Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 700-776-7 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study according to OECD 202
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 013
- Report date:
- 2013
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Reaction mass of mono-[(1,3-dihydro-1,3-dioxo-2H-isoindol-2-yl)methyl]-5,12-dihydroquino[2,3-b]acridine-7,14-dione and di-[(1,3-dihydro-1,3-dioxo-2H-isoindol-2-yl)methyl]-5,12-dihydroquino[2,3-b]acridine-7,14-dione and 5,12-dihydroquino[2,3-b]acridine-7,14-dione
- EC Number:
- 700-776-7
- Molecular formula:
- C29H17O4N3 (unspecified isomers) / C38H22O6N4 (unspecified isomers) / C20H12O2N2
- IUPAC Name:
- Reaction mass of mono-[(1,3-dihydro-1,3-dioxo-2H-isoindol-2-yl)methyl]-5,12-dihydroquino[2,3-b]acridine-7,14-dione and di-[(1,3-dihydro-1,3-dioxo-2H-isoindol-2-yl)methyl]-5,12-dihydroquino[2,3-b]acridine-7,14-dione and 5,12-dihydroquino[2,3-b]acridine-7,14-dione
- Details on test material:
- - Physical state: Red powder
- Storage condition of test material: Room temperature, protected from light, in original container.
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
The water accommodated fractions (WAF) was prepared with a nominal loading of the test item of 100 mg/L as specified below.
100 mg/L of the test item were weighed out and transferred with an appropriate amount of dilution water into a brown glass flask. This dispersion was shaken for 24 h with 20 rpm at room temperature.
After a separation phase of 1 h, the aqueous phase or WAF was removed by siphoning from the approximate centre of the glass
flask to give the 100 mg/L loading rate WAF to be used as a stock WAF.
The Iimit loading Ievei of 100 mg/L was based on the results of a non GLP preliminary range finding test conducted at the test facility und er static conditions.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Strain: Daphnia magna STRAUS (Clone 5).
- Source: Institut für Wasser-, Boden- und Lufthygiene (WaBolu).
- Age at study initiation (mean and range, SD): 2 to 24 h old daphnids were used for the study. They will be obtained by removing the parental daphnids twice within 22 h.
- Culture: ln glass vessels (2 - 3 L capacity) with approximately 1.8 L culture medium, at 20 ± 2°C, in an incubator, 16 h illumination, light intensity of max. 20 ln glass vessels (2 - 3 L capacity) with approximately 1.8 L culture medium, at 20 ± 2°C, in an incubator, 16 h illumination, light intensity of max. 20 µE*m^-2*s^-1. The pH should be in the range of 6 to 9.
- Culture medium: Elendt M4, according to ELENDT (1990), modified to a total hardness of 160 to 180 mg CaCO3/L.
Culture feeding: At least 5 times per week ad libitum with a mix of unicellular green algae, e.g. Pseudokirchneriella subcapitata, Desmodesmus
subspicatus and Chlorella vulgaris, with an algae cell density of > 10^6 cells/m L.
- Feeding during test: None
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- 160 to 180 mg CaCO3/L.
- Test temperature:
- 18 - 22°C, ± 1 °C constant
- pH:
- 7.88 - 8.08
- Dissolved oxygen:
- 7.55-8.67 mg/L
- Nominal and measured concentrations:
- 100 mg/L (nominal, WAF)
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass beakers (4 cm ID x 7 cm H), 50 ml capacity.
- Aeration: No
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 1
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Same as for culture.
OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: 16/8 h Iight/dark cycle.
- Light intensity: Diffuse light, light intensity of max. 20 Diffuse light, light intensity of max. 20 µE*m^-2*s^-1
- Prior to the start of the exposure (0 h), the water quality parameters (i.e. pH value, dissolved oxygen concentration, temperature, conductivity and total hardness) of the dilution water were measured. ln the fresh media (0 h), the water quality parameters (i.e. pH values, dissolved oxygen concentrations) were measured in one additional replicate of the WAF and the control. ln the old media (48 h), the water quality parameters were measured in all replicates of the WAF and the control. The room temperature was recorded throughout the test period.
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Immobilisation was determined in all groups after 24 and 48 h. An animal is considered to be immobile, if it is not able to swim within 15 sec after gentle agitation of the test vessel.
TEST CONCENTRATIONS
- Justification for using less concentrations than requested by guideline: The Iimit loading Ievei of 100 mg/L was based on the results of a non GLP preliminary range finding test conducted at the test facility under static conditions.
- Range finding study: Yes
- Test concentrations: 100 mg/L WAF (No analytical evaluation of the Iimit concentration and the control was carried out. The solubility of the test item in water turned out to be lower than the Iimit of quantification of the analytical system of 0.2 mg test item/L.) - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 48 h
- Dose descriptor:
- EL50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: loading rate (WAF)
- Remarks on result:
- other: No acute toxic effects within the range of solubility.
- Duration:
- 48 h
- Dose descriptor:
- EL10
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: loading rate (WAF)
- Remarks on result:
- other: No acute toxic effects within the range of solubility.
- Duration:
- 24 h
- Dose descriptor:
- EL50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: loading rate (WAF)
- Remarks on result:
- other: No acute toxic effects within the range of solubility.
- Duration:
- 24 h
- Dose descriptor:
- EL10
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: loading rate (WAF)
- Remarks on result:
- other: No acute toxic effects within the range of solubility.
- Results with reference substance (positive control):
- The percentage immobility for the reference item was determined after 24 h. The EC50-value with 95 % confidence interval (Cl) was calculated by sigmoidal dose-response regression. The EC50-value for the most recent of the monthly performed reference tests was:
EC50 : 1.92 mg/L (CI 1.74- 2.10 mg/L)
The EC50-value of the reference item potassium dichromate after 24 h is within the prescribed concentration range of 1.0 - 2.5 mg/L of quality criteria according to AQS P 9/2 (05/1996) for daphnids clone 5 cultured in Elendt M4 medium. The EC50-value of the reference item is also within the recommended range of 0.6 - 2.1 mg/L according to OECD-Guideline 202.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- With high probability acutely not harmful to aquatic invertebrates. No acute toxic effects within the range of solubility.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.