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EC number: 212-757-6 | CAS number: 867-13-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Long-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From 2016-05-31 to 2017-03-13
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Remarks:
- Study conducted in compliance with international standard guidelines under GLP conditions. The study report was well documented with all mandatory information included.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 211 (Daphnia magna Reproduction Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- Single samples for analysis were taken from all test concentrations and the control.
Sampling: Frequency At the beginning and at the end of three intervals of 48 hours (nominal days 2 and 4, 8 and 10, 14 and 16).
Volume 3.0 mL
Storage Samples were stored in a freezer (≤15°C) until analysis.
At the end of the refreshment period, the replicates were pooled at each concentration before sampling.
Additionally, reserve samples of 3.0 mL were taken from all test solutions for possible analysis.
If not already used, these samples were stored in a freezer (≤15°C) for a maximum of three months after delivery of the draft report, pending on the decision of the sponsor for additional analysis. - Vehicle:
- yes
- Remarks:
- medium
- Details on test solutions:
- The batch of TRIETHYL PHOSPHONOACETATE tested was a colourless liquid with a purity of 99.3% (area) and the item was completely soluble in test medium at the concentrations tested. No correction was made for the purity/composition of the test substance.
Preparation of test solutions started with a stock solution of 100 mg/L (range-finding test) or the highest test concentration of 4.6 mg/L (final test). No special treatment other than a magnetic stirring period of approximately 15 minutes was needed to completely dissolve the test item in test medium. The lower test concentrations were prepared by subsequent dilutions of the stock or highest test concentration in test medium. All final test solutions were clear and colourless. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna (Crustacea, Cladocera) (Straus, 1820), at least third generation, obtained by a cyclical parthenogenesis under specified breeding conditions.
- Source: In-house laboratory culture with a known history.
- Age of parental stock (mean and range, SD): To initiate the test, young daphnids < 24 hours old were selected, from parental daphnids greater than two weeks old.
- Feeding during test: Daily, a suspension of fresh water algae - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 21 d
- Hardness:
- from 196 to 214 mg/L expressed as CaCO3
- Test temperature:
- 19.5 to 20.8°C
- pH:
- 7.6 to 8.3
- Dissolved oxygen:
- 7.8 mg/L to 10.1 mg/L
- Salinity:
- not applicable, fresh water
- Nominal and measured concentrations:
- nominal: 0.22, 0.46, 1.0, 2.2 and 4.6 mg/L
Measured: 0.16, 0.38, 0.93, 1.7 and 3.6 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: Volume: 60 ml (6 x Ø 3.5 cm), all-glass covered with a Perspex plate.
- Aeration: No
- Renewal rate of test solution (frequency/flow rate):
- No. of organisms per vessel: 1
- No. of vessels per concentration (replicates): 10
- No. of vessels per vehicle control (replicates): 20
- Biomass loading rate: Twice daily an amount of 0.25 ml of a Chlorella sp. suspension . On weekend days an amount of 0.50 ml was added in one single feed. This daily ration corresponded to 0.2 mg C/Daphnia/day, which is the recommended value for daily feeding per daphnid in the reproduction test according to the OECD Guideline 211.
TEST MEDIUM / WATER PARAMETERS
M7 medium
OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: 16h photoperiod daily
- Light intensity: intensity at the start: 608-831 lux, intensity at the end: 679-782 lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Reproduction, mortality & growth
TEST CONCENTRATIONS
- Range finding study: yes
- Test concentrations: 0.10, 1.0 and 10 mg/L
- Results used to determine the conditions for the definitive study: No significant mortality was observed in the control and the two lowest test concentrations, while all daphnids exposed to the highest test concentration of 10 mg/L died within 3 days of exposure. The first recordings of the presence of eggs in the brood pouch and the first offspring were similar to the control in the two lowest test concentrations. The total numbers of offspring produced were also comparable between the control and the two lowest test groups. - Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 3.6 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 3.6 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 3.6 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth
- Duration:
- 21 d
- Dose descriptor:
- EC10
- Effect conc.:
- > 3.6 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Duration:
- 21 d
- Dose descriptor:
- EC10
- Effect conc.:
- > 3.6 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Duration:
- 21 d
- Dose descriptor:
- EC10
- Effect conc.:
- > 3.6 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under the conditions of the present study TRIETHYL PHOSPHONOACETATE did not affect survival, growth or reproduction of Daphnia magna at an average measured concentration of 3.6 mg/L after 21 days of exposure (NOEC).
- Executive summary:
This study was designed to determine the chronic toxicity of triethylphosphonoacetate to Daphnia magna, according to the OECD 211 guideline and under GLP conditions. Exposure was conducted under semi-static conditions for 21 days.
Parental daphnids were exposed to triethylphosphonoacetate concentrations of 0.16, 0.38, 0.93, 1.7 and 3.6 mg/L and to a control treatment. The number of living adults and offsprings were counted daily. At the end of the test period, a maximum of 10% adults died. The reproduction rates are not significantly different from that of the blank control. The 21-day NOECs on Daphnia magna were equal to 3.6 mg/L based on survival and to 3.6 mg/L based on reproduction; showing that triethylphosphonoacetate is not harmful to aquatic invertebrates with long-lasting effects.
Reference
Concentrations of the test item in test medium - final test
Time of sampling |
Date of sampling |
Date of |
Concentration |
Relative to nominal |
Relative to initial |
|
Nominal |
Analysed |
|||||
2 (fresh) |
01-Sep-2016 |
13-Sep-2016 |
0 |
n.d.2 |
n.a. |
|
0.22 |
0.182 |
83 |
||||
0.46 |
0.374 |
81 |
||||
1.0 |
0.910 |
91 |
||||
2.2 |
1.85 |
84 |
||||
4.6 |
4.05 |
88 |
||||
4 (old) |
03-Sep-2016 |
13-Sep-2016 |
0 |
n.d. 2 |
n.a. |
n.a. |
0.22 |
0.156 |
71 |
85 |
|||
0.46 |
0.347 |
76 |
93 |
|||
1.0 |
0.875 |
87 |
96 |
|||
2.2 |
1.80 |
82 |
97 |
|||
4.6 |
3.63 |
79 |
89 |
|||
8 (fresh) |
07-Sep-2016 |
13-Sep-2016 |
0 |
0.0183 |
n.a. |
|
0.22 |
0.177 |
81 |
||||
0.46 |
0.369 |
80 |
||||
1.0 |
0.960 |
96 |
||||
2.2 |
1.89 |
86 |
||||
4.6 |
4.01 |
87 |
||||
10 (old) |
09-Sep-2016 |
13-Sep-2016 |
0 |
0.0183 |
n.a. |
n.a. |
0.22 |
0.172 |
78 |
97 |
|||
0.46 |
0.381 |
83 |
103 |
|||
1.0 |
0.795 |
79 |
83 |
|||
2.2 |
1.73 |
79 |
92 |
|||
4.6 |
3.73 |
81 |
93 |
|||
14 (fresh) |
13-Sep-2016 |
26-Sep-2016 |
0 |
n.d. |
n.a. |
|
0.22 |
0.134 |
61 |
||||
0.46 |
0.362 |
79 |
||||
1.0 |
0.990 |
99 |
||||
2.2 |
1.58 |
72 |
||||
4.6 |
3.10 |
67 |
||||
16 (old) |
15-Sep-2016 |
26-Sep-2016 |
0 |
n.d. |
n.a. |
n.a. |
0.22 |
0.128 |
58 |
96 |
|||
0.46 |
0.439 |
95 |
121 |
|||
1.0 |
1.04 |
104 |
105 |
|||
2.2 |
1.46 |
66 |
92 |
|||
4.6 |
3.19 |
69 |
103 |
1 Samples were stored in the freezer (≤ -15°C) until the day of analysis.
2 Result of re-analysis, because first results were not reliable due to carry-over.
3 Estimated value, calculated by extrapolation of the calibration curve. This is probably caused by carry over since a similar response was observed in the analytical blank.
n.d.Not detected.
n.a.Not applicable.
Mortality (immobility) of parental daphnids at the end of the test
TRIETHYL PHOSPHONOACETATE Average conc. (mg/L) |
Introduced |
Mobile |
Immobile |
% Immobility |
Control |
20 |
18 |
2 |
10 |
0.16 |
10 |
10 |
0 |
0 |
0.38 |
10 |
10 |
0 |
0 |
0.93 |
10 |
9 |
1 |
10 |
1.7 |
10 |
9 |
1 |
10 |
3.6 |
10 |
9 |
1 |
10 |
Age at first reproduction (day)
TRIETHYL PHOSPHONOACETATE Average conc. (mg/L) |
Mean |
Median |
Min |
Max |
n |
Control |
9.2 |
9.5 |
8.5 |
12.5 |
19 |
0.16 |
8.8 |
8.5 |
8.5 |
9.5 |
10 |
0.38 |
8.8 |
8.5 |
8.5 |
10.5 |
10 |
0.93 |
8.9 |
8.5 |
8.5 |
10.5 |
10 |
1.7 |
8.7 |
8.5 |
8.5 |
10.5 |
10 |
3.6 |
8.7 |
8.5 |
7.5 |
9.5 |
9 |
Min –minimum value, Max – maximum value
Group mean cumulative number of juveniles per surviving parent and reduction of reproduction at the end of the test
TRIETHYL PHOSPHONOACETATE Average conc. (mg/L) |
Mean |
Std. Dev. |
n |
%Reduction |
Control |
135.3 |
15.07 |
18 |
|
0.16 |
134.0 |
25.67 |
10 |
0.9 |
0.38 |
134.4 |
14.92 |
10 |
0.6 |
0.93 |
133.3 |
12.48 |
9 |
1.4 |
1.7 |
129.9 |
15.18 |
9 |
4.0 |
3.6 |
124.9 |
13.32 |
9 |
7.7 |
Incidence of immobilised offspring and aborted eggs
Abnormality |
TRIETHYL PHOSPHONOACETATE, Average conc. (mg/L) |
|||||
Control |
0.16 |
0.38 |
0.93 |
1.7 |
3.6 |
|
Immobile offspring |
3 |
2 |
2 |
2 |
2 |
3 |
Aborted eggs |
50 |
30 |
44 |
30 |
31 |
8 |
n.o. – not observed
Group mean body lengths (mm) and reduction of growth of parental daphnids at the end of the test.
TRIETHYL PHOSPHONOACETATEAverage conc. (mg/L) |
Mean (mm) |
Std. Dev. |
n |
%Reduction |
Control |
4.83 |
0.481 |
18 |
|
0.16 |
5.10 |
0.169 |
10 |
-5.5 |
0.38 |
5.20 |
0.226 |
10 |
-7.6 |
0.93 |
5.32 |
0.107 |
9 |
-10.2 |
1.7 |
5.21 |
0.176 |
9 |
-7.8 |
3.6 |
5.07 |
0.286 |
9 |
-4.9 |
Description of key information
The 21-day chronic toxicity of TEPA to Daphnia magna was studied under semi-static conditions according to OECD guideline 211. Daphnids were exposed to control and test chemical at measured concentration of 0.16, 0.38, 0.93, 1.7 and 3.6 mg/L for 21 days.
The 21-day NOEC for reproduction was 3.6 mg/L (the highest dose tested).
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Dose descriptor:
- NOEC
- Effect concentration:
- 3.6 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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