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EC number: 479-540-8 | CAS number: 61007-89-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2002-12-13 to 2003-01-29
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: OECD study, GLP
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 003
- Report date:
- 2003
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- -
- EC Number:
- 479-540-8
- EC Name:
- -
- Cas Number:
- 61007-89-4
- Molecular formula:
- C27 H27 Mn N4 O3
- IUPAC Name:
- manganese(3+) 2-[({2-[bis(2-{[(2-oxidophenyl)methylidene]amino}ethyl)amino]ethyl}imino)methyl]benzen-1-olate
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Sampling method: Samples were taken at the beginning and the end of each renewal period and analysed for transformation products.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: As the test item is prone to hydrolysis (please refer to section 5.1.2), the first stock solution was prepared as close as practically possible to the start of the test. This treatment fulfilled the recommandations of the OECD for poorly soluble substances subject to hydrolysis (OECD, 2000). To maintain the test concentrations constant during exposure, the test was performed under semi-static conditions. The stock solutions were renewed every 24 hours. Each stock solution was prepared as follows: 400 mg test item were weighed, dissolved in 4000 mL tap water. The stock solution was homogenised by ultrasonic treatement for about 10 minutes and then stirred for about 5 minutes. Due to the poor solubility of the test item, the not dissolved parts of the test item were separated by filtration (0.45 µm). The filtration was carried out under pressure with a flow rate not greater than 100 mL/min. The filter was preconditioned with 1000 mL of the stock solution. Afterwards, the rest of the stock solution was filtrated and the filtrate taken to prepare the test concentrations. Additonally, due to the light sensitivity of the test item, the glass vessel was covered with aluminium toil.
Test organisms
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: Zebrafish {Danio rerio)
- Source: Zoo Tropic, Burgfelderstrasse 3, 4055 Basel, Switzerland
- Length at study initiation: (mean) 27 mm (range 25-29 mm) based on 7 fish of the control
- Weight at study initiation: (mean) 0.19 g (range 0.14-0.22 g) based on 7 fish of the control
- Feeding during test: Fish were not fed during the test period.
- Food type: commercially prepared zebrafish food diet: Mat Nr. 228221 DE, Tretra Werke 49304 Melle, Germany.
ACCLIMATION
- Acclimation period: at least 12 days
- Type and amount of food: commercially prepared zebrafish food diet: Mat Nr. 228221 DE, Tretra Werke 49304 Melle, Germany.
- Feeding frequency: Fish used in the test were not fed 48 hours prior to initiation of the test.
- Health during acclimation (any mortality observed):
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
Test conditions
- Hardness:
- 142 mg CaCO3/L
- Test temperature:
- 21.6 - 22.2°C
- pH:
- 7.7 and 8.0
- Dissolved oxygen:
- 95 - 98%
- Nominal and measured concentrations:
- 100 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 5 litre glass aquaria with a test volume of 3 litre
- Aeration: slightly aeration
- Renewal rate of test solution: 24 h renewal
- No. of organisms per vessel: 7 fish per concentration and control
- No. of vessels per concentration: Performed with one test chamber per concentration and control
- Biomass loading rate: 0.52 mg/L
TEST MEDIUM / WATER PARAMETERS
- in accordance to the guideline
OTHER TEST CONDITIONS
- Photoperiod: Fluorescent light, 16 hours daily with 30 min transition period - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- - Behavioural abnormalities: No sublethal effects such as changes in swimming behaviour, respiratory function, pigmentation or loss of equilibrium were observed in the control as well as in the treatment concentration of 100 mg/L during the 96-hour test period.
- Mortality of control: 1 fish
- Because the test item is prone to hydrolysis (please refer to section 5.1.2), no suitable method was avalaible to quantify the test item in water. Therefore, all biological results are related to the nominal concentrations of the test item. - Reported statistics and error estimates:
- Due to the absence of mortality during the exposure, the median lethal concentration (LC50) was estimated without a statistical program.
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.