Reaction mass of 2-(2-thienyl)ethyl benzenesulphonate and toluene

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Non-GLP, guideline study, published in peer reviewed literature, fully adequate for assessment.

Data source

Reference

Reference Type:

publication

Title:

Modeling acute and chronic toxicity of nonpolar narcotic chemicals and mixtures to Ceriodaphnia dubia.

Author:

Niederlehner BR, Cairns J, Smith EP

Year:

1998

Bibliographic source:

Ecotoxicol. Environ. Saf. 39, 136-146.

Materials and methods

Test guideline

Guideline:

other: US EPA 600/4-91-003

Principles of method if other than guideline:

Modification to minimise volatilisation. Instead of 30ml beakers, individual organisms were tested in 25ml borosilicate glass vials, filled to capacity and closed tightly with teflon PTFE-line silicon septa held in place with poplyproplyene screw on caps.

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Toluene

EC Number:

203-625-9

EC Name:

Toluene

Cas Number:

108-88-3

Molecular formula:

C7H8

IUPAC Name:

toluene

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

Toxicant concentrations were monitored at the start and end of the experiment. Newly prepared test solution and 24hours old test solution were analysed.

Test solutions

Vehicle:

not specified

Test organisms

Test organisms (species):

Ceriodaphnia dubia

Details on test organisms:

Obtained from Aquastar Inc (Mobile, Alabama) and cultured in house.

Study design

Test type:

other: daily renewal

Water media type:

freshwater

Limit test:

no

Total exposure duration:

7 d

Post exposure observation period:

none

Test conditions

Hardness:

Mean 68.3 mg/L

Test temperature:

data not reported

pH:

Mean 7.598

Dissolved oxygen:

> 7 ppm

Salinity:

Not applicable

Nominal and measured concentrations:

Nominal 3, 5, 8, 30, 45uM. Acute results reported as the geometric mean of the measured starting concentrations. Replicates that lost >50 % test substance between renewals were excluded (no info on whether this rule was applied).

Details on test conditions:

Photoperiod 16L:8D. Daphnids fed a mixture of algal cells and ceral leaf at 3E5 algal cells.ml/L and <0.03mg.ml/L cereal solids.

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Duration:

2 d

Dose descriptor:

LC50

Effect conc.:

3.78 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mortality

Details on results:

Only the highest test concentration resulted in mortality, with 60% mortality after 48 hours. 2 day LC50 equals 3.78mg/L.

Reported statistics and error estimates:

99.8 % confidence intervals 29-> 45uM

Applicant's summary and conclusion

Validity criteria fulfilled:

not specified

Conclusions:

The 48 hour EC50 for acute toxicity to aquatic invertebrates is 3.78 mg/l.