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EC number: 203-828-2 | CAS number: 111-05-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to soil microorganisms
Administrative data
- Endpoint:
- toxicity to soil microorganisms
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From November 03, 2016 to December 06, 2016.
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 016
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 216 (Soil Microorganisms: Nitrogen Transformation Test)
- Deviations:
- yes
- Remarks:
- Two deviations were observed during this study. The deviations are not critical and have no influence on the results of this study.
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- N-(2-hydroxypropyl) Oleamide
- Cas Number:
- 111-05-7
- IUPAC Name:
- N-(2-hydroxypropyl) Oleamide
- Test material form:
- solid
- Remarks:
- Beige waxy solid
Constituent 1
Sampling and analysis
- Analytical monitoring:
- not required
Test substrate
- Vehicle:
- yes
- Details on preparation and application of test substrate:
- The test item “N-(2-HYDROXYPROPYL) OLEAMIDE” is dispersible in water and the deionized water was used as the carrier. The test item was directly added in the testing solutions and mixed with the soil in order to improve the homogeneity and also to increase the bioavailability of the test item in soil for the microorganisms.
Test organisms
- Test organisms (inoculum):
- soil
Study design
- Total exposure duration:
- 28 d
Test conditions
- Test temperature:
- 20+/- 2°C
- Moisture:
- The moisture content of the samples was checked and adjusted two or three times a week in order to maintain the moisture content of soil within 40 - 60 ± 5 % of Water Holding Capacity (WHC).
In order to respect the recommendation, the moisture content of soil samples was calculated for the lower and the highest WHC interval:
0.3482*0.40*100 = 13.9% for 40% WHC
0.3482*0.60*100 = 20.9 % for 60% WHC
During the period of incubation (28 days), the moisture contents of soil samples were well within 13.9 % and 20.9% which corresponds to 40 to 60% WHC and were compliant to the guideline OECD 216. - Nominal and measured concentrations:
- As the test item was non agrochemicals, five concentrations were tested according the preliminary test, non GLP and in agreement with the sponsor, the nominal concentrations were as follow: 500 – 1050 – 2200 – 4630 – 9720 mg test item / kg dry soil.
- Reference substance (positive control):
- no
Results and discussion
Effect concentrations
- Key result
- Duration:
- 28 d
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 9 720 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- nitrate formation rate
- Details on results:
- On day 0, nitrate contents were similar to the Control group and for the treated groups. The soil LUFA 2.3 contains nitrate and the repartition between the Control group and for the treated groups was homogeneous.
After 14 days of incubation period, the formed nitrate content increased in the control group and in the first two treated groups and two phenomena were observed:
- A strong stimulation was observed on both nominal concentration of 500 and 1050 mg/Kg soil dry weight in comparison to the control,
- A strong inhibition was recorded on both concentrations 4630 and 9720 mg/Kg soil dw and the inhibition rates were respectively - 59.9 % and - 87.1%
After 28 days of incubation, the micro-organisms responsible of the formation of nitrate were not affected at any tested concentration. A strong stimulation was observed in all tested concentrations with formed nitrate contents very high in comparison to the control. - Reported statistics and error estimates:
- The formed NO3-N in each treatment was compared with NO3-N in the control. A dose-response curve versus time was prepared. Statistical evaluation of the test results was performed with an ANOVA analysis at 5% significant level (Minitab 17, statistical software) and a NOEC was determined but no LOEC. The results did not permit to calculate a regression in order to determine an estimation of the ECx values
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- All the validity criteria were successful.
No adverse effects of the test item “N-(2-HYDROXYPROPYL) OLEAMIDE” on nitrogen transformation in soil was observed at any tested concentration (500, 1050, 2000, 4630 and 9720 mg/Kg soil dry weight) after 28 days of incubation. A NOEC value was determined as follows: NOEC = 9720 mg/Kg soil dry weight. - Executive summary:
The aim of this study was to investigate long term potential effects after a single exposure of the test item “ N-(2-HYDROXYPROPYL) OLEAMIDE” on Nitrogen transformation activity of soils microorganisms according to the OECD 216 guideline (January 21, 2000).
Sieved soil was amended with powdered Lucerne meal and either treated with the test item or left untreated (Control). As the test item was non agrochemicals, five concentrations were tested according the preliminary test, non GLP and in agreement with the sponsor, the concentrations were as follow: 500 – 1050 – 2200 – 4630 – 9720 mg test item / kg dry soil.
The untreated (control) and treated (500 – 1050 – 2200 – 4630 – 9720 mg/Kg soil dry weight) samples were incubated in the dark at 20+/- 2°C. The moisture content of the samples was checked and adjusted two or three times a week. NO3-N content was determined in the control and treated soils at 0, 14 and 28 days after treatment by ionic chromatography.
No significant decreases in the nitrogen transformation were found for any concentration of the test item at the end of the 28 days incubation period in comparison to the control. A NOEC value was determined as follows: NOEC = 9720 mg/Kg soil dry weight.
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