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EC number: 700-292-6 | CAS number: 135043-68-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vitro / ex vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Performed to GLP standards.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 009
- Report date:
- 2009
Materials and methods
Test guideline
- Qualifier:
- no guideline available
- Principles of method if other than guideline:
- The purpose of this study is to determine the eye irritation potential of trisodium 6,6',6"-(1,3,5-triazine-2,4,6-triyltriimino)trihexanoate using the SkinEthic Reconstituted Human Corneal model after a treatment period of 10 minutes. It is thought that irritant chemicals are able to penetrate the stratum corneum and are sufficiently cytotoxic to cause cell death in the underlying cell layers. Cytotoxicity is determined by the reduction of MTT to formazan by viable cells in the trisodium 6,6',6"-(1,3,5-triazine-2,4,6-triyltriimino)trihexanoate treated tissues relative to the negative control.
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- trisodium 6,6',6"-(1,3,5-triazine-2,4,6-triyltriimino)trihexanoate
- IUPAC Name:
- trisodium 6,6',6"-(1,3,5-triazine-2,4,6-triyltriimino)trihexanoate
Constituent 1
Test animals / tissue source
- Species:
- other: in vitro test
- Strain:
- other: in vitro test
- Details on test animals or tissues and environmental conditions:
- Incubation conditions: 37 C, 5% CO2 in air.
Test system
- Vehicle:
- unchanged (no vehicle)
- Controls:
- other: not applicable as in vitro test.
- Amount / concentration applied:
- Main test: 30 mg of trisodium 6,6',6"-(1,3,5-triazine-2,4,6-triyltriimino)trihexanoate
Negative control: 30 microlitres os solution A (Na2HPO4 0.142 g/l; Glucose 1.802 g/l; HEPES 7.149 g/l; KCl 0.224 g/l; NaCl 7.597 g/l)
Positive control: 30 microlitres of 1% w/v Sodium Dodecyl Sulphate (SDS) - Duration of treatment / exposure:
- 10 minutes
- Number of animals or in vitro replicates:
- No animals involved - in vitro test.
- Details on study design:
- Experimental design of this study consists of a test for direct reduction of MTT by the test materials followed by the main test. For the main test, triplicate SkinEthic tissues are treated with 30 mg of trisodium 6,6',6"-(1,3,5-triazine-2,4,6-triyltriimino)trihexanoate for 10 minutes. Triplicate tissues treated with 30 microlitres of solution A served as the negative control and triplicate tissues treated with 30 microlitres of 1% w/v Sodium Dodecyl Sulphate served as the positive control. After the exposure period, each SkinEthic tissue was rinsed and taken for MTT loading and extraction. The absorbency of triplicate alquots of the extracted MTT solution for each SkinEthic tissue is measured and the cell viability for each is calculated.
Results and discussion
In vivo
Results
- Irritation parameter:
- other: % Viability
- Remarks on result:
- other: in vitro test: see remarks on results section below for scores
- Irritant / corrosive response data:
- The mean OD50 values and mean viabilities for each treatment group are given in table 1.
Any other information on results incl. tables
Table 1. Assessment of eye irritation potential - viability of RHC tissues.
Material | Mean Tissue Viability | Mean OD540 | % Viability |
Negative Control | 0.919 | 0.937 | 100 |
0.954 | |||
Positive Control | 0.282 | 0.295 | 31.5 |
0.308 | |||
trisodium 6,6',6"-(1,3,5-triazine-2,4,6-triyltriimino)trihexanoate | 0.82 | 0.839 | 89.5 |
0.858 |
Applicant's summary and conclusion
- Interpretation of results:
- not irritating
- Remarks:
- Migrated information Criteria used for interpretation of results: expert judgment
- Conclusions:
- This study suggests that trisodium 6,6',6"-(1,3,5-triazine-2,4,6-triyltriimino)trihexanoate is considered to be a non-irritant.
- Executive summary:
In a primary eye irritation study 30 mg of trisodium 6,6',6"-(1,3,5-triazine-2,4,6-triyltriimino)trihexanoate without a vehicle was instilled onto a reconstituted human corneal epithelium model. for 10 minutes. After the exposure, the tissues were rinsed and taken for MTT testing. Irritation was scored by the method of % cell viability.
In this study, trisodium 6,6',6"-(1,3,5-triazine-2,4,6-triyltriimino)trihexanoate is not an eye irritant.
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