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EC number: 266-257-8 | CAS number: 66215-27-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Long-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1983/06/08 to 1983/06/29
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
- Justification for type of information:
- Study was not performed under GLP conditions. The guidelines followed in this study are not stated. There are no deviations in the study.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 984
- Report date:
- 1984
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 211 (Daphnia magna Reproduction Test)
- GLP compliance:
- no
Test material
- Reference substance name:
- N-cyclopropyl-1,3,5-triazine-2,4,6-triamine
- EC Number:
- 266-257-8
- EC Name:
- N-cyclopropyl-1,3,5-triazine-2,4,6-triamine
- Cas Number:
- 66215-27-8
- Molecular formula:
- C6H10N6
- IUPAC Name:
- N2-cyclopropyl-1,3,5-triazine-2,4,6-triamine
- Test material form:
- solid: particulate/powder
- Remarks:
- Colorless powder
Constituent 1
- Specific details on test material used for the study:
- The test material is cyromazine.
Sampling and analysis
- Analytical monitoring:
- yes
- Remarks:
- Analytical determinations of test substance concentrations were made on sample collected on days 1, 7, 14 and 21.
- Details on sampling:
- Ten test organisms were assigned to each test beaker, totaling 40 test organisms per concentration and control. Live animals were recorded and dead animals removed daily, except on Days 3, and 6. The number of live animals was recorded daily (except on days 3, 4 and 6) and the dead animals removed. From day 10 onwards, live animals were counted and newly hatched neonates counted and removed. At the end of the 21-day exposure period the surviving adults in each test chamber were removed, preserved in alcohol and their length was determined.
Test solutions
- Vehicle:
- no
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- The Daphnia Magna used for this test are static cultures maintained on trout chow at 20-24 °C. About 7 days prior to testing, large adults were gently netted from stock cultures and placed in the test chambers (~20/chamber) under flow-through conditions. The daphnia were fed a mixture of the unicellular algae Chlamydomonas reinhardtii and a solution of 'Cerophyl', the food was supplied automatically as the test solution was refreshed.
Study design
- Test type:
- flow-through
- Water media type:
- other: dechlorinated, biologically aged tap water
- Total exposure duration:
- 21 d
Test conditions
- Test temperature:
- 20 ± 2°C
- Nominal and measured concentrations:
- Nominal concentrations (mg/L): 0.23, 0.46, 0.91, 1.82 and 3.64
Mean measured concentrations (mg/L): 0.31, 0.64, 0.96, 1.8, 3.7 - Details on test conditions:
- 4 test vessels were used, each vessel containing 10 neonates at age <48 hours. The test chamber were 1500ml glass beaker with 330-micron nitex net collars. 200ml of fresh test solution (or control) was pumped through test system every 15 minutes during exposure period
The test chambers were cleaned twice weekly for the duration of the experiment when the chambers were being cleaned the Daphnia magna were gently pipetted into 100mL of test solution in a separate vessel. For the 21 days duration of the test, the test vessels were subjected to a 16 hour photoperiod and maintained in a water bath set at 20 ± 2°C.
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- > 0.31 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- act. ingr.
- Basis for effect:
- growth
- Remarks:
- adult daphnia body lengths
- Key result
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- > 0.64 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- act. ingr.
- Basis for effect:
- reproduction: production of clutches per surviving parent organism {for L. stagnalis, TG 243}
- Details on results:
- There was no statistically significant difference in mortality (Scheffées multiple comparison) between the control treatments and the 0.31 and 0 64 mg/L test material treatments However, test material concentrations of 0.96 mg/L and above resulted in a statistically significant increase in mortality on day 21.
- Reported statistics and error estimates:
- There was no statistical difference in length of the surviving Daphnia magna in the 0.31 mg/L test material treatment. However, based on a one-tailed Dunnett's test, treatment with 0.64 mg/L test material resulted in a statistically significant reduction in length compared to the control treatment (3.94 mm compared to 4.43 mm). No young were produced in the 0.96, 1.8 and 3.7 mg/L test material treatments but in the 0.31 and 0.64 mg/L treatments young were released from day 10 onwards. An initial one-way analysis of variance detected no significant differences in reproduction however, a subsequent analysis using the one-tailed Dunnett's test showed a significant difference between the control and the 0 64 mg/L treatment.
Any other information on results incl. tables
- Significant difference from the control (Scheffés multiple comparison test, P= 0.05)
- Significantly different from the control, one tailed Dunnett's test (P= 0.05)
Analytical findings
The measured concentration of test material in some of the test chambers was outside the 80-120% limit and therefore the measured concentration will be used The mean measured concentrations (mg/L) are: 0.31, 0.64, 0.96, 1.8, 3.7 compared to the nominal concentrations (mg/L) of 0.23, 0.46, 0.91, 1.82 and 3.64.
Table 1
Measured cyromazine levels during the Daphnia magna chronic toxicity study
Nominal test substance con (mg/L) | Measured concentrations | |||||||||
Day 1 | Day 7 | Day 14 | Day 21 | Mean | ||||||
mg/L | % of nominal | mg/L | % of nominal | mg/L | % of nominal | mg/L | % of nominal | mg/L | % of nominal | |
Control | <0.2 7 | - | <0.27 | - | <0.27 | - | <0.27 | - | <0.27 | - |
0 23 | 0.35 | 152.2 | 0.28 | 121.7 | <0.27 | - | 0.31 | 134.8 | 0.31 | 134.8 |
0 46 | 0.75 | 163.0 | 0.56 | 121.7 | 0.63 | 137.0 | 0.63 | 137.0 | 0.64 | 139.1 |
0 91 | 0.88 | 96.7 | 0.81 | 89.0 | 1.2 | 131.9 | nd | - | 0.96 | 105.5 |
1 82 | 2.1 | 115.4 | 1.4 | 76.9 | nd | - | nd | - | 1.8 | 98.9 |
3 64 | 4.3 | 118.1 | 3.1 | 85.2 | nd | - | nd | - | 3.7 | 101.6 |
nd: not determined as all test organisms in those concentrations were dead Detection limit was 0.27 mg/L
Table 2
Survival of Daphnia magna, exposed under flow-through conditions, to cyromazine in a 21-day chronic study
Mean measured treatment concentrations Test material (mg/L) | Mean number of living Daphnia magna | Mean % living Daphnia magna (day 21) | ||
Day 7 | Day 14 | Day 21 | ||
Control | 9.3 | 8.3 | 8 | 80.0 |
0.31 | 9.5 | 9.3 | 8.5 | 85.0 |
0.64 | 9 | 8 | 6.8 | 67.5 |
0.96 | 3.5 | 0.5 | 0 | 0 0* |
1 8 | 0 5 | 0 | 0 | 0 0* |
3 7 | 0 | 0 | 0 | 0 0* |
Table 3
Reproductive performance of Daphnia magna exposed under flow-through conditions, to test material in a 21-day chronic study
Mean measured conc. Test material (mg/L) | Mean no. Young/test chamber | No. 8reeding Adults | No. of Adult Reproduction Days | Young/Adult reproductive day (Mean ) |
Control | 423 75 | 6-10 | 72-120 | 4.33 |
0.31 | 414 75 | 7-10 | 101-120 | 3.84 |
0.64 | 262 | 6-10 | 81-117 | 2.91* |
0.96 | 0 | - | - | - |
1.8 | 0 | - | - | - |
3.7 | 0 | - | - | - |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- not specified
- Conclusions:
- In a 21-day chronic flow through study the NOEC for reproduction of Daphnia magna is > 0.64 mg/L cyromazine and for length of surviving adults the NOEC is > 0.31 mg/L cyromazine. In summary the NOEC using length and reproduction is 0.45 mg/L.
- Executive summary:
The effect of cyromazine on the mortality, growth and reproductive success of Daphnia magna was investigated in a 21-day chronic, flow-through study The test substance was tested at 0.23, 0.46, 0.91, 1.82 and 3.64 mg/L. All treatments were made up in dechlorinated, biologically aged tap water. Since there were no guideline used in this study, there was no validity criteria that was met.
In a 21-day chronic flow through study the NOEC for reproduction of Daphnia magna is > 0.64 mg/L cyromazine and for length of surviving adults the NOEC is > 0.31 mg/L cyromazine.
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