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EC number: 267-140-4 | CAS number: 67801-20-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
Description of key information
The toxicity of the test item to the freshwater algae pseudokirchneriella subcapitata has been investigated and gave a 72-Hour growth rate EC50 value of 24 mg/l. The corresponding No Observed Effect Concentration was 3.1 mg/l.
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 24 mg/L
Additional information
Pseudokirchneriella subcapitata was exposed to solutions of the test item at nominal concentrations of 0.31, 0.99, 3.1, 9.9 and 31 mg/l (three replicate flasks per concentration) for 96 hours, under constant illumination and shaking at a temperature of 24 ± 1°C. The results based on the nominal test concentrations are summarised below:
TiPoint (Hours) |
Response Variable |
EC50(mg/l) |
95% Confidence Limits (mg/l) |
No Observed Effect Concentration (NOEC) (mg/l) |
Lowest Observed Effect Concentration (LOEC) (mg/l) |
||
72 |
Growth Rate |
24 |
|
* |
|
3.1 |
9.9 |
Yield |
13 |
12 |
- |
15 |
9.9 |
31 |
|
Biomass |
13 |
12 |
- |
15 |
3.1 |
9.9 |
|
96 |
Growth Rate |
26 |
|
* |
|
3.1 |
9.9 |
Yield |
13 |
11 |
- |
14 |
3.1 |
9.9 |
|
Biomass |
13 |
11 |
- |
15 |
3.1 |
9.9 |
*It was not possible to calculate 95% confidence limits for the ErC50values as the data generated did not fit the models available for the calculation of confidence limits.
Analysis of the test preparations at 0 hours showed measured test concentrations to be near nominal with the exception of the 0.31 and 3.1 mg/l test concentrations which showed measured test item concentrations of 164% and 133% of nominal respectively. Analysis of duplicate samples which had been stored frozen confirmed these results (127% and 136% of nominal respectively). A significant decline in measured test concentration was observed at 96 hours in the range of less than the limit of quantitation (LOQ) of the analytical method employed (which was determined to be 0.017 mg/l) to 3% of nominal.
Given that the results obtained from the preliminary stability analyses and additional stability analyses conducted under test conditions indicated that the test item was stable over the test period this decline was considered to be due to adsorption to the algal cells present. Whilst the preliminary recovery analyses conducted in the presence of algal cells indicated that no significant adsorption occurred this does not preclude long term adsorption over the test period to the increasing algal biomass. Given that the decline in measured concentrations was attributable to adsorption of the test item to the algal cells present it can be considered that the algae were exposed to near nominal test item concentrations for the duration of the test. As such it was considered appropriate to calculate the results based on nominal test item concentrations alone.
A re-growth test was performed which showed the test item to be algistatic but not algicidal in effect.
The study met the acceptability criteria prescribed by the Study Plan and hence was considered valid.
The preferred observational endpoint in this study is algal growth rate inhibition because it is not dependent on the test design (ECHA guidance Chapter R.7b v1.1). Since exponential growth occurred throughout the test duration of 96 hours the ErC50 values at 72 hours (24 mg/L) and 96 hours (26 mg/L) are equally valid. The lowest effect value of 24 mg/L has been used for the chemical safety assessment.
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