 0.7
- Max. score:
- 3
- Reversibility:
- fully reversible within: 48 hours
- Irritation parameter:
- conjunctivae score
- Basis:
- animal #2
- Time point:
- other: 24, 48 and 72 hours
- Score:
- 1
- Max. score:
- 3
- Reversibility:
- fully reversible within: 72 hours
- Irritation parameter:
- conjunctivae score
- Basis:
- animal #3
- Time point:
- other: 24, 48 and 72 hours
- Score:
- 1.3
- Max. score:
- 3
- Reversibility:
- fully reversible within: 72 hours
- Irritation parameter:
- iris score
- Basis:
- animal: #1, 2 and 3
- Time point:
- other: 24, 48 and 72 hours
- Score:
- 0
- Max. score:
- 2
- Irritation parameter:
- cornea opacity score
- Basis:
- animal: #1, 2 and 3
- Time point:
- other: 24, 48 and 72 hours
- Score:
- 0
- Max. score:
- 4
- Irritant / corrosive response data:
- No ocular reactions were observed in the right untreated control eye.
First animal (D31531)
In the left treated eye of male D31531, marked chemosis (grade 3) and severe redness (grade 3) of the conjunctiva were recorded 1 hour after test item administration.
Chemosis became slight (grade 1) and redness became moderate (grade 2) on Day 2.
No iris and corneal lesions were observed.
Second and third animals (D31532 and D31533)
In the left treated eye of males D31532 and D31533, marked chemosis (grade 3) and moderate redness (grade 2) of the conjunctiva were recorded 1 hour after test item administration.
Chemosis became moderate (grade 2) on Day 2 and then slight (grade 1) on Day 3.
Moderate redness (grade 2) persisted on Day 3 for male D31533 or on Day 2 for male D31532, becoming slight (grade 1) on Day 3.
No iris and corneal lesions were observed.
Mean scores calculated for each animal over 24, 48 and 72 hours were as follows:
¿ chemosis: 0.3, 1.0 and 1.0,
¿ redness of the conjunctiva: 0.7, 1.0 and 1.3,
¿ iris lesions: 0.0, 0.0 and 0.0,
¿ corneal opacity: 0.0, 0.0 and 0.0. - Other effects:
- No unscheduled deaths occurred during the study and no clinical signs were noted in any animals. The body weight of the animals was unaffected by the test item treatment.
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- Dimethyl Sulphide was slightly irritant when administered by ocular route to rabbits.
- Executive summary:
The potential eye irritant properties of Dimethyl Sulphide, following a single ocular administration to rabbits was evaluated in a study conducted in compliance with the OECD guideline No. 405. Dimethyl Sulphide was first administered to a single male New Zealand White rabbit. As mean value from grading at 24, 48 and 72 hours after instillation was [0-2] for conjunctival edema (chemosis) and for conjunctival redness, [0 -1] for iris lesions and for corneal opacity, the test item was administered in the left eye of two other animals. The test item was administered inthe conjunctival sac of the left eye. The right eye remained untreated and acted as control.A dosage-volume of 0.1 mL/animal was used.For the first animal, a local anesthetic was used prior to treatment and the animals were placed under systemic analgesia throughout the study. The eyes were not rinsed before the 24-hour scoring. Each animal was observed once a day for mortality and clinical signs. Ocular reactions were observed approximately 1, 24, 48 and 72 hours after the administration. The mean values of the scores for chemosis, redness of the conjunctiva, iris lesions and corneal opacity were calculated for each animal. Body weight was recorded on the day of treatment and at the end of the evaluation of ocular reactions (Day 4). On completion of the observation period, the animals were sacrificed then discarded without macroscopic post-mortem examination.
No unscheduled deaths occurred during the study and no clinical signs were noted in any animals.The body weight of the animals was unaffected by the test item treatment.In the left treated eye of the first animal, marked chemosis and severe redness of the conjunctiva were recorded 1 hour after test item administration. Chemosis became slight and redness was moderate on Day 2. No iris and corneal lesions were observed. In the left treated eye of the second and third animals, marked chemosis and moderate redness of the conjunctiva were recorded 1 hour after test item administration. Chemosis became moderate on Day 2 and then slight on Day 3. Moderate redness persisted on Day 3 for the third animal or on Day 2 for the second animal, becoming slight on Day 3. No iris and corneal lesions were observed.
Mean scores calculated for each animal over 24, 48 and 72 hours were as follows:
chemosis: 0.3, 1.0 and 1.0,
redness of the conjunctiva: 0.7, 1.0 and 1.3,
iris lesions: 0.0, 0.0 and 0.0,
corneal opacity: 0.0, 0.0 and 0.0.
Dimethyl Sulphidewas slightly irritant when administered by ocular route to rabbits.
Reference
The potential eye irritant properties of Dimethyl Sulphide following a single ocular administration to rabbits was evaluated in a study conducted in compliance with the OECD guideline No. 405. Dimethyl Sulphide was first administered to a single male New Zealand White rabbit. As mean value from grading at 24, 48 and 72 hours after instillation was [0-2] for conjunctival edema (chemosis) and for conjunctival redness, [0 -1] for iris lesions and for corneal opacity, the test item was administered in the left eye of two other animals. The test item was administered in the conjunctival sac of the left eye. The right eye remained untreated and acted as control.A dosage-volume of 0.1 mL/animal was used.For the first animal, a local anesthetic was used prior to treatment and the animals were placed under systemic analgesia throughout the study. The eyes were not rinsed before the 24-hour scoring. Each animal was observed once a day for mortality and clinical signs.Ocular reactions were observed approximately 1, 24, 48 and 72 hours after the administration. The mean values of the scores for chemosis, redness of the conjunctiva, iris lesions and corneal opacity were calculated for each animal. Body weight was recorded on the day of treatment and at the end of the evaluation of ocular reactions (Day 4). On completion of the observation period, the animals were sacrificed then discarded without macroscopic post-mortem examination.
No unscheduled deaths occurred during the study and no clinical signs were noted in any animals.The body weight of the animals was unaffected by the test item treatment.In the left treated eye of the first animal, marked chemosis and severe redness of the conjunctiva were recorded 1 hour after test item administration. Chemosis became slight and redness was moderate on Day 2. No iris and corneal lesions were observed. In the left treated eye of the second and third animals, marked chemosis and moderate redness of the conjunctiva were recorded 1 hour after test item administration. Chemosis became moderate on Day 2 and then slight on Day 3. Moderate redness persisted on Day 3 for the third animal or on Day 2 for the second animal, becoming slight on Day 3. No iris and corneal lesions were observed.
Mean scores calculated for each animal over 24, 48 and 72 hours were as follows:
. chemosis: 0.3, 1.0 and 1.0,
. redness of the conjunctiva: 0.7, 1.0 and 1.3,
. iris lesions: 0.0, 0.0 and 0.0,
. corneal opacity: 0.0, 0.0 and 0.0.
Dimethyl Sulphide was slightly irritant when administered by ocular route to rabbits.
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed (not irritating)
Respiratory irritation
Endpoint conclusion
- Endpoint conclusion:
- no study available
Additional information
Justification for classification or non-classification
According to Regulation (EC) No 1272/2008:
Dimethyl sulphide is not irritating to the skin of the rabbit, therefore, no classification is warranted.
Dimethyl sulphide is slightly irritating to the rabbit eye, therefore, no classification is warranted.
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