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Diss Factsheets
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EC number: 916-226-6 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Eye irritation
Administrative data
- Endpoint:
- eye irritation
- Remarks:
- other: in vitro
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: guideline study
Cross-reference
- Reason / purpose for cross-reference:
- reference to same study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 011
- Report date:
- 2011
Materials and methods
- Principles of method if other than guideline:
- In vitro study for assessing ocular irritation of compounds using a human epithelial corneal cell model. The model used is standardized and commercially available (SkinEthic™ Human Corneal Epithelial Model (HCE); SkinEthic, France).
- GLP compliance:
- yes
Test material
- Reference substance name:
- Benzyldodecylbis(2-hydroxypropyl)ammonium chloride
- EC Number:
- 265-345-3
- EC Name:
- Benzyldodecylbis(2-hydroxypropyl)ammonium chloride
- Cas Number:
- 65059-91-8
- Molecular formula:
- C25H46NO2.Cl
- IUPAC Name:
- N-benzyl-N,N-bis(2-hydroxypropyl)dodecan-1-aminium chloride
- Details on test material:
- Content of components of Katalysator WAZ 5596-8:
content of quaternary propoxylated fatty amine 54.6%
content of propoxylated fatty amine 1.3%
content of hvdrochloride propoxylated fatty amine 14.6 %
propane-1,2-diol 5.1%
a-chlorotoluene 12.9 %
sulfated ash <0.05%
water content 8.3 %
content of chloride 6.0%
Constituent 1
Test animals / tissue source
- Species:
- other: not applicable - human corneal epithelial model (HCE)
- Strain:
- other: not applicable - human corneal epithelial model (HCE)
Test system
- Vehicle:
- other: not applicable - human corneal epithelial model (HCE)
- Controls:
- other: not applicable - human corneal epithelial model (HCE)
- Duration of treatment / exposure:
- After an exposure period of 60 minutes, followed by a 16 hours post-treatment incubation period, the cell viability was measured
- Observation period (in vivo):
- not applicable - human corneal epithelial model (HCE)
- Number of animals or in vitro replicates:
- not applicable - human corneal epithelial model (HCE)
Results and discussion
In vivo
Results
- Irritation parameter:
- other: cell viability
- Basis:
- mean
- Time point:
- other: 60 minutes followed by a 16 h post-treatment period
- Score:
- 1.22
- Max. score:
- 100
- Reversibility:
- other: not applicable - human corneal epithelial model (HCE)
- Remarks on result:
- other: irritant - scores in [%] viability
Any other information on results incl. tables
A test substance is predicted to be an ocular irritant if the mean relative tissue viability (%) exposed to the test substance is < 50%.
As can be seen from the information in the Table below the test substance Katalysator WAZ 5596 B was detected as irritant to the cornea epithelium in this test model.
Compound Cell viability [%] Evaluation
Katalysator WAZ 5596 B 1.22 irritant
Positive control 8.70 irritant
Negative control 100.00 non-irritant
Applicant's summary and conclusion
- Interpretation of results:
- irritating
- Remarks:
- Migrated information
- Executive summary:
This in vitro study for assessing ocular irritation of compounds using a human epithelial corneal cell model was performed in 2011. The model used is standardized and commercially available (SkinEthic™ Human Cornea I Epithelial Model (HCE); SkinEthic,
France).
Undiluted Katalysator WAl 5596 B was applied topically to the HCE tissue, i.e. 30 µl per insert.
After an exposure period of 60 minutes, followed by a 16 hours post-treatment incubation period, the cell viability was 1.22% as measured by a MTT conversion assay.
The results of the concurrent negative (NC, PBS) and positive control (PC, 1 H-1 ,2,4-Triazole-3-thiol) demonstrated the viability (NC) and sensitivity (PC) of the test model.
The results show that Katalysator WAZ 5596 B is predicted as ocular irritant under the conditions of this test method.
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