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EC number: 700-742-1 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2002-07-30 to 2002-09-10
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 002
- Report date:
- 2003
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.13/14 (Mutagenicity - Reverse Mutation Test Using Bacteria)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- JAPAN: Guidelines for Screening Mutagenicity Testing Of Chemicals
- Deviations:
- not specified
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- 2003-02-04
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Reaction mass of bis[2-(2-{[(prop-2-en-1-yloxy)carbonyl]oxy}ethoxy)ethyl] carbonate and diallyl 2,2'-oxydiethyl dicarbonate
- EC Number:
- 700-483-4
- Molecular formula:
- not applicable
- IUPAC Name:
- Reaction mass of bis[2-(2-{[(prop-2-en-1-yloxy)carbonyl]oxy}ethoxy)ethyl] carbonate and diallyl 2,2'-oxydiethyl dicarbonate
- Details on test material:
- - Name of test material (as cited in study report): Carbonic acid, di(2-propenyl) ester, reaction products with 2,2'-oxybis-ethanol and 2,2-bis(hydroxymethyl)-l,3-propanediol.
CAS Nr: 145272282
- Molecular formula (if other than submission substance): C7H10O3
- Molecular weight (if other than submission substance): ~142
- Substance type: organic
- Physical state: colorless transparency liquid
- Stability under test conditions: stable at room temperature
- stability in solvent: stable in water, dimethylsulfoxide and acetone
- Storage condition of test material: at room temperature, shield from light
Constituent 1
Method
- Target gene:
- Gens of Histidine Operons hisG46, hisC3076, hisC3052 and hisD6610 in Salmonella Typhimurium and gene of Tryptophan Operon (trpE) in E.coli.
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Details on mammalian cell type (if applicable):
- not applicable
- Additional strain / cell type characteristics:
- DNA polymerase A deficient
- Remarks:
- rfa mutated, resulted in removal of the lipopolysaccharide coat down to the ketodeoxyoctanoate core, presumably making the organisms more permeable to large chemical agents.
- Species / strain / cell type:
- E. coli WP2 uvr A
- Details on mammalian cell type (if applicable):
- not applicable
- Additional strain / cell type characteristics:
- DNA polymerase A deficient
- Remarks:
- carries the pKM101 plasmid to enhance error-prone repair
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9
- Test concentrations with justification for top dose:
- The maximum dose of the preliminary reverse mutation test was set at 5000 µg/plate and total six different dose levels with factor of 4 from the maximum dose (1250, 313, 78, 20 and 5 µg/plate). The maximum doses of the main reverse mutation test using Escherichia coli WP2 uvrA in the presence of metabolic activation was set at 5000 µg/plate for Escherichia coli WP2 uvrA and 1250 µg/plate for Salmonella typhimurium TA98, TA100, TA1535 and TA1537 and total six different dose levels with factor of 2 from the maximum dose. The maximum dose of the confirmatory reverse mutation test using Escherichia coli WP2 uvrA was set at 5000 µg/plate, for Salmonella typhimurium TA1535 at 313 µg/plate, and Salmonella typhimurium TA98, TA100 and TA1537 at 156 µg/plate and total six different dose levels with factor of 2 from the maximum dose (see Table 2 in "Remarks on results including tables and figures").
Controls
- Untreated negative controls:
- yes
- Remarks:
- solvent control
- Negative solvent / vehicle controls:
- yes
- Remarks:
- DMSO
- True negative controls:
- not specified
- Positive controls:
- yes
- Positive control substance:
- benzo(a)pyrene
- Remarks:
- Lot.Nr ELQ2013, ELJ6826, 106F06681, ELE2329 and SEL1402 respectively Migrated to IUCLID6: furhter positive controls: 2-aminoanthracene, 9-aminoacridine, sodium azide, furylfuramide
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Remarks:
- the number of revertant colonies was comparable with the negative (vehicle) control
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- at 625 µg/plate or more in the presence of metabolic activation
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Species / strain:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Genotoxicity:
- positive
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- at 5000 µg/plate in the presence of metabolic activation
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
The test substance increased the number of the revertant colonies twofold or more compared with the negative control with reproducibility in Escherichia coli WP2 uvrA in the presence of metabolic activation (Table 1).
Table 1 |
Reverse mutation test using Salmonella typhimurium and Escherichia coli in the presence of metabolic activation |
||||||||||
|
|
Exp. No. 11304 |
|||||||||
Number of revertant colonies per plate (mean) |
|||||||||||
Compound |
Dose (µg/plate) _ |
Base pair substitution type |
Frameshift type |
||||||||
TA100 |
TA1535 |
VP2 uvrA |
TA98 |
TA1537 |
|||||||
DMSO |
|
127 |
|
7 |
|
27 |
|
27 |
|
8 |
|
|
128 |
( 128) |
12 |
( 10) |
35 |
( 31) |
34 |
( 31) |
7 |
( 8) |
|
Test item |
|
122 |
|
7 |
|
|
|
31 |
|
6 |
|
39 |
122 |
( 122) |
10 |
( 9) |
|
|
26 |
( 29) |
4 |
( 5) |
|
|
129 |
|
8 |
|
|
|
29 |
|
8 |
|
|
78 |
122 |
( 126) |
7 |
( 8) |
|
|
30 |
( 30) |
8 |
( 8) |
|
|
99 |
|
7 |
|
43 |
|
27 |
|
6 |
|
|
156 |
100 |
( 100) |
6 |
( 7) |
45 |
( 44) |
23 |
( 25) |
4 |
( 5) |
|
|
96 |
|
4 |
|
50 |
|
24 |
|
4 |
|
|
313 |
83 |
( 90) |
6 |
( 5) |
61 |
( 56) |
25 |
( 25) |
4 |
( 4) |
|
|
0 * |
|
0 * |
|
86 |
|
0 * |
|
0 * |
|
|
625 |
0 * |
( 0) |
0 * |
( 0) |
82 |
( 84) |
0 * |
( 0) |
0 * |
( 0) |
|
|
0 * |
|
0 * |
|
95 |
|
0 * |
|
0 * |
|
|
1250 |
0 * |
( 0) |
0 * |
( 0) |
88 |
( 92) |
0 * |
( 0) |
0 * |
( 0) |
|
|
|
|
|
|
39 |
|
|
|
|
|
|
2500 |
|
|
|
|
36 |
( 38) |
|
|
|
|
|
|
|
|
|
|
0 * |
|
|
|
|
|
|
5000 |
|
|
|
|
0 * |
( 0) |
|
|
|
|
|
B[a]P |
|
877 |
|
|
|
|
|
295 |
|
64 |
|
5.0 |
1044 |
( 961) |
|
|
|
|
244 |
( 270 ) |
68 |
( 66) |
|
2AA |
|
|
|
196 |
|
|
|
|
|
|
|
2.0 |
|
|
209 |
( 203 ) |
|
|
|
|
|
|
|
|
|
|
|
|
813 |
|
|
|
|
|
|
10.0 |
|
|
|
|
771 |
( 792 ) |
|
|
|
|
|
DMSO: dimethylsulfoxide |
The test results were reproducible in the confirmatory test.
And also clear dose-related increase in the number of the revertant colonies was observed.On the other hand, the growth of the contaminant was not observed in the result of the sterility test. The comparison between the negative and positive control value, and the historical data, the negative control of Salmonella typhimurium TA100 in the presence of metabolic activation at the preliminary test was out of the range in the standard value based on the histrical data in our laboratory. But the negative and positive control value of this bacterial strain in case of absence of metabolic activation were within the range of the standard value. Therefore, it was judged that the genetic markers of and the sensitivity to mutagenic activity of this bacterial strain were appropriate. And the positive control value of this bacterial strain in case of presence of metabolic activation was within the range of the standard value. Therefore it was judged that metabolic activation worked appropriately. All of substances used as the positive controls increased the number of the revertant colonies twofold or more compared with the negative control in all bacterial strains. These results indicate that the test has been properly carried out. The environmental factor that might influence the reliability of this test was not detected. The precipitation of the test substance was not detected at any dose levels regardless of the presence or the absence of metabolic activation.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results: positive with metabolic activation in E.coli WP uvrA.
The mutagenic activity of the test substance is considered to be positive under the test conditions employed. - Executive summary:
The mutagenic activity of Carbonic acid, di(2-propenyl) ester, reaction products with 2,2'-oxybis-ethanol and 2,2-bis(hydroxymethyl)-l,3-propanediol was examined in the reverse mutation test by using bacterial strains that have two different patterns of mutation. One of the mutation is base-pair substitution of Salmonella typhimurium TA100, TA1535 and Escherichia coli WP2 uvrA, and another mutation is frameshift mutation of Salmonella typhuimurium TA98 and TA1537. The reverse mutation test was composed of the preliminary, main and confirmatory test in accordance with the test guideline applied, and the reproducibility of these test results was confirmed. The test was performed in pre-incubation methods in all bacterial strains in the presence and absence of metabolic activation. In this test, no statistical analysis was used to evaluate the test results. In the presence and absence of metabolic activation, the number of revertant colonies in each bacterial strain at all dose levels was compared with the negative control. The test substance was judged positive for the mutagenic activity when clear dose-related increase in the number of the revertant colonies and twofold or more increase in the number of the revertant colonies compared with the negative control was observed with reproducibility.
The results of the test are as follows :
1. The test substance increased the number of the revertant colonies twofold or more compared with the negative control in Escherichia coli WP2 uvrA in the presence of metabolic activation. Also, the dose-relativity was observed. And reproducibility of the test results was recognized.
2. The values of the negative controls and positive controls were appropriate in comparison with the historical data of our laboratory. Furthermore, all of the positive controls, such as 2-(2-furyl)-3-(5-nitro-2-furyl)acrylamide, sodium azide, 9-aminoacridine, benzo[a]pyrene and 2-aminoanthracene, increased the number of the revertant colonies twofold or more compared with the negative control in all bacterial strains, respectively. These results indicate that the test has been properly carried out.
3. From the foregoing results, it is concluded that the mutagenic activity of the test substance is judged positive under the test conditions employed.
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