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EC number: 425-020-0 | CAS number: 191680-81-6 CGL 116
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1997-05-30 - 1997-11-26
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study (OECD test guideline 301 B)
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- GLP compliance:
- yes
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge (adaptation not specified)
- Details on inoculum:
- Inoculum Source: Sewage treatment plant (Jupiter, Florida)
Approximately 2 liters of secondary effluent were collected and prepared for testing. - Duration of test (contact time):
- 28 d
- Initial conc.:
- 20 other: mg carbon/L
- Based on:
- other: CO2 evolution
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST SUBSTANCE
- A stock solution of the test substance was not prepared due to the poor water solubility of the test substance.
- The test substance was initially dispersed in 300 mL test water with an ultrasonic bath and then added to the test container.
- A uniform suspension of the test substance was obtained after ultrasonic dispersion.
TEST CONDITIONS
- Composition of medium: Addition of 10 mL of a phosphate buffer solution and 1.0 mL each of ferric chloride, magnesium sulfate, and calcium chloride solutions to test water and bringing to a final volume of 1 L. All mineral salt solutions were prepared as described in OECD Guideline 301B
- Test temperature: 22 +/- 2 °C
- pH (mean values): 7.21 (day 0), 6.50 (day 28)
TEST SYSTEM
- Culturing apparatus:
- A total of seven 4 L test bottles were used. Two bottles were used for the blank control, two bottles were used for the test substance and one bottle served as the sterile control. The concentration of the test substance in these five bottles was 20 mg carbon/L.
- One bottle was used as a reference unit at a concentration of 19.9 mg carbon/L.
- The final bottle served as a toxicity control and contained 20 mg carbon/L (10 mg carbon/L test substance and 10 mg carbon/L as reference substance)
- To each bottle (except the sterile control bottle), 30 mL of inoculum, 550 mL of mineral salt, and 2000 mL of test water were added. The sterile control bottle received only the 550 mL of mineral salt solution.
- Air supply: purged with CO2-free air for 24 hours (~40 mL/minute)
- After purging the bottles with the CO2-free air for 24 hours, 420 mL of test water was added into each bottle in the blank unit. The reference bottle received 77.0 mL of reference substance stock solution and 343 mL of test water. The final concentration of reference substance in this solution was 19.9 mg carbon/L. Each bottle in the test unit received 87.8 mg of test substance and 420 mL of test water. The sterile control bottle received 87.8 mg of test substance, 350 mL test water and 2100 mL of isopropanol. The toxicity control bottle received 39 mL of reference stock solution, 43.9 mg of test substance and 381 mL of test water.
- Number of culture flasks/concentration: 2
- The content of each bottle was continuously stirred with a magnetic stirrer.
SAMPLING/MEASUREMENT
- The evolved CO2 from each bottle was trapped in a series of two gas-washing bottles containing 100 mL of 0.0125 M Ba(OH)2 on the days 1, 3, 5, 7, 10, 13, 17, 21, 22, 28.
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Abiotic sterile control: yes
- Reference control: yes (aniline)
STATISTICAL METHODS / DATA:
The percent biodegradability at each time interval was calculated for the test and reference systems from the results of cumulative CO2 production using the following algorithm: Percent Biodegradability = (cumulative O2 produced (mg) x 100 / TOC added (mg) x 3.67) - Reference substance:
- aniline
- Remarks:
- 20 mg carbon/L
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 4.37
- Sampling time:
- 28 d
- Remarks on result:
- other: 20 mg carbon test substance/L
- Details on results:
- The test item is not biodegradable when exposed to a 1% inoculum concentration of microorganisms maintained in an aerobic, aqueous
mineralized environment (<10% cumulative CO2 biodegradation rate within a 28-day test period). - Results with reference substance:
- Cumulative biodegradation of the reference substance was 68.6 % by the end of the 10-day window meeting the test criteria for
acceptability. - Validity criteria fulfilled:
- yes
- Executive summary:
Ready biodegradability in water was studied according to OECD guideline 301B using activated sludge as inoculum. At a concentration of 20 mg carbon/L, cumulative biodegradation only reached 4.37% by day 28. The test item (as described in section 1.2) was dispersed in the test media without vehicle. No inhibitory effect on the bacteria was observed. The reference substance demonstrated the appropriate response. Based on the results the test item is not readily biodegradable.
Reference
Description of key information
The test substance is not readily biodegradable.
Key value for chemical safety assessment
- Biodegradation in water:
- not biodegradable
- Type of water:
- freshwater
Additional information
Ready biodegradability in water was studied according to OECD guideline 301B using activated sludge as inoculum. At a concentration of 20 mg carbon/L, cumulative biodegradation only reached 4.37% by day 28. The test substance was dispersed in the test media without vehicle. No inhibitory effect on the bacteria was observed. The reference substance demonstrated the appropriate response. Based on the results the test substance is not readily biodegradable.
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