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EC number: 271-178-7 | CAS number: 68516-75-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2011-12-19 to 2011-12-23
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Preparation of the saturated solution: A stock solution (1 mg/L test item was weighed out) was prepared with dilution water. Weighing of the test item for day 0 and 1 was carried out 72 h prior to test start and stored in the dark at room temperature until preparation of the saturated solution. Weighing of the test item for day 2 and 3 was carried out after 0 and 24 h of the study, respectively.The stock solution was stirred for 48 hours with a magnetic stirrer. The undissolved particles were removed by membrane filtration (membrane filter 0.45 µm, RC, MACHEREY-NAGEL). The centrifugation for 20 min at 10000 g was performed at day 0. No pellet was generated, so the procedure was declared insufficient. On the following days only membrane filtration was used.
- Eluate: Tap water of local origin was used for holding and testing. The water was filtered on activated charcoal and aerated for at least 24 h to remove possible chlorine.
- Controls: 7 fish in dilution water (without test item) were tested under the same test conditions as the test replicates. - Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: Zebrafish
- Source: All fish used in the test were gained at DR.NOACK-LABORATORIEN from a single brood stock. (supplier: Niedersächsischer Landesbetrieb für Wasserwirtschaft, Küsten- und Naturschutz, An der Scharlake 39, D-31135 Hildesheim)
- Length at study initiation: Average body length: 2.43 cm
- Weight at study initiation: Average body weight: 0.284 g
- Method of breeding: at the test facility at 23 +/- 2 °C and diffuse light (0.1 - 10 µmol photons x m-2 x s-1, natural photoperiod). The water was changed at least once per week. The dissolved oxygen concentration was more than 80 % of the air saturation value.
- Feeding during test: No feeding during test
ACCLIMATION
- Acclimation period: at least 12 days of acclimatisation and mortality < 5 % within these days before the study starts
- Acclimation conditions (same as test or not): Same as test
- Type and amount of food: 4 % of the fish body weight per feeding day. The test fish were not fed 24 h before the test started. Food: Sera Vipan; SERA GMBH, D-52518 Heinsberg
- Feeding frequency: 3 times per week.
- Health during acclimation (any mortality observed): No mortality observed, no disease treatments were administered throughout holding and testing.
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Hardness:
- Total Hardness at day 0: 55.2 mg/L
- Test temperature:
- 21.7 -23.5 °C
- pH:
- 7.64 - 7.86
- Dissolved oxygen:
- Oxygen saturation: 100%
- Nominal and measured concentrations:
- Nominal test concentrations: control and 1 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass aquaria
- Type: open, loosely covered by glass tops
- Material, size, fill volume: glass, 2 L test volume per vessel
- Aeration: Gentle aeration
- Renewal rate of test solution: daily renewal (after 0, 24, 48, 72 hours)
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: less than 1 g fish per L test solution
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Tap water of local origin was used for holding and testing. The water was filtered on activated charcoal and aerated for at least 24 h to remove possible chlorine.
- Nominal water parameters: Total hardness: 10 - 250 mg CaCO3/L
pH-value: 6.0 - 8.5
- Culture medium different from test medium: no
- Intervals of water quality measurement: pH-value, temperature and oxygen saturation were measured
measurements in all vessels at the beginning of the test and every 24 h (from old and new media). Total hardness of the water was determined at the beginning out of the test control. During the test the water temperature was recorded continuously with a data logger (once per hour).
OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: natural
- Light intensity: 0.1 - 10 µmol photons x m-2 x s-1
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Fish were considered dead if there was no visible movement (e.g. gill covers movement) and if touching of the caudal peduncle produced no reaction. Records were kept of visible abnormalities (e.g. loss of equilibrium, swimming behaviour, respiratory function, pigmentation, etc.). Observations were made after 24, 48, 72 and 96 h.
TEST CONCENTRATIONS
- Range finding study: No - Reference substance (positive control):
- no
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- >= 1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: No acute toxic effects within the range of solubility
- Key result
- Duration:
- 96 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: No acute toxic effects within the range of solubility
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- > 1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: No acute toxic effects within the range of solubility
- Details on results:
- - Behavioural abnormalities: Not observed
- Mortality of control: 0%
- Other adverse effects control: Not observed
- Abnormal responses: Not observed
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: The test item solutions were clear. - Results with reference substance (positive control):
- No reference substance tested
- Sublethal observations / clinical signs:
Observations in the Test Vessels
(n=7)
Nominal loading [mg/L]
Effect*)
Test Duration [h]
24
48
72
96
10
(1)
7/7
7/7
7/7
7/7
Control
(1)
7/7
7/7
7/7
7/7
*) The number in brackets correspond to the following observation:
(1) = Normal behaviour
Cumulative Mortality [%] in the Test Vessels
Nominal loading
[mg/L]Test Duration [h]
24
48
72
96
10
0
0
0
0
Control
0
0
0
0
- Validity criteria fulfilled:
- yes
- Conclusions:
- No acute toxic effects occurred within the range of solubility. The test substance is with high probability acutely not harmful to fish.
Reference
Description of key information
The substance is with high probability acutely not harmful to fish.
No toxic effects occured at the range of the substances solubility.
EC50 (96h) > 1 mg/L (nominal) for Brachydanio rerio (OECD 203)
Key value for chemical safety assessment
Additional information
The acute aquatic toxicity on fish was investigated in GLP guideline studies according to OECD 203 for two ‘red disazo condensation pigments’(CAS 68516-75-6 and CAS 71566-54-6) [DR.U.NOACK-LABORATORIEN, 2012; lnstitute of lndustrial Organic Chemistry, Branch Pszczyna, 2012]. Both studies used the zebrafish Danio rerio as test species. Based on the low water solubility of the test item, saturated stock solutions were prepared and the tests were conducted as limit tests with nominal concentrations of 1 mg/L (CAS 68516-75-6) and 100 mg/L (CAS 71566-54-6), respectively. After 96 hours of exposure no toxic effects on fish within the range of solubility were observed.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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