Cashew (Anacardium occidentale) Nutshell Extract, Decarboxylated, Distilled, oligomerisation products with 1-chloro-2,3-epoxypropane

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)

Version / remarks:

17. JUL 1992

Deviations:

yes

Remarks:

not critical for the outcome of the study

GLP compliance:

yes (incl. QA statement)

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic (adaptation not specified)

Details on inoculum:

- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure):
The sludge was taken from the activation basin of the ESN (Stadtentsorgung Neustadt) sewage treatment plant, Im Altenschemel, NW-Lachen-Speyerdorf. Date of collection: 19. Oct 2012, batch no: 20121019.
- Pretreatment:
The sludge was filtrated, washed with tap water twice, then washed with and re-sus­pended in test medium. It was then aerated for >= 12 hours.
The dry matter was determined with 4700 mg suspended solids/L.

Duration of test (contact time):

28 d

Initial conc.:

ca. 25.3 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation:

CO2 evolution

Details on study design:

TEST CONDITIONS
- Composition of medium: see below
- Test temperature: 17.1 – 21.5 °C
- pH: approx. 7.4 (on day 28)
- pH adjusted: no
- Suspended solids concentration: 4700 mg suspended solids/L
- Continuous darkness: yes

TEST SYSTEM
- Culturing apparatus: 1500 mL flasks were used as test vessels, 100 mL scrubber flasks as absorbent vessels.
- Number of culture flasks/concentration: 2
- Method used to create aerobic conditions: aerated with purified (by activated charcoal), CO2-scrubbed, moistened air
- Measuring equipment: IC measurement using the carbon analyser TOC multi N/C 2100S, Analytik Jena
- Test performed in closed vessels due to significant volatility of test substance: no information
- Test performed in open system: assumed
- Details of trap for CO2 and volatile organics if used: 0.25-m-NaOH

SAMPLING
- Sampling frequency: on days 0, 2, 4, 7, 9, 11, 14, 18, 23 and 29

CONTROL AND BLANK SYSTEM
- Inoculum blank: YES
- Toxicity control: YES

Reference substance:

aniline

Parameter:

% degradation (CO2 evolution)

Value:

25.6

St. dev.:

0.64

Sampling time:

29 d

Details on results:

see below

Results with reference substance:

see below

Degradation values in %

Day	Positive Control 1	Positive Control 2	Positive Control Mean	Test 1	Test 2	Test Mean	Abiotic Control	Toxicity Control
2	2.0	1.2	1.6	0.1	-0.6	-0.2	0.9	0.2
4	19.6	4.6	12.1	-1.1	-2.2	-1.7	1.1	2.0
7	46.0	30.9	38.4	0.1	-3.2	-1.5	2.6	17.3
9	65.5	42.3	53.9	-0.8	-3.6	-2.2	2.1	24.0
11	73.3	48.1	60.7	-1.0	-2.3	-1.6	1.3	30.8
14	79.2	60.0	69.6	1.6	-2.4	-0.4	1.2	36.9
18	85.1	71.7	78.4	9.9	3.1	6.5	1.3	41.5
23	81.7	72.7	77.2	17.1	16.2	16.7	0.7	43.3
29	79.7	75.3	77.5	25.1	26.0	25.6	0.2	48.1

Validity criteria fulfilled:

yes

Interpretation of results:

inherently biodegradable

Conclusions:

The registered substance degraded to 25.6% over 28 days in a GLP-study according to OECD 301B.

Executive summary:

The test item CARDOLITE NC-513 was tested using a concentration of nominally 20 mg organic carbon/L (corresponding to 25.3 mg CARDOLITE NC-513/L) in test medium following OECD 301B and EU-Method C.4-C.

Aniline was chosen as positive control. Activated sludge was used as inoculum (concentration in the test 25.0 mg dry matter/L). The test was left running for 28 days. All validity criteria were met. Degradation of the positive control was 61 % after elevendays.

The following data were determined for the test item CARDOLITE NC-513:

10-day-window: starts at day 20
degradation at the end of 10-day-window: not detected
degradation at the end of the test: 25.6 %
pass level following guideline: 60% at the end of 10-day-window

 

Therefore, regardless of the 10-day-window, the test item CARDOLITE NC-513 is not readily biodegradable following OECD 301B/EU C.4-C.

Description of key information

- 25.6 % biodegradation over 28 days [using the CO₂ evolution test of OECD 301B; GLP];

- the substance is considered to be inherently biodegradable

Key value for chemical safety assessment

Biodegradation in water:

inherently biodegradable

Type of water:

freshwater

Additional information

The registered substance degraded to 25.6% over 28 days in a GLP-study according to OECD 301B.