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Diss Factsheets
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EC number: 905-559-2 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Adequate for assessment.
Data source
Reference
- Reference Type:
- publication
- Title:
- Salmonella Mutagenicity Tests: II. Results From The Testing of 270 Chemicals
- Author:
- Mortelmans K, Haworth S, Lawlor T, Speck W, Tainer B and Zeiger E
- Year:
- 1 996
- Bibliographic source:
- Environmental Mutagenesis (1986) Vol 8 (7), 1-119
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- yes
- Remarks:
- neither Salmonella TA102 or E.coli strain included (but not considered to invalidate the assay)
- GLP compliance:
- not specified
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Cyclohexane
- EC Number:
- 203-806-2
- EC Name:
- Cyclohexane
- Cas Number:
- 110-82-7
- Molecular formula:
- C6H12
- IUPAC Name:
- cyclohexane
Constituent 1
Method
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Metabolic activation system:
- Arochlor 1254-induced rat and hamster metabolic activation system
- Test concentrations with justification for top dose:
- 0.000 (solvent control), 10.000, 33.000, 100.000, 333.000, 1000.000, 3333.000, 10000.000 µg per plate
- Vehicle / solvent:
- dimethyl sulfoxide (DMSO) or distilled water (0.05 ml vehicle with 0.5 ml S-9 mix and 0.05 ml overnight culture)
Controls
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- yes
- Remarks:
- potassium chloride
- Positive controls:
- yes
- Remarks:
- sodium azide for TA1535 and TA100, 4-nitro-o-phenylenediamine for TA98, 9-aminoacridine for TA97 and TA1537, 2-aminoanthracene was used with all strains with hamster and rat liver metabolic activation systems
- Details on test system and experimental conditions:
- Cyclohexane was assayed for mutagenicity in the pre-incubation assay. Concurrent solvent and positive controls were tested with and without the metabolic activation systems. At least five dose levels of the chemicals were tested, with three plates per dose level.
METHOD OF APPLICATION: preincubation
DURATION
- Preincubation period: 20 minutes at 37°C
- Exposure duration: 48 hour at 37°C
NUMBER OF REPLICATIONS: 2
DETERMINATION OF CYTOTOXICITY
- one or more of the following phenomena: appearance of his pinpoint colonies, reduced numbers of revertant colonies per plate, or thinning or absence of the bacterial lawn - Evaluation criteria:
- The criteria used for data evaluation are summarised as follows: 1) mutagenic response: a dose-related, reproducible increase in the number of revertants over background, even if the increase was less than twofold; 2) non-mutagenic response: when no increase in the number of revertants was elicited by the chemical; 3) questionable response: when there was an absence of a clear-cut dose-related increase in revertants; when the dose-related increases in the number of revertants were not reproducible; or when the response was of insufficient magnitude to support a determination of mutagenicity.
Results and discussion
Test results
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- Only results for cyclohexane presented in summary
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative with metabolic activation
negative without metabolic activation
Cyclohexane negative with and without metabolic activation - Executive summary:
This publication reported Salmonella mutagenicity tests on 270 chemicals (including cyclohexane) tested under contract to the National Toxicology Program. Cyclohexane was tested using S. typhimurium strains TA 1535, TA 1537, TA 98 and TA 100 in the presence and absence of metabolic activation (S9). The study was run using 2 independent repeats.
There was no significant increase in the numbers of revertant colonies in any strain both with and without metabolic activation. The results indicate that cyclohexane is not mutagenic in this bacterial assay.
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