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EC number: 226-002-3 | CAS number: 5205-93-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1991-01-29 - 1991-08-01
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Remarks:
- GLP.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Version / remarks:
- EEC, Part C: Method fot the determination of Ecotoxicity, C.1: "Acute Toxicity for Fish", No. L251/146, dated Sept. 19, 1984
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Vehicle:
- no
- Test organisms (species):
- Oncorhynchus mykiss (previous name: Salmo gairdneri)
- Details on test organisms:
- TEST ORGANISM
- Common name: Rainbow trout
- Source: Forellenhof Fredersloh, D-3413 Moringen 1
- Age at study initiation (mean and range, SD):
- Length at study initiation : first experiment: 6.30 +/- 0.84 cm (Mean +/- SD); second experiment: 6.08 +/- 0.82 cm (Mean +/- SD)
- Weight at study initiation : first experiment: 2.88 +/-1.19 g (Mean +/- SD); second experiment: 3.04 +/-1.53 g (Mean +/- SD)
ACCLIMATION
- Acclimation period: For at least 14 days in the laboratories of RCC and at least for the last 7 days to the test water and temperature.
- Acclimation conditions (same as test or not): yes
- Type of food: Commercial trout diet (Brutfutter FB50, Kronen-Fischkraftfutter, D-4320 Wesel)
- Feeding frequency: During acclimation until one day before the test
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Hardness:
- 2.5 mmol/l as CaCO3
- Test temperature:
- first experiment: 15.3°C - 17.1°C
second experiment: 15.8°C - 16.8°C - pH:
- 7.6 - 9.4
- Dissolved oxygen:
- 8.3 mg/l or higher
- Nominal and measured concentrations:
- first experiment: 0, 5, 9, 15, 28, 50 mg/l (nominal)
second experiment: 0, 95, 171, 309, 555, 1000 mg/l (nominal) - Details on test conditions:
TEST SYSTEM
- Test vessel:
- Aeration: The test nedia were slightly areated during the test
- Type of flow-through (e.g. peristaltic or proportional diluter):
- No. of organisms per vessel: 10
- No. of vessels per control (replicates): 1
Test water without any additions.
CaCl2 x 2H2O : 294 mg/l
MgSO4 x 7H2O: 123 mg/l
NaHCO3: 65 mg/l
KCL: 5.8 mg/l
Water Hardness: 2.5 mmol/l as CaCO3
Alkalinity: 0.8 mmol/l
Ratio of Ca:Mg=4; Na:K=10:1
OTHER TEST CONDITIONS
- Adjustment of pH: Since in the test media of the concentrations 95-1000 mg/l, the pH values were in the range of pH 9.6-10.4, the pH values were adjusted with 1 mol HCL p.A. to pH 7.6-8.0
- Photoperiod: 16 hours per day
- Light intensity: abaout 50-300 Lux
During test duration the dissolved oxygen concentrations were in the test media always 8.3 mg/l or higher, the pH-values in the range of 7.6-9.4.
TEST CONCENTRATIONS
- Range finding study
- Test concentrations: 5, 9, 15, 28 and 50 mg/l
In the second experiment the test substance was weighed in concentrations of 95, 171, 309, 555 and 1000 mg/l and directly dissolved in each aquarium. Since the pH-values of these test media were in the range of pH 9.6 - 10.4, the pH values were adjusted with 1 mol HCl p.A. to 7.6 - 8.0 before introduction of the fish. Both experiments were performed including a control without any addition.- Reference substance (positive control):
- no
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 50 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 290 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 128-657 mg/l Confidence limit
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- 555 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 95 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- - Mortality of control: no mortality wa observed during the test in the control
- Sublethal observations / clinical signs:
The lowest lethal concentration (LLC) was 309 mg/l.
In the control no mortality was observed during the test.
No remarkable observations were made at the behaviour of the test substance in the test media.
The test substance concentrations in the analysed media were in the range of 84.9 - 114.3 % of the nominal values during test duration. The test substance was sufficiently stable under test conditions. Therefore, all repoted results are related to the nominal concentrations of the test substance.
No mortalities occured in the first experiment in the nominal concentrations 5-50 mg/l.
Results of the second experiment:
Nominalconc.
(mg/l)
2 hours
24 hours
48 hours
72 hours
96 hours
Control 2
0 / 0
0 / 0
0 / 0
0 / 0
0 / 0
95
0 / 0
0 / 0
0 / 1
0 / 2
0 / 2
171
0 / 0
0 / 0
0 / 1
0 / 3
0 / 5
309
0 / 0
1 /9
1 / 5
2 / 10
7 / 10
555
0 / 0
9 / 10
10 / 10
- / -
- / -
1000
0 / 0
10 / 10
- / -
- / -
- / -
LC50
--
414
368
340
290
95 % C.I.
-
349 - 491
302 -448
141 -822
128 -657
- Validity criteria fulfilled:
- yes
- Remarks:
- No mortalities occured in the control during the test. The dissolved oxygen concentrations in the test media were always 8.32 mg/l or higher. The test substance concentrations in the analysed test media were in the range of 84.9 - 114.3 % of the nominal
- Conclusions:
- In this study Dimethylaminopropyl methacrylamide was found to be toxic to rainbow trout after 96 h at concentrations of 95 mg/L (LOEC 96 h) and higher. The LC50 (96 h) was calculated to be 290 mg/L. The NOEC after 96 hours was 50 mg/L.
- Executive summary:
In a 96-h acute toxicity study, rainbow trout (oncorhynchus mykiss) were exposed to Dimethylaminopropyl methacrylamide at nominal concentrations of 0 (control), 5, 9, 15, 28, 50, 95, 171, 309, 555, 1000 mg a.i./L under static/flow through conditions. The 96-h LC50 was 290 mg a.i./L. The NOEC value, based on mortality effects, was 50 mg a.i./L.
Based on the results of this study, Dimethylaminopropyl methacrylamide would be classified as not toxic to fish in accordance with GHS.
This toxicity study is classified as acceptable and satisfies the guideline requirements for acute toxicity to fish study.
Results:
Test organism size: 6.08 +/- 0.82 cm
Type: Static
LC50: 290 mg/L 95% C.I.: 128 - 657 mg /L
NOEC: 50 mg /L
Endpoint: mortality
Reference
Description of key information
The 96 h LC50 of in freshwater fish (Rainbow trout) was 290 mg/L (95% C.I.: 128 - 657 mg/L) mg/L according to OECD 203, static
conditions (RCC Umweltchemie GmbH & Co. KG, 1991) .
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 290 mg/L
Additional information
One acute freshwater study in fish is available for Dimethylaminopropyl methacrylamide which is consided reliable without restrictions (Klimisch score 1).
In a 96-h acute toxicity study, rainbow trout were exposed to Dimethylaminopropyl methacrylamide under static conditions according to OECD 203.
The 96-h LC50was 290 mg/L (95% CL (confidence limit): 128 - 657 mg/L, based on mortality). The 96 -h NOEC was 50 mg/L (based on mortality) (RCC Umweltchemie GmbH & Co. KG, 1991).Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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