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EC number: 801-260-5 | CAS number: 96383-55-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- yes
- Remarks:
- see Any other information on materials and methods incl. tables
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Health Care Inspectorate, Ministry of Health, Welfare and Sport, The Netherlands
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test substance was not completely soluble in test medium at the loading rates initially prepared. Therefore, test substance mixtures in Milli-RO water (tap water purified by reverse osmosis; Millipore Corp., Bedford, Mass., USA) with initial loading rates of 2.5 times the final loading rate were stirred in closed dark brown bottles for approximately 25 hours, except for the abiotic control which was stirred for 28.5 hours. Subsequently, to 200 mL of the prepared solutions, 16 mL synthetic medium, 250 mL sludge and Milli-RO water up to 500 mL were added resulting in the required loading rates. Optimal contact between the test substance and test organisms was ensured applying continuous aeration and stirring. - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Preparation of inoculum for exposure: The sludge was coarsely sieved (1 mm), washed and diluted with ISO-medium. A small amount of the sludge was
weighed and dried overnight at ca. 105°C to determine the amount of suspended solids (3.0 g/l of sludge, as used for the test). The pH was 8.0 on the day of testing. The batch of sludge was used one day after collection; therefore 50 ml of synthetic medium was added per litre of activated sludge at the end of the collection day. The sludge was kept aerated at test temperature until use.
- Concentration of suspended solids in the test mixture: 1.5 g/L in the final test mixture. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 3 h
- Remarks on exposure duration:
- Combined limit/range-finding test
- Test temperature:
- 18.2 - 20.1°C
- pH:
- before addition of sludge: 7.3 - 7.4
after the 3 hour exposure period: 7.2 - 8.5. - Dissolved oxygen:
- at the start above 60-70%
- Nominal and measured concentrations:
- Loading rates: 10, 100 and 1000 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: all glass bottles/vessels
- Aeration: clean, oil-free air, constantly
- No. of vessels per concentration (replicates): The highest loading rate was tested in triplicate, lower loading rates consisted of one replicate.
- No. of vessels per control (replicates): 6
- Biomass loading rate: 1.5 g/L in the final test mixture
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Tap-water purified by reverse osmosis
- Intervals of water quality measurement: Temperature and pH were measured at the start and at the end of the study.
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
After the 3-hour contact time, the oxygen consumption was recorded for a period of 10-15 minutes.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 10
- Justification for using less concentrations than requested by guideline: The test was performed as a limit test at 1000 mg/L. Additional lower concentrations were tested to be able to use the study as a range finding study, in case effects would have occurred. - Reference substance (positive control):
- yes
- Remarks:
- 1, 3,5-dichlorophenol
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: carbon and ammonium oxidation
- Key result
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: carbon and ammonium oxidation
- Details on results:
- - The combined limit/range-finding test showed no inhibition, 18% and an average of 5% inhibition of the respiration rate at a loading rate of 10, 100 and 1000 mg/l, respectively. The inhibition observed at a loading rate of 1000 mg/l was not statistically significant.
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: not reported
- Effect concentrations exceeding solubility of substance in test medium: not reported - Results with reference substance (positive control):
- - Results with reference substance valid? yes
- Relevant effect levels: EC50 (3h) = 14 mg/L - Reported statistics and error estimates:
- Two sample t-test: alpha= 0.05
- Executive summary:
One study is available on the toxicity of 3,3,4,4,5,5,6,6,7,7,8,8,8-tridecafluorooctyl 2-chloropropenoic acid ester (CAS 96383-55-0) to microorganisms. The study was conducted according to OECD guideline 209 and GLP (Desmares-Koopmans, 2013). The test was designed as a combined limit- and range finding study. The limit test loading 1000 mg/L was tested in triplicate. Additionally, single replicates of 10 and 100 mg/L loadings were tested, to be used for range finding, incase effects would occur. Activated sludge form a municipal sewage treatment plant, receiving predominantly domestic sewage, was exposed to the test substance for three hours, and the inhibition of respiration was analysed. The test showed no inhibition, 18% inhibition and an average of 5% inhibition of the respiration rate at a loading rates of 10, 100 and 1000 mg/L, respectively. The inhibition observed at a loading rate of 1000 mg/L was not statistically significant. Hence, the EC50 and NOEC can considered to be > 1000 mg/L and 1000 mg/L, respectively.
Reference
Table: Study results
Loading rate (mg/L) |
Respiration rate (mg O2/L h) |
Respiration rate (mg O2/g h) (1) |
% Inhibition of respiration relative to the control (mean) |
0 (Blank control, mean) |
39 |
26 |
- |
0 (Nitrification control) |
32 |
21 |
19 |
1000 (Abiotic control) |
0 |
0 |
0 |
1000 (Test substance with N-allylthiourea) |
32 |
21 |
1 (2) |
10 |
41 |
27 |
-4 |
100 |
32 |
21 |
18 |
1000 (mean) |
38 |
25 |
5 |
(1) The amount of suspended solids in the final test mixture was 1.5 g/L
(2) Relative to Nitrification control
Description of key information
EC50 (3h) > 1000 mg/L (OECD 209; activated sludge)
NOEC 1000 mg/L (OECD 209; activated sludge)
Key value for chemical safety assessment
- EC10 or NOEC for microorganisms:
- 1 000 mg/L
Additional information
One study is available on the toxicity of 3,3,4,4,5,5,6,6,7,7,8,8,8-tridecafluorooctyl 2-chloropropenoic acid ester (CAS 96383-55-0) to microorganisms. The study was conducted according to OECD guideline 209 and GLP (Desmares-Koopmans, 2013). The test was designed as a combined limit- and range finding study. The limit test loading 1000 mg/L was tested in triplicate. Additionally, single replicates of 10 and 100 mg/L loadings were tested, to be used for range finding, incase effects would occur. Activated sludge form a municipal sewage treatment plant, receiving predominantly domestic sewage, was exposed to the test substance for three hours, and the inhibition of respiration was analysed. The test showed no inhibition, 18% inhibition and an average of 5% inhibition of the respiration rate at a loading rates of 10, 100 and 1000 mg/L, respectively. The inhibition observed at a loading rate of 1000 mg/L was not statistically significant. Hence, the EC50 and NOEC can considered to be > 1000 mg/L and 1000 mg/L, respectively.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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