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Diss Factsheets
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EC number: 605-717-8 | CAS number: 174333-80-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- - Name of test material (as cited in study report): C-SAT 090069
- Physical state: amber coloured viscous liquid
- Analytical purity: > 89%
- Lot/batch No.: C-081
- Expiration date of the lot/batch: 26.02.2013
- Storage condition of test material: room temperature - Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: The test substance was directly weighed into the incubation vessels. - Test organisms (species):
- activated sludge, domestic
- Details on inoculum:
- - Laboratory culture: fresh sample of activated sludge taken from the aeration basin of a municipal sewage treatment plant (Moers, Germany)
- Preparation of inoculum for exposure: Activated sludge was filtered by means of a water jet pump through a filter paper. The sludge was washed twice with mineral medium. Afterwards, 4g/L activated sludge was suspended in mineral medium, 50 mL synthetic sewage were added and the whole mixture was stirred and aerated overnight at 20 °C. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Test temperature:
- 20 ±2 °C
- pH:
- 7.61 - 8.37
- Nominal and measured concentrations:
- Nominal: 10, 32, 100, 320 and 1000 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 1 L beakers (incubation) and 300 mL Karlsruher flasks (O2 measurement)
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 2
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to guideline
EFFECT PARAMETERS MEASURED: Oxygen consumption was followed after the 3h incubation period by continuous measurement of the oxygen concentration with an oxygen electrode for up to 10 min. - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- 200.4 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- - Effect concentrations exceeding solubility of substance in test medium: Test substance was directly weighed into vessels because of its negligible water solubility.
- Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- Validity criteria fulfilled:
- yes
- Conclusions:
- In a GLP study according to OECD guideline 209, the 3h-EC50 of the test substance is > 1000 mg/L, while the respective EC10 is determined to be 200.4 mg/L.
- Executive summary:
The toxicity of the test substance to microorganisms was evaluated in a study according to OECD guideline 209 under GLP conditions.
In a static study domestic activated sludge was exposed for 3h. The test material was applied directly to the test vessels. Analytical monitoring was not performed. All validity criteria were fulfilled. The EC50 is determined to be > 1000 mg/L, while the respective EC10 is determined to be 200.4 mg/L based on nominal concentrations.
Reference
Description of key information
EC50 (3h) > 1000 mg/L, EC10 (3h) = 200.4 mg/L (nominal) for activated sludge (OECD 209)
Key value for chemical safety assessment
- EC10 or NOEC for microorganisms:
- 200.4 mg/L
Additional information
One study investigating the toxicity of the test substance to aquatic microorganisms is available. The study was performed according to OECD guideline 209 under GLP conditions using domestic activated sludge as inocculum (Wehrhahn 2010). The test material was applied directly to the test vessels. Analytical monitoring was not performed. All validity criteria were fulfilled. The EC50 is determined to be > 1000 mg/L, while the respective EC10 is determined to be 200.4 mg/L based on nominal concentrations.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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