Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 200-431-6 | CAS number: 59-50-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_toxicological-information.png)
Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 013
- Report date:
- 2013
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 405 (Acute Eye Irritation / Corrosion)
- GLP compliance:
- yes
Test material
- Reference substance name:
- Chlorocresol
- EC Number:
- 200-431-6
- EC Name:
- Chlorocresol
- Cas Number:
- 59-50-7
- Molecular formula:
- C7H7ClO
- IUPAC Name:
- 4-chloro-3-methylphenol
- Test material form:
- solid: crystalline
- Details on test material:
- - Name of test material (as cited in study report): chlorocresol
- Substance type: Organic
- Physical state: solid
Constituent 1
Test animals / tissue source
- Species:
- rabbit
- Strain:
- New Zealand White
- Details on test animals or tissues and environmental conditions:
- TEST ANIMALS
- Source: Institute for Industrial Research & Toxicology
- Sex: Female
- Age at study initiation: 10 to 12 weeks
- Weight at study initiation: 2.0kg ±200g
- Housing: Rabbit was housed in stainless steel cages provided with stainless steel mesh bottom and facilities for food and water bottle
- Diet (e.g. ad libitum): Pelleted feed supplied by Pranav agro Industries Ltd., B7/6 Ramesh Nagar, Delhi, India
- Water (e.g. ad libitum): Community tap water passed through ‘Aqua Guard on line water filter’ was kept in bottles, ad libitum
- Acclimation period: 24 hours
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22-25°C
- Humidity (%): 40-60%
- Air changes (per hr): 10-15 air changes per hour
- Photoperiod (hrs dark / hrs light): illumination cycle set to 12 hours artificial fluorescent light and 12 hours dark
Test system
- Vehicle:
- water
- Controls:
- yes
- Amount / concentration applied:
- 0.1 gm moistened with distilled water
- Duration of treatment / exposure:
- 24 hours
- Observation period (in vivo):
- upto 21 days after exposure
- Number of animals or in vitro replicates:
- 3
- Details on study design:
- PREPARATION AND TREATMENT OF ANIMALS
Selection of species:
The albino rabbit is the preferable laboratory animal and only healthy young adult animals are used.
Preparation of animals:
Both eyes of each experimental animal provisionally selected and examined for any eye irritation, ocular defects and pre-existing corneal injury within 24 hours before testing of compound.
TEST PROCEDURE:
The test compound Chlorocresol (CAS No.-59-50-7) in the amount of 0.1 gm moistened with distilled water and then placed in the conjunctival sac of one eye of each animal after gentle pulling the lower eye lid away from the eye ball. The eye lids were then gently held together for about one second to prevent the loss of the material. The other eyes which remains untreated, served as control.
Eye Irrigation:
The eyes of the test animal did not washed for at least 24 hours following instillation of the test compound. At 24 hours a washout may be used if considered appropriate.
Dose level:
A dose of 0.1gm of test substance was applied into the one eye of the rabbits. The other eye, which remains untreated, serves as a control.
OBSERVATION:
The eyes were examined at 1, 24, 48 and 72 hours after test substance application. The grades of ocular reaction (conjunctiva, cornea and iris) were recorded at each observation. To determine the reversibility of the effect the animal was observed normally for 21 days. Any other lesions in the eye viz pannus, staining were observed and scored accordingly. Examination of reactions was facilitated by use of biomicroscope and hand slit lamp. After recording the observations at 24 hours, the eyes were further examined with the aid of fluorescein. Individual animal weight before and during the study was also observed
Results and discussion
In vivo
Results
- Irritation parameter:
- overall irritation score
- Basis:
- mean
- Time point:
- other: 72 hours
- Score:
- 58
- Max. score:
- 110
- Reversibility:
- fully reversible
- Remarks on result:
- other: Severely irritating
- Irritant / corrosive response data:
- Irritation Scoring
The test compound Chlorocresol (CAS No.: 59-50-7) when applied to the eye of New Zealand white rabbit at the dose level of 0.1 gm showed cornea discernible translucent area, details of iris slightly obscured, iris showed sluggish reaction to light, conjunctiva observed crimson red colour, half closed of eye lid and eye discharge moistening eye lids and hairs just adjacent to the lids.
Clinical Signs
The test compound Chlorocresol (CAS No.: 59-50-7) applied in conjunctival sac of rabbits did not show any observable clinical signs throughout the observation period of 21 days.
Any other information on results incl. tables
GRADING OF OCULAR LESIONS
S.No/ Sex |
|
OBSERVATION |
Score |
Total |
Total Score |
|||
1/F
|
1 hr |
24hrs |
48 hrs |
72 hrs |
||||
Cornea |
A. Opacity-Degree of Density |
1 |
2 |
2 |
2 |
7 |
7×12×5=420 |
|
B. Area of Cornea Involved |
3 |
3 |
3 |
3 |
12 |
|||
Iris |
A. Values |
1 |
1 |
2 |
2 |
6 |
6×5=30 |
|
Conjunctivae |
A. Redness |
3 |
3 |
3 |
3 |
12 |
12+12+11×5=175 |
|
B. Chemosis |
3 |
3 |
3 |
3 |
12 |
|||
C. Discharge |
2 |
3 |
3 |
3 |
11 |
|||
|
|
Total |
625 |
|||||
|
|
Mean |
156.3 |
At 72 hours,
Cornea score: 3x2x5= 30
Iris Score: 2x5= 10
Conjunctivae score: (3 +3 +3)x2 = 18
Total Score: 30 +10 +18= 58
Applicant's summary and conclusion
- Interpretation of results:
- Category 1 (irreversible effects on the eye)
- Remarks:
- Migrated information Criteria used for interpretation of results: EU
- Conclusions:
- The test compound Chlorocresol when applied to the eye of New Zealand white rabbit at the dose level of 0.1 gm showed cornea discernible translucent area, details of iris slightly obscured, iris showed sluggish reaction to light, conjunctiva observed crimson red colour, half closed of eye lid and eye discharge moistening eye lids and hairs just adjacent to the lids.
- Executive summary:
The test compound Chlorocresol when applied to the eye of New Zealand white rabbit at the dose level of 0.1 gm showed cornea discernible translucent area, details of iris slightly obscured, iris showed sluggish reaction to light, conjunctiva observed crimson red colour, half closed of eye lid and eye discharge moistening eye lids and hairs just adjacent to the lids.
Based on the result obtained from present study, it can be concluded that the test compound Chlorocresol is maximally irritant to the eyes of New Zealand white rabbit under test condition.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
![ECHA](/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/echa_logo.png)