Merginol 901

Endpoint summary

Additional information

Toxicity to fish:

The acute toxicity to fish of the test item was investigated in a test according to Directive 92/69/EEC. 10 test fish of the species Brachydanio rerio were exposed to the test item in a 96 h semi-static test with daily change of the test solution. The water accomodated fractions (WAF) of following nominal tested concentrations were applied: 0, 4.27, 9.39, 20.7, 45.5 and 100 mg test substance/L. The test substance was analytical monitored by gas chromatography after extraction and derivation. The LC50 was estimated to be > 100 mg test substance/L (nominal)(Henkel, 1997).

Toxicity to daphnia:

The acute toxicity to invertebrates was determined according to OECD 202 as a limit test since the test item is only partially soluble, the water accommodated fraction (WAF) approach was used. No significant effects (≤10% immobilization out of a total of 40 Daphnia) were observed after 24 and 48 h of exposure. Based on these results, the 24 and 48 h EC50 values of Fatty acids, C16-18 and C18-unsatd., Me esters, epoxidized, reaction products with trimethylolpropane were >100 mg/l (based on loading rate). The no-observed-effect concentrations (NOEC) for 24 and 48 h of exposure were ≥100 mg/l. 100% immobilization was not detected within 48 h of exposure.(BASF, 2012).

The chronic toxicity to invertebrates was determined according to OECD 211 as a limit test with an analogous substance fatty acids, C16-18 and C18 -unsatd., Me esters, epoxidized, reaction products with ethylene glycol (CAS 211450 -67 -3). The test substance is a liquid which is poorly soluble in water. Therefore the test solutions were prepared in order to generate a water accommodated fraction (WAF) of the test substance in test medium. No significant mortality or any other additional significant adverse effects or abnormal behavior were observed in any of the test treatments. Thus, the No Observed Effect (NOEC) concentration was > 10 mg/l. (BASF, 2013)

The study was tested with a similar molecule, which has the same structural body, only the reaction products of the functional groups are different

(instead of reaction products with trimethyolpropane -> reaction products with ethylene glycol). Both substances show similar structural, physico-chemical, environmental and toxicological properties.

Toxicity to algae:

The toxicity the test item to the unicellular freshwater green alga Desmodesmus subspicatus was determined according to the principles of OECD-Guideline 201 and Commission Directive 92/69/EC Method C.3 (1992). The aim of the study was to assess the effects on growth rate and biomass production over a period of 72 h. The study was conducted under static conditions with an initial cell density of nominally 10E03-10E04 cells/mL. A dose response test was carried out with water accommodated fractions (WAF) and 8 loading levels in a geometrical series with a dilution factor of 2, nominal: 1.56 -3.13 -6.25 -12.5 -25 50 -100 -200 mg/L. The preparation method has been chosen with regard to the test item properties and in accordance with the relevant OECD guidance document (2000). Three replicates were tested for the test item loadings and six replicates for the control. Microscopic evaluation of the cells at start of the incubation period revealed no morphological abnormalities. At the end the algae cells were partly agglutinated at loading level 12.5 mg/L. Environmental conditions were determined to be within the acceptable limits. The concentrations of the test item were analysed at ail loading levels at test start and test end via LC MS/MS.

The following results were obtained:

Inhibition of biomass growth: EbL50: 5.93 mg/L; NOEL: 3.13 mg/L;

Rate-related inhibition: ErL50: 20.5 mg/L; NOEL: 6.25 mg/L (Dr. U. Noack-Laboratorien, 2005).

Toxicity to microorganism:

The toxicity of the test item to aquatic microorganisms was tested in the oxygen consumption inhibition test according to DIN 38412, Teil 27. A bacterial suspension of Pseudomonas putida was exposed to a nominal concentration range of 30 to 10000 mg/L of the test item. The test item was directly weighed into the test vessel. The oxygen consumption inhibition was measured in relation to three blank samples. The maximum inhibition value of the test sample was less than 10 % in relation to the blank samples. The EC0 was determined to 10000 mg/L. The EC10 was estimated to be > 10000 mg/L (Henkel, 1996).