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EC number: 220-150-2 | CAS number: 2643-07-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Study well documented, meets generally accepted scientific principles, acceptable for assessment
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 980
- Report date:
- 1980
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 472 (Genetic Toxicology: Escherichia coli, Reverse Mutation Assay)
- Principles of method if other than guideline:
- Method: other according to Ames et al., Mut. Res. 31, 347-364, 1975
- GLP compliance:
- no
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- p-[(2-chloroethyl)ethylamino]benzaldehyde
- EC Number:
- 220-150-2
- EC Name:
- p-[(2-chloroethyl)ethylamino]benzaldehyde
- Cas Number:
- 2643-07-4
- Molecular formula:
- C11H14ClNO
- IUPAC Name:
- 4-[(2-chloroethyl)(ethyl)amino]benzaldehyde
- Details on test material:
- N-Ethyl-N-(2-chlorethyl)-p-amino-benzaldehyd (purity >90%)
Constituent 1
Method
Species / strain
- Species / strain / cell type:
- other: Salmonella tyhpimurium TA 100, TA 1537, TA 98; E.coli WP2 uvrA
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9-Mix prepared from sprangue Dawlay rats after Aroclor 1254 activation
- Test concentrations with justification for top dose:
- 5, 15.8, 50, 158, 500, 1580, 5000 µg/plate
- Details on test system and experimental conditions:
- The mutagenicity experiments with his- S. typhimurium were performed with minor modification of the method described by Ames. The test compound, 500 µl S-9 Mix from Aroclor1254-treated rats (or 500 µl 150 mM KCl), 100 µl of the bacterial suspension and 2000 µl top agar, which consisted of 0.55 % agar, 0.55 % NaCl, 50 µM histidine, 50 µM biotin, and 25 µM Na phosphate pH 7.4, 45°C, were mixed in a test tube and poured onto a
petridish with minimal agar consisting of 1.5 % agar and Vogel-Bonner E medium with 2 % glucose. Where indicated the epoxide hydratase inhibitor
and glutathione depletor, TCPO (2.6 µMol per plate) was added in 10 µl DMSO. Corresponding plates without DMSO received 10 µl DMSO. After
incubation in the dark for 3 days colonies (his+ revertants) were counted.
Expeximents with trp- E. coli were performed the same way, but histidine and biotin of the top agar were replaced by 50 µM tryptophan. Six different concentrations from 15.8 to 5000 µg were used in the experiments for direct mutagenicity. In the experiments with S-9 Mix, seven doses from 5 to 5000 µg were tested.
Every experiment contained positive controls for checking the activity of the metabolizing systemn and the mutability of the bacteria as well as negative controls in the form of sterility controls and incubations without test compound. As positive controls N-metyl-N-nitro-N-nitrosoguanidine and benzo(a)pyrene-4,5-oxide were used in the experiments for directmutagenicity and 3-methylcholanthrene, benzo(a)pyrene and 2-aminoanthracen
in the standard experiments with an activating system.
Results and discussion
Test results
- Metabolic activation:
- with and without
- Genotoxicity:
- positive
- Cytotoxicity / choice of top concentrations:
- other: 5000 µg/plate (without metabolic activation)
- Additional information on results:
- At doses >= 1580 µg, a precipitate, which was still present at the end of the experiment, was found on the plates. In the abscence of S-9 Mix, at 5000 µg practically all bacteria were killed. Under all other conditions, no significant decrease in survival occurred. Thus, adequate mutagenicity testing could be performed up to quite high doses. In the direct mutagenicity test, the test substance was mutagenic with TA 100, but not with the other strains. In the presence of S-9 Mix, the mutagenicity with TA 100 was potentiated and the compound became also mutagenic with WP2 uvrA and slightly with TA 98. The epoxide hydratase inhibitor and glutathione depletor 1,1,1 -trichloropropene oxide did not show any effect upon the mutagenicity of the test substance.
- Remarks on result:
- other: other: Salmonella tyhpimurium TA 100, TA 1537, TA 98; E.coli WP2 uvrA
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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