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Diss Factsheets
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EC number: 700-093-4 | CAS number: 176969-34-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vitro / ex vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 007
- Report date:
- 2007
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- There are no official guidelines for the HET-CAM Test; however, the study was conducted according to the following publications:
- Lüpke N.P. (1985): Hen’s Egg Chorio allantoic Membrane Test for Irritation Potential. Fd. Chem. Toxic. 23, pp. 287 – 291.
- Spielmann, H. (1995): HET-CAM Test. In: Methods in Molecular Biology, 43 (eds.: O’Hare, S. and Atterwill, C. K.) pp. 199 – 204.
- Spielmann, H. et al. (1996): Results of a Validation Study in Germany on Two In Vitro Alternatives to the Draize Eye Irritation Test the HET-CAM Test and the 3T3 NRU Cytotoxicity Test. ATLA 24, pp. 741 – 858. - GLP compliance:
- yes
Test material
Constituent 1
Test animals / tissue source
- Species:
- other: hen eggs
- Strain:
- other: White Leghorn
- Details on test animals or tissues and environmental conditions:
- TEST SYSTEM HEN EGG
- Source: Charles River Deutschland GmbH, Extertal
- Age at study initiation: Fresh, fertilized hen eggs
- Weight at study initiation: 55.4 g – 56.9 g
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 37.5
- Humidity (%): 62.5
Test system
- Vehicle:
- unchanged (no vehicle)
- Controls:
- other: no control eggs
- Amount / concentration applied:
- 25 µg
- Duration of treatment / exposure:
- 3.5 min
- Observation period (in vivo):
- 3.5 min (210 sec)
- Number of animals or in vitro replicates:
- Number of eggs: 3
- Details on study design:
- REMOVAL OF TEST SUBSTANCE
- Washing (if done): 0.9% aqueous NaCl solution after 3.5 minutes
SCORING SYSTEM:
After application of the test substance the chorionallantoic membrane was observed by means of a stereomicroscope until unambiguous irritation reactions were detected or up to a maximum time period of 3.5 minutes, respectively.
The time of appearance (in seconds after application) of intravascular resp. extravascular coagulation and, if applicable, other reactions (haemorrhagia, vessel lysis) were determined.
The evaluation of the reactions was performed according to the following grading:
Grading
0 - No visible change
1 - Slight reaction
2 - Moderate reaction
3 - Severe reaction
The mean time until appearance of reaction over the eggs of a treatment group was
calculated (mean time to coagulation = mtc in seconds).
TOOL USED TO ASSESS SCORE: stereomicroscope
Results and discussion
In vitro
Resultsopen allclose all
- Irritation parameter:
- other: Coagulation
- Run / experiment:
- 1, 2 and 3; observation period 210 seconds
- Value:
- 0
- Positive controls validity:
- valid
- Remarks on result:
- other: no indication of serious eye damage
- Irritation parameter:
- other: haemorrhagia
- Run / experiment:
- 1, 2 and 3; observation period: 210 seconds
- Value:
- 0
- Positive controls validity:
- valid
- Remarks on result:
- other: no indication of serious eye damage
Any other information on results incl. tables
Mean results of the positive control substances (2 replicates)
0.1 N NaOH
Grade 2 haemorrhagia after 24 sec
Grade 2 coagulation after 45 sec
1% SDS
Grade 2 haemorrhagia after 38 sec
Grade 2 coagulation after 69 sec
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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