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EC number: 617-346-9 | CAS number: 82543-15-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Feb - Mar 1999
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 999
- Report date:
- 1999
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-C (Determination of the "Ready" Biodegradability - Carbon Dioxide Evolution Test)
- GLP compliance:
- yes
Test material
- Reference substance name:
- Reference substance 001
- Cas Number:
- 82543-15-5
- Test material form:
- solid
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): sewage treatment plant "Kläranlage Berlin Ruhleben"
- Storage conditions: room temperature - Duration of test (contact time):
- 42 d
Initial test substance concentration
- Initial conc.:
- 10 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Test temperature: 21 - 23°C
- pH: 7.4 -7.7
- pH adjusted: no
- Continuous darkness: yes
- Aeration: the test solutions were supplied with filtered CO2-free air (2.0 to 5.5 L air per hour for each test vessel)
TEST SYSTEM
- Test performed in closed vessels: yes
- Details of trap for CO2 and volatile organics if used: For the determination of the CO2 produced, three CO2 absorber bottles, filled with 100 mL 0.025 N Ba(OH)2 each, were connected in series to the exit air-pipe of each test bottle. The amount of CO2 produced was determined by titration of the remaining Ba(OH)2 with 0.05 N standardized HCI.
SAMPLING
- Sampling frequency: 3, 4, 7, 9, 14, 18, 23, 28, 30, 35, 42, 44
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes, 3 vessels
- Toxicity control: yes, 1 vessel
Reference substance
- Reference substance:
- benzoic acid, sodium salt
Results and discussion
% Degradationopen allclose all
- Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 41
- Sampling time:
- 28 d
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 48
- Sampling time:
- 42 d
BOD5 / COD results
- Results with reference substance:
- 100% after 28 d.
Any other information on results incl. tables
Biological degradation (cumulative) in percent (corrected for blank CO. production) of Endiol
Test Compound | Nominal Concentration of carbon | Day of sampling | |||||||||||
3 | 4 | 7 | 9 | 14 | 18 | 23 | 28 | 30 | 35 | 42 | 44 | ||
Endiol ( ZK 38700) | 10 mg/L | 4 | 12 | 12 | 12 | 16 | 26 | 35 | 41 | 43 | 44 | 46 | 48 |
Reference (sodium acetate) | 10 mg/L | 13 | 39 | 57 | 71 | 85 | 93 | 97 | 100 | 100 | 100 | 100 | 100 |
Toxicity control (ZK 38700 + sodium acetate) | 10 mg/L + 10 mglL | 7 | 23 | 32 | 38 | 46 | 50 | 57 | 63 | 65 | 67 | 69 | 71 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- inherently biodegradable
- Conclusions:
- In accordance with the OECD guideline, the test compound Endiol is not readily biodegradable under the conditions of the test and it was not toxic to the microbes of activated sludge. However, at the end of the test period, the test substance was partly degraded, probably indicating inherent biodegradability.
- Executive summary:
The purpose of this study was to determine the ready biodegradability of Endiol (ZK 38700), which is an intermediate of the synthesis of drospirenone. The study was conducted in agreement with the OECD test guideline no. 301 B and the EC guideline. The test substance Endiol was incubated in a concentration of 10 mg carbon/L in triplicate. Additionally, a reference substance (sodium acetate) was tested in a single set according to the same procedure, in order to verify the viability and activity of the degrading microorganisms. One further set was incubated with sodium acetate at 10 mg carbon/I (reference substance) plus Endiol at 10 mg carbon/L representing a toxicity control. Furthermore, a blank control was tested in triplicate without any test or reference substance. The biological degradation of the test and reference substances was evaluated by measurement of the carbon dioxide (CO2) produced during the test period. CO2 production was determined on days 3, 4, 7, 9, 14, 18, 23, 28, 30, 35, 42 and 44. On day 43 the solutions were acidified in order to expel all dissolved CO2, and CO2 was determined on day 30. The CO2 production was calculated as the percentage of total CO2 that the test material could theoretically have produced, based on carbon content. The blank CO2 production was subtracted for correction.
The test compound Endiol was degraded to 48% at the end of the test on day 44 (42 days of incubation). The reference compound sodium acetate was degraded to 71% on day 9 (8 days of incubation) and up to 100% on day 28. In the toxicity control, the reference compound (sodium acetate) plus the test compound Endiol was degraded to 71 % on day 44 (42 days of incubation).The test compound Endiol is not readily biodegradable under the conditions of the test and it was not toxic to the microbes of activated sludge.
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